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Optimal Media for In-Vitro Regeneration of Two Local Genotypes of Chili Pepper (Capsicum annuum L.) from West Sumatera

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@article{IJASEIT1003,
   author = {- Renfiyeni and Lily Syukriani and Alfi Asben and - Jamsari},
   title = {Optimal Media for In-Vitro Regeneration of Two Local Genotypes of Chili Pepper (Capsicum annuum L.)  from West Sumatera},
   journal = {International Journal on Advanced Science, Engineering and Information Technology},
   volume = {7},
   number = {3},
   year = {2017},
   pages = {904--909},
   keywords = {calli induction; shoot and root induction; Capsicum annuum; genetic transformation; local genotype},
   abstract = {Genetic engineering strategy in chilli pepper (Capsicum annuum L.) has been applied so far in order to improve its genetic capacity for instance against some important diseases. This strategy needs an established system for calli preparation as well as transformant regeneration. In most cases, this necessity is genotype dependent especially for some local genotypes. Based on this, we optimized media for calli induction, shoot and root regeneration of our three local cultivars namely Kampung, Kopay and Trisula. Media combinations basically were composed of MS  and combined with some growth factors for instance IAA, BAP, NAA and TDZ with different combinations for each calli induction, shoot and root regeneration. All media compositions were supplemented with 30 GL-1 sucrose. The best medium for calli induction was MS media supplemented with 4 mgL-1 BAP, 0.5 mgL-1 IAA and L2 vitamin (Thiamin-HCl and Pyrodoxine-HCl). While the best medium for shoot induction and elongation was MS supplemented with 1mgL-1 BAP, 5 mgL-1 AgNO3, 2 mgL-1 GA3, 2 mgL-1 Calcium Pantotenate and L2 vitamin.  Furthermore, the best medium for root induction and elongation was WPM (Woody Plant Medium) supplemented with 0.4 mgL-1 NAA. This finding should provide optimal media for calli induction and shoot-root regeneration for the three local cultivars Kampung, Kopay and Trisula.},
   issn = {2088-5334},
   publisher = {INSIGHT - Indonesian Society for Knowledge and Human Development},
   url = {http://ijaseit.insightsociety.org/index.php?option=com_content&view=article&id=9&Itemid=1&article_id=1003},
   doi = {10.18517/ijaseit.7.3.1003}
}

EndNote

%A Renfiyeni, -
%A Syukriani, Lily
%A Asben, Alfi
%A Jamsari, -
%D 2017
%T Optimal Media for In-Vitro Regeneration of Two Local Genotypes of Chili Pepper (Capsicum annuum L.)  from West Sumatera
%B 2017
%9 calli induction; shoot and root induction; Capsicum annuum; genetic transformation; local genotype
%! Optimal Media for In-Vitro Regeneration of Two Local Genotypes of Chili Pepper (Capsicum annuum L.)  from West Sumatera
%K calli induction; shoot and root induction; Capsicum annuum; genetic transformation; local genotype
%X Genetic engineering strategy in chilli pepper (Capsicum annuum L.) has been applied so far in order to improve its genetic capacity for instance against some important diseases. This strategy needs an established system for calli preparation as well as transformant regeneration. In most cases, this necessity is genotype dependent especially for some local genotypes. Based on this, we optimized media for calli induction, shoot and root regeneration of our three local cultivars namely Kampung, Kopay and Trisula. Media combinations basically were composed of MS  and combined with some growth factors for instance IAA, BAP, NAA and TDZ with different combinations for each calli induction, shoot and root regeneration. All media compositions were supplemented with 30 GL-1 sucrose. The best medium for calli induction was MS media supplemented with 4 mgL-1 BAP, 0.5 mgL-1 IAA and L2 vitamin (Thiamin-HCl and Pyrodoxine-HCl). While the best medium for shoot induction and elongation was MS supplemented with 1mgL-1 BAP, 5 mgL-1 AgNO3, 2 mgL-1 GA3, 2 mgL-1 Calcium Pantotenate and L2 vitamin.  Furthermore, the best medium for root induction and elongation was WPM (Woody Plant Medium) supplemented with 0.4 mgL-1 NAA. This finding should provide optimal media for calli induction and shoot-root regeneration for the three local cultivars Kampung, Kopay and Trisula.
%U http://ijaseit.insightsociety.org/index.php?option=com_content&view=article&id=9&Itemid=1&article_id=1003
%R doi:10.18517/ijaseit.7.3.1003
%J International Journal on Advanced Science, Engineering and Information Technology
%V 7
%N 3
%@ 2088-5334

IEEE

- Renfiyeni,Lily Syukriani,Alfi Asben and - Jamsari,"Optimal Media for In-Vitro Regeneration of Two Local Genotypes of Chili Pepper (Capsicum annuum L.)  from West Sumatera," International Journal on Advanced Science, Engineering and Information Technology, vol. 7, no. 3, pp. 904-909, 2017. [Online]. Available: http://dx.doi.org/10.18517/ijaseit.7.3.1003.

