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Biodiesel Synthesis through the Non-Alcohol Route using the Immobilized Lipase Enzyme from Bacillus subtilis as Biocatalyst

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@article{IJASEIT14784,
   author = {Afrah Alatas and Dwini Normayulisa Putri and Meka Saima Perdani and Tania Surya Utami and Heri Hermansyah},
   title = {Biodiesel Synthesis through the Non-Alcohol Route using the Immobilized Lipase Enzyme from Bacillus subtilis as Biocatalyst},
   journal = {International Journal on Advanced Science, Engineering and Information Technology},
   volume = {11},
   number = {3},
   year = {2021},
   pages = {1239--1245},
   keywords = {Bacillus subtilis; biodiesel; immobilization; interesterification; lipase.},
   abstract = {

Biodiesel is one alternative energy that might be promising to substitute the petroleum-based fuel due to its biodegradability and less toxic level. Biodiesel can be synthesized through the non-alcohol route by substituting the alcohol with methyl acetate using lipase enzyme as the Biocatalyst, which may give less by-product during the reaction. In this research, lipase from Bacillus subtilis has been produced and prepared in a crude, dry and immobilized form.  It is used as the Biocatalyst for biodiesel synthesis. Several variables were investigated for obtaining the optimum condition in producing biodiesel, including the form of lipase enzyme, the variation of molar ratio between oil and methyl acetate, and the enzyme concentration. The immobilized Bacillus subtilis lipase enzyme loading was also calculated to know how many percent of the lipase is successfully immobilized. The biodiesel produced using lipase from Bacillus subtilis will then be compared with the biodiesel produced using Candida rugosa commercial lipase. Based on the experimental, the immobilized lipase with 1:12 of oil to methyl acetate and enzyme concentration of 2% gives the highest biodiesel yield among the others, as much as 53.99%. This yield was higher than the biodiesel produced using Candida rugosa lipase. In conclusion, the immobilized lipase from Bacillus subtilis is one of the promising Biocatalyst for biodiesel production.

},    issn = {2088-5334},    publisher = {INSIGHT - Indonesian Society for Knowledge and Human Development},    url = {http://ijaseit.insightsociety.org/index.php?option=com_content&view=article&id=9&Itemid=1&article_id=14784},    doi = {10.18517/ijaseit.11.3.14784} }

EndNote

%A Alatas, Afrah
%A Putri, Dwini Normayulisa
%A Perdani, Meka Saima
%A Utami, Tania Surya
%A Hermansyah, Heri
%D 2021
%T Biodiesel Synthesis through the Non-Alcohol Route using the Immobilized Lipase Enzyme from Bacillus subtilis as Biocatalyst
%B 2021
%9 Bacillus subtilis; biodiesel; immobilization; interesterification; lipase.
%! Biodiesel Synthesis through the Non-Alcohol Route using the Immobilized Lipase Enzyme from Bacillus subtilis as Biocatalyst
%K Bacillus subtilis; biodiesel; immobilization; interesterification; lipase.
%X 

Biodiesel is one alternative energy that might be promising to substitute the petroleum-based fuel due to its biodegradability and less toxic level. Biodiesel can be synthesized through the non-alcohol route by substituting the alcohol with methyl acetate using lipase enzyme as the Biocatalyst, which may give less by-product during the reaction. In this research, lipase from Bacillus subtilis has been produced and prepared in a crude, dry and immobilized form.  It is used as the Biocatalyst for biodiesel synthesis. Several variables were investigated for obtaining the optimum condition in producing biodiesel, including the form of lipase enzyme, the variation of molar ratio between oil and methyl acetate, and the enzyme concentration. The immobilized Bacillus subtilis lipase enzyme loading was also calculated to know how many percent of the lipase is successfully immobilized. The biodiesel produced using lipase from Bacillus subtilis will then be compared with the biodiesel produced using Candida rugosa commercial lipase. Based on the experimental, the immobilized lipase with 1:12 of oil to methyl acetate and enzyme concentration of 2% gives the highest biodiesel yield among the others, as much as 53.99%. This yield was higher than the biodiesel produced using Candida rugosa lipase. In conclusion, the immobilized lipase from Bacillus subtilis is one of the promising Biocatalyst for biodiesel production.

%U http://ijaseit.insightsociety.org/index.php?option=com_content&view=article&id=9&Itemid=1&article_id=14784 %R doi:10.18517/ijaseit.11.3.14784 %J International Journal on Advanced Science, Engineering and Information Technology %V 11 %N 3 %@ 2088-5334

IEEE

Afrah Alatas,Dwini Normayulisa Putri,Meka Saima Perdani,Tania Surya Utami and Heri Hermansyah,"Biodiesel Synthesis through the Non-Alcohol Route using the Immobilized Lipase Enzyme from Bacillus subtilis as Biocatalyst," International Journal on Advanced Science, Engineering and Information Technology, vol. 11, no. 3, pp. 1239-1245, 2021. [Online]. Available: http://dx.doi.org/10.18517/ijaseit.11.3.14784.

