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Isolation of Lactobacillus salivarius from Children and Purification of Bacteriocin to Inhibition Cancer Cell in Vitro

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@article{IJASEIT24,
   author = {Waleed K. M. Al-Tememy and Ibraem A. Al-Ani and Mohammed Q. Al-Ani and Nadia T. Al-Alusi and Salmah Ismail},
   title = {Isolation of Lactobacillus salivarius from Children and Purification of Bacteriocin to Inhibition Cancer Cell in Vitro},
   journal = {International Journal on Advanced Science, Engineering and Information Technology},
   volume = {1},
   number = {1},
   year = {2011},
   pages = {104--108},
   keywords = {Lactobacillus salivarius; Bacteriocins; ion-exchange; tumor cell line},
   abstract = {Bacteria being used to make anticancer agents could provide an extra source of lead compounds for the pharmaceutical industry.  Bacterium Lactobacillus salivarius produce compounds that selectively inhibit growth of human cancer cells Lactobacillus salivarius naturally produces a compound called Bacteriocins.  Bacteriocins are bacterial proteins produced to prevent the growth of competing microorganisms in a particular biological niche and we can use it as antineoplastic. The aim of this study was to isolate bacteriocin produced by lactic acid bacteria. A preparation of bacteriocin from a strain Lactobacillus salivarius has long been shown to have antineoplastic activity against a variety of human tumor and animal tumor cell lines in vitro. A total of 60 LAB  were isolated from children stool 45 isolate showed a clear antimicrobial activity against indicator strain Streptococcus aureus and by used sodium phosphate buffer (pH8) from an 80% ammonium sulfate precipitate. The inhibition  activity was determent by well diffusion assay method technique, Bacteriocin purification processes were carried out by using ion-exchange (Trisacryl SP) and gel filtration chromatography (Sephacryl – S300). The apparent molecular mass of partially purified bacteriocin was 15. 848 kDa,  Cell Culture was maintained in RPMI 1640 medium supplemented with 10% (vol/vol) fetal calf serum,  Cytotoxicity of bacteriocin was assessed on human cell line (RD) and animal cell line (MDCK) cell viability after incubation for 48 h in medium containing 500AU/ml (1.15 mg/ml). Both cell types used in this study were sensitive to bacteriocin and the bacteriocin appeared to inhibit proliferation of tumor cell line. The animal cell line was more sensitivity than human cell line.},
   issn = {2088-5334},
   publisher = {INSIGHT - Indonesian Society for Knowledge and Human Development},
   url = {http://ijaseit.insightsociety.org/index.php?option=com_content&view=article&id=9&Itemid=1&article_id=24},
   doi = {10.18517/ijaseit.1.1.24}
}

EndNote

%A Al-Tememy, Waleed K. M.
%A Al-Ani, Ibraem A.
%A Al-Ani, Mohammed Q.
%A Al-Alusi, Nadia T.
%A Ismail, Salmah
%D 2011
%T Isolation of Lactobacillus salivarius from Children and Purification of Bacteriocin to Inhibition Cancer Cell in Vitro
%B 2011
%9 Lactobacillus salivarius; Bacteriocins; ion-exchange; tumor cell line
%! Isolation of Lactobacillus salivarius from Children and Purification of Bacteriocin to Inhibition Cancer Cell in Vitro
%K Lactobacillus salivarius; Bacteriocins; ion-exchange; tumor cell line
%X Bacteria being used to make anticancer agents could provide an extra source of lead compounds for the pharmaceutical industry.  Bacterium Lactobacillus salivarius produce compounds that selectively inhibit growth of human cancer cells Lactobacillus salivarius naturally produces a compound called Bacteriocins.  Bacteriocins are bacterial proteins produced to prevent the growth of competing microorganisms in a particular biological niche and we can use it as antineoplastic. The aim of this study was to isolate bacteriocin produced by lactic acid bacteria. A preparation of bacteriocin from a strain Lactobacillus salivarius has long been shown to have antineoplastic activity against a variety of human tumor and animal tumor cell lines in vitro. A total of 60 LAB  were isolated from children stool 45 isolate showed a clear antimicrobial activity against indicator strain Streptococcus aureus and by used sodium phosphate buffer (pH8) from an 80% ammonium sulfate precipitate. The inhibition  activity was determent by well diffusion assay method technique, Bacteriocin purification processes were carried out by using ion-exchange (Trisacryl SP) and gel filtration chromatography (Sephacryl – S300). The apparent molecular mass of partially purified bacteriocin was 15. 848 kDa,  Cell Culture was maintained in RPMI 1640 medium supplemented with 10% (vol/vol) fetal calf serum,  Cytotoxicity of bacteriocin was assessed on human cell line (RD) and animal cell line (MDCK) cell viability after incubation for 48 h in medium containing 500AU/ml (1.15 mg/ml). Both cell types used in this study were sensitive to bacteriocin and the bacteriocin appeared to inhibit proliferation of tumor cell line. The animal cell line was more sensitivity than human cell line.
%U http://ijaseit.insightsociety.org/index.php?option=com_content&view=article&id=9&Itemid=1&article_id=24
%R doi:10.18517/ijaseit.1.1.24
%J International Journal on Advanced Science, Engineering and Information Technology
%V 1
%N 1
%@ 2088-5334

IEEE

Waleed K. M. Al-Tememy,Ibraem A. Al-Ani,Mohammed Q. Al-Ani,Nadia T. Al-Alusi and Salmah Ismail,"Isolation of Lactobacillus salivarius from Children and Purification of Bacteriocin to Inhibition Cancer Cell in Vitro," International Journal on Advanced Science, Engineering and Information Technology, vol. 1, no. 1, pp. 104-108, 2011. [Online]. Available: http://dx.doi.org/10.18517/ijaseit.1.1.24.

