Cite Article

The Effect of Auxin and Cytokinin on Black Orchid Hybrid (Coelogyne pandurata Lindley) in Vitro

Choose citation format

BibTeX

@article{IJASEIT14192,
   author = {Sri Hartati and Retna B.A and Brigita R.H and Ongko Cahyono},
   title = {The Effect of Auxin and Cytokinin on Black Orchid Hybrid (Coelogyne pandurata Lindley) in Vitro},
   journal = {International Journal on Advanced Science, Engineering and Information Technology},
   volume = {12},
   number = {3},
   year = {2022},
   pages = {981--986},
   keywords = {Effect; NAA; BA; Coelogyne pandurate; Coelogyne rumphii.},
   abstract = {The Orchid is an ornamental plant with high economic value due to the emergence of unique varieties. These varieties are produced from conventional breeding methods of crossing and selection. Meanwhile, the propagation of orchids through in vivo hatcheries is constrained because the seeds are too small to contain the endosperm. Tissue culture is an alternative for the propagation of orchids Efforts to propagate orchids through tissue culture is carried out by adding Growth Regulatory Substances such as auxins groups, namely NAA, IBA, IAA, and cytokinin groups, namely BA, BAP, kinetin, and TDZ to MS media, to enable the plants to take up adequate nutrients for growth. Therefore, this study aims to determine the effect of various concentrations of NAA and BA on the best ½ MS media for the growth of orchid subcultures. A completely randomized design pattern (CRD) of 2 factors with 12 treatment combinations and four replications were used, totaling 48 experimental units. The results showed that the addition of 1ppm NAA had the highest number of roots and length, while the addition of BA at 3ppm had the highest height, and the combination of 1ppm NAA and BA 3ppm treatment produced the highest number of leaves (42 sheets) and shoots. Based on the results, the addition of NAA above 3ppm followed by BA at 6ppm can produce more leaves and shoots, while NAA above 3ppm can produce higher plant height.},
   issn = {2088-5334},
   publisher = {INSIGHT - Indonesian Society for Knowledge and Human Development},
   url = {http://ijaseit.insightsociety.org/index.php?option=com_content&view=article&id=9&Itemid=1&article_id=14192},
   doi = {10.18517/ijaseit.12.3.14192}
}

EndNote

%A Hartati, Sri
%A B.A, Retna
%A R.H, Brigita
%A Cahyono, Ongko
%D 2022
%T The Effect of Auxin and Cytokinin on Black Orchid Hybrid (Coelogyne pandurata Lindley) in Vitro
%B 2022
%9 Effect; NAA; BA; Coelogyne pandurate; Coelogyne rumphii.
%! The Effect of Auxin and Cytokinin on Black Orchid Hybrid (Coelogyne pandurata Lindley) in Vitro
%K Effect; NAA; BA; Coelogyne pandurate; Coelogyne rumphii.
%X The Orchid is an ornamental plant with high economic value due to the emergence of unique varieties. These varieties are produced from conventional breeding methods of crossing and selection. Meanwhile, the propagation of orchids through in vivo hatcheries is constrained because the seeds are too small to contain the endosperm. Tissue culture is an alternative for the propagation of orchids Efforts to propagate orchids through tissue culture is carried out by adding Growth Regulatory Substances such as auxins groups, namely NAA, IBA, IAA, and cytokinin groups, namely BA, BAP, kinetin, and TDZ to MS media, to enable the plants to take up adequate nutrients for growth. Therefore, this study aims to determine the effect of various concentrations of NAA and BA on the best ½ MS media for the growth of orchid subcultures. A completely randomized design pattern (CRD) of 2 factors with 12 treatment combinations and four replications were used, totaling 48 experimental units. The results showed that the addition of 1ppm NAA had the highest number of roots and length, while the addition of BA at 3ppm had the highest height, and the combination of 1ppm NAA and BA 3ppm treatment produced the highest number of leaves (42 sheets) and shoots. Based on the results, the addition of NAA above 3ppm followed by BA at 6ppm can produce more leaves and shoots, while NAA above 3ppm can produce higher plant height.
%U http://ijaseit.insightsociety.org/index.php?option=com_content&view=article&id=9&Itemid=1&article_id=14192
%R doi:10.18517/ijaseit.12.3.14192
%J International Journal on Advanced Science, Engineering and Information Technology
%V 12
%N 3
%@ 2088-5334

IEEE

Sri Hartati,Retna B.A,Brigita R.H and Ongko Cahyono,"The Effect of Auxin and Cytokinin on Black Orchid Hybrid (Coelogyne pandurata Lindley) in Vitro," International Journal on Advanced Science, Engineering and Information Technology, vol. 12, no. 3, pp. 981-986, 2022. [Online]. Available: http://dx.doi.org/10.18517/ijaseit.12.3.14192.

