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Isolation and Identification of Glucoamylase Producer Fungus from Sago Hampas

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@article{IJASEIT337,
   author = {Alfi Asben and Tun Tedja Irawadi},
   title = {Isolation and Identification of Glucoamylase Producer Fungus from Sago Hampas},
   journal = {International Journal on Advanced Science, Engineering and Information Technology},
   volume = {3},
   number = {5},
   year = {2013},
   pages = {330--334},
   keywords = {Sago hampas; isolation; Gliocladium KE; glucoamylase},
   abstract = {Waste of sago processing, notably hampas (ela) still contains sago starch is waste that has not been utilized optimally yet  and causing pollution. Isolation and identification of glucoamylase producer fungus of sago hampas waste  were aims to obtain isolates that have gluco-amylolytic properties, and to know glucoamylase activity of selected fungus isolates after grown on artificial medium.  Indegeneous isolates that can produced glucoamylase will be use to get sugar hidrolysate from starch of sago hampas waste for bioetanol production. The study was conducted with the following stages: 1)Take the sample from the tennis, 2) Isolation and Identification, 3) Characterization (clear zone), and 4) The production of glucoamylase from selected isolates, The results obtained are: 1) Isolation of fungi gluco-amylolytic from 2 sources sago hampas were produced 10 isolates. Ten isolates were divided into 4 genuses: Gliocladium (as dominant isolate), Aspergilus, Rizhopus and Geotrichum. Isolates of Gliocladium KE gaves the largest degradation of starch on PDA-Starch medium (clear zone), and followed by isolates of Aspergillus GA; 2) Production of glucoamylase on sago hampas with modificated Danial medium (1992) gave the highest activity of Gliocladium KE   on the fifth day of incubation, namely: 10.72 U / mL of crude enzyme   from the supernatant of fermentation substrate (S), and 17.16 U / mL for crude enzyme from the extract of isopropanol isolation (E).},
   issn = {2088-5334},
   publisher = {INSIGHT - Indonesian Society for Knowledge and Human Development},
   url = {http://ijaseit.insightsociety.org/index.php?option=com_content&view=article&id=9&Itemid=1&article_id=337},
   doi = {10.18517/ijaseit.3.5.337}
}

EndNote

%A Asben, Alfi
%A Tedja Irawadi, Tun
%D 2013
%T Isolation and Identification of Glucoamylase Producer Fungus from Sago Hampas
%B 2013
%9 Sago hampas; isolation; Gliocladium KE; glucoamylase
%! Isolation and Identification of Glucoamylase Producer Fungus from Sago Hampas
%K Sago hampas; isolation; Gliocladium KE; glucoamylase
%X Waste of sago processing, notably hampas (ela) still contains sago starch is waste that has not been utilized optimally yet  and causing pollution. Isolation and identification of glucoamylase producer fungus of sago hampas waste  were aims to obtain isolates that have gluco-amylolytic properties, and to know glucoamylase activity of selected fungus isolates after grown on artificial medium.  Indegeneous isolates that can produced glucoamylase will be use to get sugar hidrolysate from starch of sago hampas waste for bioetanol production. The study was conducted with the following stages: 1)Take the sample from the tennis, 2) Isolation and Identification, 3) Characterization (clear zone), and 4) The production of glucoamylase from selected isolates, The results obtained are: 1) Isolation of fungi gluco-amylolytic from 2 sources sago hampas were produced 10 isolates. Ten isolates were divided into 4 genuses: Gliocladium (as dominant isolate), Aspergilus, Rizhopus and Geotrichum. Isolates of Gliocladium KE gaves the largest degradation of starch on PDA-Starch medium (clear zone), and followed by isolates of Aspergillus GA; 2) Production of glucoamylase on sago hampas with modificated Danial medium (1992) gave the highest activity of Gliocladium KE   on the fifth day of incubation, namely: 10.72 U / mL of crude enzyme   from the supernatant of fermentation substrate (S), and 17.16 U / mL for crude enzyme from the extract of isopropanol isolation (E).
%U http://ijaseit.insightsociety.org/index.php?option=com_content&view=article&id=9&Itemid=1&article_id=337
%R doi:10.18517/ijaseit.3.5.337
%J International Journal on Advanced Science, Engineering and Information Technology
%V 3
%N 5
%@ 2088-5334

IEEE

Alfi Asben and Tun Tedja Irawadi,"Isolation and Identification of Glucoamylase Producer Fungus from Sago Hampas," International Journal on Advanced Science, Engineering and Information Technology, vol. 3, no. 5, pp. 330-334, 2013. [Online]. Available: http://dx.doi.org/10.18517/ijaseit.3.5.337.

