International Journal on Advanced Science, Engineering and Information Technology, Vol. 5 (2015) No. 3, pages: 152-154, DOI:10.18517/ijaseit.5.3.509

Preliminary Identification of Potential Halophilic Bacteria Isolated from ‘Asam Sunti’ – Indonesian Traditional Herbs, in Inhibiting the Growth of E.coli and Salmonella spp.

Fannisa Putri, Hanna Indah, Gemilang Lara Utama

Abstract

The research aimed to determine potential halophilic bacteria isolated from Asam Sunti in inhibiting the growth of E. coli and Salmonella spp.. Research done experimentally and data analyzed by descriptive-exploratory method. Asam Sunti collected from market in Nanggroe Aceh Darussalam were cultured in Nutrient Agar (NA) with addition of 5 and 10% (w/v) NaCl and the total bacteria population was counted. Two different colonies isolated then identified by macroscopic, microscopic and gram staining. Abilities in inhibit the growth of E.coli and Salmonella spp. determined by diffusion well method on NA. Results showed that the best isolate was isolate A1 (rod shaped, gram negative bacteria) that resulting in 1.6 x 105 cfu/ml in 5% NaCl and 1.2 x 105 cfu/ml in 10% NaCl with no inhibition abilities towards E.coli and 11 mm of clear zone inhibition towards Salmonella spp..

Keywords:

Halophilic Bacteria, Asam Sunti, Inhibition, E.coli, Salmonella spp.

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