RefMan/ProCite (RIS)

TY  - JOUR
AU  - Hartati, Sri
AU  - B.A, Retna
AU  - R.H, Brigita
AU  - Cahyono, Ongko
PY  - 2022
TI  - The Effect of Auxin and Cytokinin on Black Orchid Hybrid (Coelogyne pandurata Lindley) in Vitro
JF  - International Journal on Advanced Science, Engineering and Information Technology; Vol. 12 (2022) No. 3
Y2  - 2022
SP  - 981
EP  - 986
SN  - 2088-5334
PB  - INSIGHT - Indonesian Society for Knowledge and Human Development
KW  - Effect; NAA; BA; Coelogyne pandurate; Coelogyne rumphii.
N2  - The Orchid is an ornamental plant with high economic value due to the emergence of unique varieties. These varieties are produced from conventional breeding methods of crossing and selection. Meanwhile, the propagation of orchids through in vivo hatcheries is constrained because the seeds are too small to contain the endosperm. Tissue culture is an alternative for the propagation of orchids Efforts to propagate orchids through tissue culture is carried out by adding Growth Regulatory Substances such as auxins groups, namely NAA, IBA, IAA, and cytokinin groups, namely BA, BAP, kinetin, and TDZ to MS media, to enable the plants to take up adequate nutrients for growth. Therefore, this study aims to determine the effect of various concentrations of NAA and BA on the best ½ MS media for the growth of orchid subcultures. A completely randomized design pattern (CRD) of 2 factors with 12 treatment combinations and four replications were used, totaling 48 experimental units. The results showed that the addition of 1ppm NAA had the highest number of roots and length, while the addition of BA at 3ppm had the highest height, and the combination of 1ppm NAA and BA 3ppm treatment produced the highest number of leaves (42 sheets) and shoots. Based on the results, the addition of NAA above 3ppm followed by BA at 6ppm can produce more leaves and shoots, while NAA above 3ppm can produce higher plant height.
UR  - http://ijaseit.insightsociety.org/index.php?option=com_content&view=article&id=9&Itemid=1&article_id=14192
DO  - 10.18517/ijaseit.12.3.14192

RefWorks

RT Journal Article
ID 14192
A1 Hartati, Sri
A1 B.A, Retna
A1 R.H, Brigita
A1 Cahyono, Ongko
T1 The Effect of Auxin and Cytokinin on Black Orchid Hybrid (Coelogyne pandurata Lindley) in Vitro
JF International Journal on Advanced Science, Engineering and Information Technology
VO 12
IS 3
YR 2022
SP 981
OP 986
SN 2088-5334
PB INSIGHT - Indonesian Society for Knowledge and Human Development
K1 Effect; NAA; BA; Coelogyne pandurate; Coelogyne rumphii.
AB The Orchid is an ornamental plant with high economic value due to the emergence of unique varieties. These varieties are produced from conventional breeding methods of crossing and selection. Meanwhile, the propagation of orchids through in vivo hatcheries is constrained because the seeds are too small to contain the endosperm. Tissue culture is an alternative for the propagation of orchids Efforts to propagate orchids through tissue culture is carried out by adding Growth Regulatory Substances such as auxins groups, namely NAA, IBA, IAA, and cytokinin groups, namely BA, BAP, kinetin, and TDZ to MS media, to enable the plants to take up adequate nutrients for growth. Therefore, this study aims to determine the effect of various concentrations of NAA and BA on the best ½ MS media for the growth of orchid subcultures. A completely randomized design pattern (CRD) of 2 factors with 12 treatment combinations and four replications were used, totaling 48 experimental units. The results showed that the addition of 1ppm NAA had the highest number of roots and length, while the addition of BA at 3ppm had the highest height, and the combination of 1ppm NAA and BA 3ppm treatment produced the highest number of leaves (42 sheets) and shoots. Based on the results, the addition of NAA above 3ppm followed by BA at 6ppm can produce more leaves and shoots, while NAA above 3ppm can produce higher plant height.
LK http://ijaseit.insightsociety.org/index.php?option=com_content&view=article&id=9&Itemid=1&article_id=14192
DO  - 10.18517/ijaseit.12.3.14192