International Journal on Advanced Science, Engineering and Information Technology, Vol. 3 (2013) No. 5, pages: 330-334, DOI:10.18517/ijaseit.3.5.337

Isolation and Identification of Glucoamylase Producer Fungus from Sago Hampas

Alfi Asben, Tun Tedja Irawadi

Abstract

Waste of sago processing, notably hampas (ela) still contains sago starch is waste that has not been utilized optimally yet  and causing pollution. Isolation and identification of glucoamylase producer fungus of sago hampas waste  were aims to obtain isolates that have gluco-amylolytic properties, and to know glucoamylase activity of selected fungus isolates after grown on artificial medium.  Indegeneous isolates that can produced glucoamylase will be use to get sugar hidrolysate from starch of sago hampas waste for bioetanol production. The study was conducted with the following stages: 1)Take the sample from the tennis, 2) Isolation and Identification, 3) Characterization (clear zone), and 4) The production of glucoamylase from selected isolates, The results obtained are: 1) Isolation of fungi gluco-amylolytic from 2 sources sago hampas were produced 10 isolates. Ten isolates were divided into 4 genuses: Gliocladium (as dominant isolate), Aspergilus, Rizhopus and Geotrichum. Isolates of Gliocladium KE gaves the largest degradation of starch on PDA-Starch medium (clear zone), and followed by isolates of Aspergillus GA; 2) Production of glucoamylase on sago hampas with modificated Danial medium (1992) gave the highest activity of Gliocladium KE   on the fifth day of incubation, namely: 10.72 U / mL of crude enzyme   from the supernatant of fermentation substrate (S), and 17.16 U / mL for crude enzyme from the extract of isopropanol isolation (E).

Keywords:

Sago hampas; isolation; Gliocladium KE; glucoamylase

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