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Saccharification Waste Biomass Rice Straw IR-64 by Using Xylanase from Indigenous Marine Bacteria Bacillus safensis LBF-002

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@article{IJASEIT558,
   author = {Apridah Cameliawati Djohan and Urip Perwitasari and Yopi Yopi},
   title = {Saccharification Waste Biomass Rice Straw IR-64 by Using Xylanase from Indigenous Marine Bacteria Bacillus safensis LBF-002},
   journal = {International Journal on Advanced Science, Engineering and Information Technology},
   volume = {6},
   number = {1},
   year = {2016},
   pages = {40--44},
   keywords = {Saccharification; Xylanase; Rice straw; Bacillus safensis LBF-002; Xylooligosaccharide.},
   abstract = {

Abstract— Agricultural residues have an enormous potential as renewable carbon and energy sources. Saccharification of agricultural by microbial hydrolytic enzymes is the first step of bioconversion of organic material into reducing sugar. The main purpose of this research is converting rice straw IR-64 waste biomass into reducing sugar xylo-oligosaccharides by using xylanase from indigenous marine bacteria Bacillus safensis LBF-002. The optimation of xylanase in rice straw medium are using stability of pH and temperature were resulted higher activity in pH 7 and 50 °C which result 2.228 U/mL in 24 h incubation. The xylanase was concentrated with PEG 6000 in ratio (1:1) become 16.578 U/mL and was used for hydrolyze the rice straw for getting the sugar reduction product. The sugar reduction component from rice straw saccharification was analyzed using Thin Layer Chromatography and also High Performance Liquid Chromatography (HPLC). The present study was a first effort to explore marine bacteria Bacillus safensis LBF-002 to produce and obtain the optimum condition for producing xylooligosaccharide from rice straw waste. The best result for hydrolysis experiment showed in saccharafication with 2.5% rice straw and crude enzyme xylanase 4 U/mL for 1 h and 2 h incubation which is resulted xylose, xylobiose, and xylohexose.

},    issn = {2088-5334},    publisher = {INSIGHT - Indonesian Society for Knowledge and Human Development},    url = {http://ijaseit.insightsociety.org/index.php?option=com_content&view=article&id=9&Itemid=1&article_id=558},    doi = {10.18517/ijaseit.6.1.558} }

EndNote

%A Djohan, Apridah Cameliawati
%A Perwitasari, Urip
%A Yopi, Yopi
%D 2016
%T Saccharification Waste Biomass Rice Straw IR-64 by Using Xylanase from Indigenous Marine Bacteria Bacillus safensis LBF-002
%B 2016
%9 Saccharification; Xylanase; Rice straw; Bacillus safensis LBF-002; Xylooligosaccharide.
%! Saccharification Waste Biomass Rice Straw IR-64 by Using Xylanase from Indigenous Marine Bacteria Bacillus safensis LBF-002
%K Saccharification; Xylanase; Rice straw; Bacillus safensis LBF-002; Xylooligosaccharide.
%X 

Abstract— Agricultural residues have an enormous potential as renewable carbon and energy sources. Saccharification of agricultural by microbial hydrolytic enzymes is the first step of bioconversion of organic material into reducing sugar. The main purpose of this research is converting rice straw IR-64 waste biomass into reducing sugar xylo-oligosaccharides by using xylanase from indigenous marine bacteria Bacillus safensis LBF-002. The optimation of xylanase in rice straw medium are using stability of pH and temperature were resulted higher activity in pH 7 and 50 °C which result 2.228 U/mL in 24 h incubation. The xylanase was concentrated with PEG 6000 in ratio (1:1) become 16.578 U/mL and was used for hydrolyze the rice straw for getting the sugar reduction product. The sugar reduction component from rice straw saccharification was analyzed using Thin Layer Chromatography and also High Performance Liquid Chromatography (HPLC). The present study was a first effort to explore marine bacteria Bacillus safensis LBF-002 to produce and obtain the optimum condition for producing xylooligosaccharide from rice straw waste. The best result for hydrolysis experiment showed in saccharafication with 2.5% rice straw and crude enzyme xylanase 4 U/mL for 1 h and 2 h incubation which is resulted xylose, xylobiose, and xylohexose.

%U http://ijaseit.insightsociety.org/index.php?option=com_content&view=article&id=9&Itemid=1&article_id=558 %R doi:10.18517/ijaseit.6.1.558 %J International Journal on Advanced Science, Engineering and Information Technology %V 6 %N 1 %@ 2088-5334

IEEE

Apridah Cameliawati Djohan,Urip Perwitasari and Yopi Yopi,"Saccharification Waste Biomass Rice Straw IR-64 by Using Xylanase from Indigenous Marine Bacteria Bacillus safensis LBF-002," International Journal on Advanced Science, Engineering and Information Technology, vol. 6, no. 1, pp. 40-44, 2016. [Online]. Available: http://dx.doi.org/10.18517/ijaseit.6.1.558.