RefMan/ProCite (RIS)

TY  - JOUR
AU  - Renfiyeni, -
AU  - Syukriani, Lily
AU  - Asben, Alfi
AU  - Jamsari, -
PY  - 2017
TI  - Optimal Media for In-Vitro Regeneration of Two Local Genotypes of Chili Pepper (Capsicum annuum L.)  from West Sumatera
JF  - International Journal on Advanced Science, Engineering and Information Technology; Vol. 7 (2017) No. 3
Y2  - 2017
SP  - 904
EP  - 909
SN  - 2088-5334
PB  - INSIGHT - Indonesian Society for Knowledge and Human Development
KW  - calli induction; shoot and root induction; Capsicum annuum; genetic transformation; local genotype
N2  - Genetic engineering strategy in chilli pepper (Capsicum annuum L.) has been applied so far in order to improve its genetic capacity for instance against some important diseases. This strategy needs an established system for calli preparation as well as transformant regeneration. In most cases, this necessity is genotype dependent especially for some local genotypes. Based on this, we optimized media for calli induction, shoot and root regeneration of our three local cultivars namely Kampung, Kopay and Trisula. Media combinations basically were composed of MS  and combined with some growth factors for instance IAA, BAP, NAA and TDZ with different combinations for each calli induction, shoot and root regeneration. All media compositions were supplemented with 30 GL-1 sucrose. The best medium for calli induction was MS media supplemented with 4 mgL-1 BAP, 0.5 mgL-1 IAA and L2 vitamin (Thiamin-HCl and Pyrodoxine-HCl). While the best medium for shoot induction and elongation was MS supplemented with 1mgL-1 BAP, 5 mgL-1 AgNO3, 2 mgL-1 GA3, 2 mgL-1 Calcium Pantotenate and L2 vitamin.  Furthermore, the best medium for root induction and elongation was WPM (Woody Plant Medium) supplemented with 0.4 mgL-1 NAA. This finding should provide optimal media for calli induction and shoot-root regeneration for the three local cultivars Kampung, Kopay and Trisula.
UR  - http://ijaseit.insightsociety.org/index.php?option=com_content&view=article&id=9&Itemid=1&article_id=1003
DO  - 10.18517/ijaseit.7.3.1003

RefWorks

RT Journal Article
ID 1003
A1 Renfiyeni, -
A1 Syukriani, Lily
A1 Asben, Alfi
A1 Jamsari, -
T1 Optimal Media for In-Vitro Regeneration of Two Local Genotypes of Chili Pepper (Capsicum annuum L.)  from West Sumatera
JF International Journal on Advanced Science, Engineering and Information Technology
VO 7
IS 3
YR 2017
SP 904
OP 909
SN 2088-5334
PB INSIGHT - Indonesian Society for Knowledge and Human Development
K1 calli induction; shoot and root induction; Capsicum annuum; genetic transformation; local genotype
AB Genetic engineering strategy in chilli pepper (Capsicum annuum L.) has been applied so far in order to improve its genetic capacity for instance against some important diseases. This strategy needs an established system for calli preparation as well as transformant regeneration. In most cases, this necessity is genotype dependent especially for some local genotypes. Based on this, we optimized media for calli induction, shoot and root regeneration of our three local cultivars namely Kampung, Kopay and Trisula. Media combinations basically were composed of MS  and combined with some growth factors for instance IAA, BAP, NAA and TDZ with different combinations for each calli induction, shoot and root regeneration. All media compositions were supplemented with 30 GL-1 sucrose. The best medium for calli induction was MS media supplemented with 4 mgL-1 BAP, 0.5 mgL-1 IAA and L2 vitamin (Thiamin-HCl and Pyrodoxine-HCl). While the best medium for shoot induction and elongation was MS supplemented with 1mgL-1 BAP, 5 mgL-1 AgNO3, 2 mgL-1 GA3, 2 mgL-1 Calcium Pantotenate and L2 vitamin.  Furthermore, the best medium for root induction and elongation was WPM (Woody Plant Medium) supplemented with 0.4 mgL-1 NAA. This finding should provide optimal media for calli induction and shoot-root regeneration for the three local cultivars Kampung, Kopay and Trisula.
LK http://ijaseit.insightsociety.org/index.php?option=com_content&view=article&id=9&Itemid=1&article_id=1003
DO  - 10.18517/ijaseit.7.3.1003