International Journal on Advanced Science, Engineering and Information Technology, Vol. 12 (2022) No. 3, pages: 946-952, DOI:10.18517/ijaseit.12.3.15710

Extraction of Phenolic Compounds from Coleus amboinicus Leaves by Microwave-assisted Extraction: Optimization of the Operating Condition

Ni'matul Izza, Nani Sumarni, Niken Dieni Pramesi, Ucha Aditya Mahardi, Shinta Rosalia Dewi

Abstract

Torbangun (Coleus amboinicus L) leaf is one of the herbs from Indonesia that has been reported to have many bioactive compounds for human health. These phytochemicals are predominantly incorporated into phenolics, such as flavonoids. An extraction process is needed to obtain all the valuable compounds, and conventional solvent extraction, such as heating and maceration, could be easily applied because of its simplicity. However, this method requires high energy, a longer extraction time, and a large amount of solvent. To overcome these limitations, in this study, microwave-assisted extraction (MAE) was utilized to extract phenolic compounds, including flavonoids from torbangun leaves. Three factors (solvent to feed ratio, microwave power, and extraction time) were evaluated to affect the three responses (extract volume, TPC, and TFC). The extraction process optimization was carried out to get a higher response. The Box-Behnken experiment design and Response Surface Methodology (RSM) determined the optimal operating condition. The result shows 30 ml/g of solvent to feed ratio, 300 W of microwave power, and 4.6 minutes of extraction time as the optimum condition. Applying those conditions, the extract is expected to reach 10.13 ml of extract volume, 9.17 mg GAE/ ml extract of total phenolic, and 3.62 mg QE/ ml extract of total flavonoid. In sum, MAE was useful for extracting phenolic compounds from torbangun leaves and could be an alternative method due to its high efficiency.

Keywords:

Coleous amboinicus; Torbangun; MAE; TPC; TFC; RSM

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