RefMan/ProCite (RIS)

TY  - JOUR
AU  - Al-Tememy, Waleed K. M.
AU  - Al-Ani, Ibraem A.
AU  - Al-Ani, Mohammed Q.
AU  - Al-Alusi, Nadia T.
AU  - Ismail, Salmah
PY  - 2011
TI  - Isolation of Lactobacillus salivarius from Children and Purification of Bacteriocin to Inhibition Cancer Cell in Vitro
JF  - International Journal on Advanced Science, Engineering and Information Technology; Vol. 1 (2011) No. 1
Y2  - 2011
SP  - 104
EP  - 108
SN  - 2088-5334
PB  - INSIGHT - Indonesian Society for Knowledge and Human Development
KW  - Lactobacillus salivarius; Bacteriocins; ion-exchange; tumor cell line
N2  - Bacteria being used to make anticancer agents could provide an extra source of lead compounds for the pharmaceutical industry.  Bacterium Lactobacillus salivarius produce compounds that selectively inhibit growth of human cancer cells Lactobacillus salivarius naturally produces a compound called Bacteriocins.  Bacteriocins are bacterial proteins produced to prevent the growth of competing microorganisms in a particular biological niche and we can use it as antineoplastic. The aim of this study was to isolate bacteriocin produced by lactic acid bacteria. A preparation of bacteriocin from a strain Lactobacillus salivarius has long been shown to have antineoplastic activity against a variety of human tumor and animal tumor cell lines in vitro. A total of 60 LAB  were isolated from children stool 45 isolate showed a clear antimicrobial activity against indicator strain Streptococcus aureus and by used sodium phosphate buffer (pH8) from an 80% ammonium sulfate precipitate. The inhibition  activity was determent by well diffusion assay method technique, Bacteriocin purification processes were carried out by using ion-exchange (Trisacryl SP) and gel filtration chromatography (Sephacryl – S300). The apparent molecular mass of partially purified bacteriocin was 15. 848 kDa,  Cell Culture was maintained in RPMI 1640 medium supplemented with 10% (vol/vol) fetal calf serum,  Cytotoxicity of bacteriocin was assessed on human cell line (RD) and animal cell line (MDCK) cell viability after incubation for 48 h in medium containing 500AU/ml (1.15 mg/ml). Both cell types used in this study were sensitive to bacteriocin and the bacteriocin appeared to inhibit proliferation of tumor cell line. The animal cell line was more sensitivity than human cell line.
UR  - http://ijaseit.insightsociety.org/index.php?option=com_content&view=article&id=9&Itemid=1&article_id=24
DO  - 10.18517/ijaseit.1.1.24

RefWorks

RT Journal Article
ID 24
A1 Al-Tememy, Waleed K. M.
A1 Al-Ani, Ibraem A.
A1 Al-Ani, Mohammed Q.
A1 Al-Alusi, Nadia T.
A1 Ismail, Salmah
T1 Isolation of Lactobacillus salivarius from Children and Purification of Bacteriocin to Inhibition Cancer Cell in Vitro
JF International Journal on Advanced Science, Engineering and Information Technology
VO 1
IS 1
YR 2011
SP 104
OP 108
SN 2088-5334
PB INSIGHT - Indonesian Society for Knowledge and Human Development
K1 Lactobacillus salivarius; Bacteriocins; ion-exchange; tumor cell line
AB Bacteria being used to make anticancer agents could provide an extra source of lead compounds for the pharmaceutical industry.  Bacterium Lactobacillus salivarius produce compounds that selectively inhibit growth of human cancer cells Lactobacillus salivarius naturally produces a compound called Bacteriocins.  Bacteriocins are bacterial proteins produced to prevent the growth of competing microorganisms in a particular biological niche and we can use it as antineoplastic. The aim of this study was to isolate bacteriocin produced by lactic acid bacteria. A preparation of bacteriocin from a strain Lactobacillus salivarius has long been shown to have antineoplastic activity against a variety of human tumor and animal tumor cell lines in vitro. A total of 60 LAB  were isolated from children stool 45 isolate showed a clear antimicrobial activity against indicator strain Streptococcus aureus and by used sodium phosphate buffer (pH8) from an 80% ammonium sulfate precipitate. The inhibition  activity was determent by well diffusion assay method technique, Bacteriocin purification processes were carried out by using ion-exchange (Trisacryl SP) and gel filtration chromatography (Sephacryl – S300). The apparent molecular mass of partially purified bacteriocin was 15. 848 kDa,  Cell Culture was maintained in RPMI 1640 medium supplemented with 10% (vol/vol) fetal calf serum,  Cytotoxicity of bacteriocin was assessed on human cell line (RD) and animal cell line (MDCK) cell viability after incubation for 48 h in medium containing 500AU/ml (1.15 mg/ml). Both cell types used in this study were sensitive to bacteriocin and the bacteriocin appeared to inhibit proliferation of tumor cell line. The animal cell line was more sensitivity than human cell line.
LK http://ijaseit.insightsociety.org/index.php?option=com_content&view=article&id=9&Itemid=1&article_id=24
DO  - 10.18517/ijaseit.1.1.24