International Journal on Advanced Science, Engineering and Information Technology, Vol. 8 (2018) No. 6, DOI:10.18517/ijaseit.8.6.4196

Fungal Production of Xylanase from Oil Palm Empty Fruit Bunches via Solid State Fermentation

Efri Mardawati

Abstract

The abundance of Oil Palm Empty Fruit Bunches (EFB), the lignocellulosic biomass waste from Crude Palm Oil (CPO) industries, offers a potential for its application as raw material for biorefinery. Its xylan content, in particular, suggest that EFB can be used as the raw material for xylanase production. This manuscript studies xylanasse production by fungal Trichoderma viride ITB CC L67, Aspergillus niger ITB CC L51, and Penicillium sp. ITB CC L96 using EFB as the substrate in solid state fermentation. Response surface methodology (RSM) is applied to determine the optimal condistion operation of each significant variable, including temperature and substrate ratio (g EFB in mL liquid medium). The central composite design (CCD) is employed to investigate the individual crucial component of the condition operation, which is significantly affected the enzyme activity. The highest obtained xylanase activity is 740.6 U/mL or 5,095.5 U/g substrate, produced by T.viride ITB CC L.67 at 32.8oC and substrate ratio 0.63.

Keywords:

Oil Palm Empty Fruit Bunches (EFB); optimation; solid state fermentation; xylanase; xylose

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