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In Vitro Regeneration of Sugar Palm (Arenga pinnata Wurmb Merr.)

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@article{IJASEIT8433,
   author = {Nazatul Asikin Muda and Asmah Awal and Mohd Yusoff Abdullah and Nor Azma Yusuf and Nor Azma Yusuf},
   title = {In Vitro Regeneration of Sugar Palm (Arenga pinnata Wurmb Merr.)},
   journal = {International Journal on Advanced Science, Engineering and Information Technology},
   volume = {9},
   number = {3},
   year = {2019},
   pages = {888--894},
   keywords = {acclimatization; basal stem explant; light intensity; plant growth regulators (PGRs).},
   abstract = {Optimization of a protocol for in vitro regeneration of sugar palm (Arenga pinnata Wurmb Merr.) by direct organogenesis using the immature zygotic embryo and basal stem explants of in vitro raised seedlings has been developed. The explants were cultured on MS medium supplemented with different concentrations of PGRs, and the organogenic capability of the explants was investigated. Both explants were responsive, but the results were greatly dependent on the genotype and the culture medium composition investigated. After 8 weeks of culture, basal stem explant cultured on MS + 1.0 mg/L Kin + 2.0 mg/L NAA promoted optimum response for direct organogenesis (90%) with a total of 9 adventitious shoots and 25 roots. Subculturing of individual shoots in basal MS medium (MS0) optimized further development. Meanwhile, immature zygotic embryo explant cultured on MS + 2.0 mg/L BAP + 2.0 mg/L NAA promoted optimum root regeneration (70%), though MS medium containing 2.0 mg/L BAP + 1.0 mg/L GA3 and 1.0 mg/L AgNO3 promoted optimum shoots growth (total of 10 shoots) at the rate of 0.04%. In vitro rooting of the regenerated shoots was successfully obtained on MS + 3.0 mg/L IBA. Established in vitro plantlets with roots were hardened in soil: peat moss: perlite (2:2:1) mixture. Acclimatization of plantlets was successfully obtained with 70% seedlings survived after 4 month’s exposures under the light intensity of 50-100 µmol m-2s-1.},
   issn = {2088-5334},
   publisher = {INSIGHT - Indonesian Society for Knowledge and Human Development},
   url = {http://ijaseit.insightsociety.org/index.php?option=com_content&view=article&id=9&Itemid=1&article_id=8433},
   doi = {10.18517/ijaseit.9.3.8433}
}

EndNote

%A Muda, Nazatul Asikin
%A Awal, Asmah
%A Abdullah, Mohd Yusoff
%A Yusuf, Nor Azma
%A Yusuf, Nor Azma
%D 2019
%T In Vitro Regeneration of Sugar Palm (Arenga pinnata Wurmb Merr.)
%B 2019
%9 acclimatization; basal stem explant; light intensity; plant growth regulators (PGRs).
%! In Vitro Regeneration of Sugar Palm (Arenga pinnata Wurmb Merr.)
%K acclimatization; basal stem explant; light intensity; plant growth regulators (PGRs).
%X Optimization of a protocol for in vitro regeneration of sugar palm (Arenga pinnata Wurmb Merr.) by direct organogenesis using the immature zygotic embryo and basal stem explants of in vitro raised seedlings has been developed. The explants were cultured on MS medium supplemented with different concentrations of PGRs, and the organogenic capability of the explants was investigated. Both explants were responsive, but the results were greatly dependent on the genotype and the culture medium composition investigated. After 8 weeks of culture, basal stem explant cultured on MS + 1.0 mg/L Kin + 2.0 mg/L NAA promoted optimum response for direct organogenesis (90%) with a total of 9 adventitious shoots and 25 roots. Subculturing of individual shoots in basal MS medium (MS0) optimized further development. Meanwhile, immature zygotic embryo explant cultured on MS + 2.0 mg/L BAP + 2.0 mg/L NAA promoted optimum root regeneration (70%), though MS medium containing 2.0 mg/L BAP + 1.0 mg/L GA3 and 1.0 mg/L AgNO3 promoted optimum shoots growth (total of 10 shoots) at the rate of 0.04%. In vitro rooting of the regenerated shoots was successfully obtained on MS + 3.0 mg/L IBA. Established in vitro plantlets with roots were hardened in soil: peat moss: perlite (2:2:1) mixture. Acclimatization of plantlets was successfully obtained with 70% seedlings survived after 4 month’s exposures under the light intensity of 50-100 µmol m-2s-1.
%U http://ijaseit.insightsociety.org/index.php?option=com_content&view=article&id=9&Itemid=1&article_id=8433
%R doi:10.18517/ijaseit.9.3.8433
%J International Journal on Advanced Science, Engineering and Information Technology
%V 9
%N 3
%@ 2088-5334

IEEE

Nazatul Asikin Muda,Asmah Awal,Mohd Yusoff Abdullah,Nor Azma Yusuf and Nor Azma Yusuf,"In Vitro Regeneration of Sugar Palm (Arenga pinnata Wurmb Merr.)," International Journal on Advanced Science, Engineering and Information Technology, vol. 9, no. 3, pp. 888-894, 2019. [Online]. Available: http://dx.doi.org/10.18517/ijaseit.9.3.8433.