RefMan/ProCite (RIS)

TY  - JOUR
AU  - Asben, Alfi
AU  - Tedja Irawadi, Tun
PY  - 2013
TI  - Isolation and Identification of Glucoamylase Producer Fungus from Sago Hampas
JF  - International Journal on Advanced Science, Engineering and Information Technology; Vol. 3 (2013) No. 5
Y2  - 2013
SP  - 330
EP  - 334
SN  - 2088-5334
PB  - INSIGHT - Indonesian Society for Knowledge and Human Development
KW  - Sago hampas; isolation; Gliocladium KE; glucoamylase
N2  - Waste of sago processing, notably hampas (ela) still contains sago starch is waste that has not been utilized optimally yet  and causing pollution. Isolation and identification of glucoamylase producer fungus of sago hampas waste  were aims to obtain isolates that have gluco-amylolytic properties, and to know glucoamylase activity of selected fungus isolates after grown on artificial medium.  Indegeneous isolates that can produced glucoamylase will be use to get sugar hidrolysate from starch of sago hampas waste for bioetanol production. The study was conducted with the following stages: 1)Take the sample from the tennis, 2) Isolation and Identification, 3) Characterization (clear zone), and 4) The production of glucoamylase from selected isolates, The results obtained are: 1) Isolation of fungi gluco-amylolytic from 2 sources sago hampas were produced 10 isolates. Ten isolates were divided into 4 genuses: Gliocladium (as dominant isolate), Aspergilus, Rizhopus and Geotrichum. Isolates of Gliocladium KE gaves the largest degradation of starch on PDA-Starch medium (clear zone), and followed by isolates of Aspergillus GA; 2) Production of glucoamylase on sago hampas with modificated Danial medium (1992) gave the highest activity of Gliocladium KE   on the fifth day of incubation, namely: 10.72 U / mL of crude enzyme   from the supernatant of fermentation substrate (S), and 17.16 U / mL for crude enzyme from the extract of isopropanol isolation (E).
UR  - http://ijaseit.insightsociety.org/index.php?option=com_content&view=article&id=9&Itemid=1&article_id=337
DO  - 10.18517/ijaseit.3.5.337

RefWorks

RT Journal Article
ID 337
A1 Asben, Alfi
A1 Tedja Irawadi, Tun
T1 Isolation and Identification of Glucoamylase Producer Fungus from Sago Hampas
JF International Journal on Advanced Science, Engineering and Information Technology
VO 3
IS 5
YR 2013
SP 330
OP 334
SN 2088-5334
PB INSIGHT - Indonesian Society for Knowledge and Human Development
K1 Sago hampas; isolation; Gliocladium KE; glucoamylase
AB Waste of sago processing, notably hampas (ela) still contains sago starch is waste that has not been utilized optimally yet  and causing pollution. Isolation and identification of glucoamylase producer fungus of sago hampas waste  were aims to obtain isolates that have gluco-amylolytic properties, and to know glucoamylase activity of selected fungus isolates after grown on artificial medium.  Indegeneous isolates that can produced glucoamylase will be use to get sugar hidrolysate from starch of sago hampas waste for bioetanol production. The study was conducted with the following stages: 1)Take the sample from the tennis, 2) Isolation and Identification, 3) Characterization (clear zone), and 4) The production of glucoamylase from selected isolates, The results obtained are: 1) Isolation of fungi gluco-amylolytic from 2 sources sago hampas were produced 10 isolates. Ten isolates were divided into 4 genuses: Gliocladium (as dominant isolate), Aspergilus, Rizhopus and Geotrichum. Isolates of Gliocladium KE gaves the largest degradation of starch on PDA-Starch medium (clear zone), and followed by isolates of Aspergillus GA; 2) Production of glucoamylase on sago hampas with modificated Danial medium (1992) gave the highest activity of Gliocladium KE   on the fifth day of incubation, namely: 10.72 U / mL of crude enzyme   from the supernatant of fermentation substrate (S), and 17.16 U / mL for crude enzyme from the extract of isopropanol isolation (E).
LK http://ijaseit.insightsociety.org/index.php?option=com_content&view=article&id=9&Itemid=1&article_id=337
DO  - 10.18517/ijaseit.3.5.337