International Journal on Advanced Science, Engineering and Information Technology, Vol. 9 (2019) No. 2, pages: 390-396, DOI:10.18517/ijaseit.9.2.4938

Isolates of Lipolytic, Proteolytic and Cellulolytic Bacteria from Palm Oil Mill Effluent and Their Potency as Consortium

Muhammad Said, Muhammad Faizal, Bambang Yudono, - Hasanudin, Sriwi Pertiwi Estuningsih

Abstract

Study of lipolytic, proteolytic and cellulolytic bacteria in variety, especially the study of isolation technique to identify, cultivation technique to produce many researchers have done enzyme and activity test.  While the study on lipolytic, proteolytic and cellulolytic that support the consortium bacteria having the capability of degrading fats, protein and cellulose being the wastewater components have not been conducted yet. This is a novelty of this study.  This study aims to obtain consortium of bacteria having lipolytic, proteolytic and cellulolytic capability from the palm oil mill effluent. The lipolytic, proteolytic and cellulolytic bacteria were isolated from the wastewater using medium mineral (MM), and each of the bacteria was enriched by vegetable oil, casein and Carboxy Methyl Cellulose (CMC). Each of the isolates of bacteria that had been selected was conducted oxygen requirement and synergism tests, and then the consortium of bacteria was wade. Two types of consortium of the bacteria were obtained, that is a facultative anaerobic consortium that consists of two bacteria Bacillus toyonensis strain BCT – 7112 and Stenotrophomonas rhizophila strain e-p10, and the aerobic consortium that consists of three bacteria, namely: Bacillus cereus ATCC 14579, Pseudomonas azotoformans strain NBRC 12693 and Burkholderia cepacia ATCC 25416.

Keywords:

bacteria; lipolytic; proteolytic; cellulolytic; consortium.

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