RefMan/ProCite (RIS)

TY  - JOUR
AU  - Muda, Nazatul Asikin
AU  - Awal, Asmah
AU  - Abdullah, Mohd Yusoff
AU  - Yusuf, Nor Azma
AU  - Yusuf, Nor Azma
PY  - 2019
TI  - In Vitro Regeneration of Sugar Palm (Arenga pinnata Wurmb Merr.)
JF  - International Journal on Advanced Science, Engineering and Information Technology; Vol. 9 (2019) No. 3
Y2  - 2019
SP  - 888
EP  - 894
SN  - 2088-5334
PB  - INSIGHT - Indonesian Society for Knowledge and Human Development
KW  - acclimatization; basal stem explant; light intensity; plant growth regulators (PGRs).
N2  - Optimization of a protocol for in vitro regeneration of sugar palm (Arenga pinnata Wurmb Merr.) by direct organogenesis using the immature zygotic embryo and basal stem explants of in vitro raised seedlings has been developed. The explants were cultured on MS medium supplemented with different concentrations of PGRs, and the organogenic capability of the explants was investigated. Both explants were responsive, but the results were greatly dependent on the genotype and the culture medium composition investigated. After 8 weeks of culture, basal stem explant cultured on MS + 1.0 mg/L Kin + 2.0 mg/L NAA promoted optimum response for direct organogenesis (90%) with a total of 9 adventitious shoots and 25 roots. Subculturing of individual shoots in basal MS medium (MS0) optimized further development. Meanwhile, immature zygotic embryo explant cultured on MS + 2.0 mg/L BAP + 2.0 mg/L NAA promoted optimum root regeneration (70%), though MS medium containing 2.0 mg/L BAP + 1.0 mg/L GA3 and 1.0 mg/L AgNO3 promoted optimum shoots growth (total of 10 shoots) at the rate of 0.04%. In vitro rooting of the regenerated shoots was successfully obtained on MS + 3.0 mg/L IBA. Established in vitro plantlets with roots were hardened in soil: peat moss: perlite (2:2:1) mixture. Acclimatization of plantlets was successfully obtained with 70% seedlings survived after 4 month’s exposures under the light intensity of 50-100 µmol m-2s-1.
UR  - http://ijaseit.insightsociety.org/index.php?option=com_content&view=article&id=9&Itemid=1&article_id=8433
DO  - 10.18517/ijaseit.9.3.8433

RefWorks

RT Journal Article
ID 8433
A1 Muda, Nazatul Asikin
A1 Awal, Asmah
A1 Abdullah, Mohd Yusoff
A1 Yusuf, Nor Azma
A1 Yusuf, Nor Azma
T1 In Vitro Regeneration of Sugar Palm (Arenga pinnata Wurmb Merr.)
JF International Journal on Advanced Science, Engineering and Information Technology
VO 9
IS 3
YR 2019
SP 888
OP 894
SN 2088-5334
PB INSIGHT - Indonesian Society for Knowledge and Human Development
K1 acclimatization; basal stem explant; light intensity; plant growth regulators (PGRs).
AB Optimization of a protocol for in vitro regeneration of sugar palm (Arenga pinnata Wurmb Merr.) by direct organogenesis using the immature zygotic embryo and basal stem explants of in vitro raised seedlings has been developed. The explants were cultured on MS medium supplemented with different concentrations of PGRs, and the organogenic capability of the explants was investigated. Both explants were responsive, but the results were greatly dependent on the genotype and the culture medium composition investigated. After 8 weeks of culture, basal stem explant cultured on MS + 1.0 mg/L Kin + 2.0 mg/L NAA promoted optimum response for direct organogenesis (90%) with a total of 9 adventitious shoots and 25 roots. Subculturing of individual shoots in basal MS medium (MS0) optimized further development. Meanwhile, immature zygotic embryo explant cultured on MS + 2.0 mg/L BAP + 2.0 mg/L NAA promoted optimum root regeneration (70%), though MS medium containing 2.0 mg/L BAP + 1.0 mg/L GA3 and 1.0 mg/L AgNO3 promoted optimum shoots growth (total of 10 shoots) at the rate of 0.04%. In vitro rooting of the regenerated shoots was successfully obtained on MS + 3.0 mg/L IBA. Established in vitro plantlets with roots were hardened in soil: peat moss: perlite (2:2:1) mixture. Acclimatization of plantlets was successfully obtained with 70% seedlings survived after 4 month’s exposures under the light intensity of 50-100 µmol m-2s-1.
LK http://ijaseit.insightsociety.org/index.php?option=com_content&view=article&id=9&Itemid=1&article_id=8433
DO  - 10.18517/ijaseit.9.3.8433