International Journal on Advanced Science, Engineering and Information Technology, Vol. 5 (2015) No. 2, pages: 107-109, DOI:10.18517/ijaseit.5.2.498

Preliminary Studies of Halophilic Yeasts Antimicrobial Activities Isolated from Cocoa Bean Pulp towards E.coli and Salmonella spp.

Hanna Indah, Fannisa Putri, Gemilang Lara Utama

Abstract

Aims of the research was to determine antimicrobial activities of halophilic yeasts isolated from cocoa bean pulp towards E. coli and Salmonella spp.. Research done with experimental methods and the data were descriptive-exploratory analyzed. Yeasts isolated from cocoa bean pulp (third days fermentation) and cultured on Malt Extract Agar (MEA). Total yeasts population on Nutrient Agar (NA) with addition of 5 and 10% (w/v) NaCl counted to determine potential halophilic yeast. Antimicrobial activities towards E.coli and Salmonella spp. determined by diffusion well method on NA. Isolate C2 shown halophilic characteristic with total yeast population of 9.2 x 102 cfu/g on 5% NaCl and zero yeasts population on 10% NaCl, while isolate C1 shown better halophilic characteristic with total yeast population of 2.1 x 103 cfu/g on 5% NaCl and 1.3 x103 cfu/g on 10% NaCl. Antimicrobial activites of selected isolates shown by isolate C1 with 1-2 mm clear zone towards E.coli and 2-3 mm clear zone towards Salmonella spp. while isolate C2 shown no inhibition towards E.coli and 5-10 mm clear zone inhibition towards Salmonella spp..

Keywords:

Halophilic Yeasts, Cocoa Bean Pulp, Antimicrobial Activities, E.coli, Salmonella spp.

Viewed: 207 times (since Sept 4, 2017)

cite this paper     download