RefMan/ProCite (RIS)

TY  - JOUR
AU  - Alatas, Afrah
AU  - Putri, Dwini Normayulisa
AU  - Perdani, Meka Saima
AU  - Utami, Tania Surya
AU  - Hermansyah, Heri
PY  - 2021
TI  - Biodiesel Synthesis through the Non-Alcohol Route using the Immobilized Lipase Enzyme from Bacillus subtilis as Biocatalyst
JF  - International Journal on Advanced Science, Engineering and Information Technology; Vol. 11 (2021) No. 3
Y2  - 2021
SP  - 1239
EP  - 1245
SN  - 2088-5334
PB  - INSIGHT - Indonesian Society for Knowledge and Human Development
KW  - Bacillus subtilis; biodiesel; immobilization; interesterification; lipase.
N2  - 

Biodiesel is one alternative energy that might be promising to substitute the petroleum-based fuel due to its biodegradability and less toxic level. Biodiesel can be synthesized through the non-alcohol route by substituting the alcohol with methyl acetate using lipase enzyme as the Biocatalyst, which may give less by-product during the reaction. In this research, lipase from Bacillus subtilis has been produced and prepared in a crude, dry and immobilized form.  It is used as the Biocatalyst for biodiesel synthesis. Several variables were investigated for obtaining the optimum condition in producing biodiesel, including the form of lipase enzyme, the variation of molar ratio between oil and methyl acetate, and the enzyme concentration. The immobilized Bacillus subtilis lipase enzyme loading was also calculated to know how many percent of the lipase is successfully immobilized. The biodiesel produced using lipase from Bacillus subtilis will then be compared with the biodiesel produced using Candida rugosa commercial lipase. Based on the experimental, the immobilized lipase with 1:12 of oil to methyl acetate and enzyme concentration of 2% gives the highest biodiesel yield among the others, as much as 53.99%. This yield was higher than the biodiesel produced using Candida rugosa lipase. In conclusion, the immobilized lipase from Bacillus subtilis is one of the promising Biocatalyst for biodiesel production.

UR - http://ijaseit.insightsociety.org/index.php?option=com_content&view=article&id=9&Itemid=1&article_id=14784 DO - 10.18517/ijaseit.11.3.14784

RefWorks

RT Journal Article
ID 14784
A1 Alatas, Afrah
A1 Putri, Dwini Normayulisa
A1 Perdani, Meka Saima
A1 Utami, Tania Surya
A1 Hermansyah, Heri
T1 Biodiesel Synthesis through the Non-Alcohol Route using the Immobilized Lipase Enzyme from Bacillus subtilis as Biocatalyst
JF International Journal on Advanced Science, Engineering and Information Technology
VO 11
IS 3
YR 2021
SP 1239
OP 1245
SN 2088-5334
PB INSIGHT - Indonesian Society for Knowledge and Human Development
K1 Bacillus subtilis; biodiesel; immobilization; interesterification; lipase.
AB 

Biodiesel is one alternative energy that might be promising to substitute the petroleum-based fuel due to its biodegradability and less toxic level. Biodiesel can be synthesized through the non-alcohol route by substituting the alcohol with methyl acetate using lipase enzyme as the Biocatalyst, which may give less by-product during the reaction. In this research, lipase from Bacillus subtilis has been produced and prepared in a crude, dry and immobilized form.  It is used as the Biocatalyst for biodiesel synthesis. Several variables were investigated for obtaining the optimum condition in producing biodiesel, including the form of lipase enzyme, the variation of molar ratio between oil and methyl acetate, and the enzyme concentration. The immobilized Bacillus subtilis lipase enzyme loading was also calculated to know how many percent of the lipase is successfully immobilized. The biodiesel produced using lipase from Bacillus subtilis will then be compared with the biodiesel produced using Candida rugosa commercial lipase. Based on the experimental, the immobilized lipase with 1:12 of oil to methyl acetate and enzyme concentration of 2% gives the highest biodiesel yield among the others, as much as 53.99%. This yield was higher than the biodiesel produced using Candida rugosa lipase. In conclusion, the immobilized lipase from Bacillus subtilis is one of the promising Biocatalyst for biodiesel production.

LK http://ijaseit.insightsociety.org/index.php?option=com_content&view=article&id=9&Itemid=1&article_id=14784 DO - 10.18517/ijaseit.11.3.14784