RefMan/ProCite (RIS)

TY  - JOUR
AU  - Djohan, Apridah Cameliawati
AU  - Perwitasari, Urip
AU  - Yopi, Yopi
PY  - 2016
TI  - Saccharification Waste Biomass Rice Straw IR-64 by Using Xylanase from Indigenous Marine Bacteria Bacillus safensis LBF-002
JF  - International Journal on Advanced Science, Engineering and Information Technology; Vol. 6 (2016) No. 1
Y2  - 2016
SP  - 40
EP  - 44
SN  - 2088-5334
PB  - INSIGHT - Indonesian Society for Knowledge and Human Development
KW  - Saccharification; Xylanase; Rice straw; Bacillus safensis LBF-002; Xylooligosaccharide.
N2  - 

Abstract— Agricultural residues have an enormous potential as renewable carbon and energy sources. Saccharification of agricultural by microbial hydrolytic enzymes is the first step of bioconversion of organic material into reducing sugar. The main purpose of this research is converting rice straw IR-64 waste biomass into reducing sugar xylo-oligosaccharides by using xylanase from indigenous marine bacteria Bacillus safensis LBF-002. The optimation of xylanase in rice straw medium are using stability of pH and temperature were resulted higher activity in pH 7 and 50 °C which result 2.228 U/mL in 24 h incubation. The xylanase was concentrated with PEG 6000 in ratio (1:1) become 16.578 U/mL and was used for hydrolyze the rice straw for getting the sugar reduction product. The sugar reduction component from rice straw saccharification was analyzed using Thin Layer Chromatography and also High Performance Liquid Chromatography (HPLC). The present study was a first effort to explore marine bacteria Bacillus safensis LBF-002 to produce and obtain the optimum condition for producing xylooligosaccharide from rice straw waste. The best result for hydrolysis experiment showed in saccharafication with 2.5% rice straw and crude enzyme xylanase 4 U/mL for 1 h and 2 h incubation which is resulted xylose, xylobiose, and xylohexose.

UR - http://ijaseit.insightsociety.org/index.php?option=com_content&view=article&id=9&Itemid=1&article_id=558 DO - 10.18517/ijaseit.6.1.558

RefWorks

RT Journal Article
ID 558
A1 Djohan, Apridah Cameliawati
A1 Perwitasari, Urip
A1 Yopi, Yopi
T1 Saccharification Waste Biomass Rice Straw IR-64 by Using Xylanase from Indigenous Marine Bacteria Bacillus safensis LBF-002
JF International Journal on Advanced Science, Engineering and Information Technology
VO 6
IS 1
YR 2016
SP 40
OP 44
SN 2088-5334
PB INSIGHT - Indonesian Society for Knowledge and Human Development
K1 Saccharification; Xylanase; Rice straw; Bacillus safensis LBF-002; Xylooligosaccharide.
AB 

Abstract— Agricultural residues have an enormous potential as renewable carbon and energy sources. Saccharification of agricultural by microbial hydrolytic enzymes is the first step of bioconversion of organic material into reducing sugar. The main purpose of this research is converting rice straw IR-64 waste biomass into reducing sugar xylo-oligosaccharides by using xylanase from indigenous marine bacteria Bacillus safensis LBF-002. The optimation of xylanase in rice straw medium are using stability of pH and temperature were resulted higher activity in pH 7 and 50 °C which result 2.228 U/mL in 24 h incubation. The xylanase was concentrated with PEG 6000 in ratio (1:1) become 16.578 U/mL and was used for hydrolyze the rice straw for getting the sugar reduction product. The sugar reduction component from rice straw saccharification was analyzed using Thin Layer Chromatography and also High Performance Liquid Chromatography (HPLC). The present study was a first effort to explore marine bacteria Bacillus safensis LBF-002 to produce and obtain the optimum condition for producing xylooligosaccharide from rice straw waste. The best result for hydrolysis experiment showed in saccharafication with 2.5% rice straw and crude enzyme xylanase 4 U/mL for 1 h and 2 h incubation which is resulted xylose, xylobiose, and xylohexose.

LK http://ijaseit.insightsociety.org/index.php?option=com_content&view=article&id=9&Itemid=1&article_id=558 DO - 10.18517/ijaseit.6.1.558