International Journal on Advanced Science, Engineering and Information Technology, Vol. 9 (2019) No. 4, pages: 1309-1316, DOI:10.18517/ijaseit.9.4.5200

Preliminary Characterization of Crude Lectins Fractions of Red Macroalgae Species Collected from the Southern Coast of Gunungkidul Indonesia

Choirul Anam, Danar Praseptiangga, Nurrahmi Dewi Fajarningsih, Ekowati Chasanah, Ahmad Yunus

Abstract

Lectins or hemagglutininsare specific sugar-binding proteins (glycoproteins) of non-immune origins that typically agglutinate certain cells and/or precipitate glycoconjugates. This study aimed to evaluate the activity of hemagglutination, protein comtent, and initial characterization (the stability towards pH, temperature, divalent cation and inhibition of hemagglutination activity by sugars and/or glycoproteins) crude lectins fractions of red macroalgae from the coast of Gunungkidul Beach, Yogyakarta. The findings showed that hemagglutination activity using trypsin treated rabbit erythrocytes of crude fractions of red algae lectins Nitophylium punctatum species, Helminthora divaricata, and Acanthophora spicifera were 26, 26, and 213, respectively. The protein contents were 8,123.47 μg / ml; 9,545.65 μg / ml; and 7,368.45 μg / ml. Although the three red macroalgae species tend to maintain a relatively stable pH, their temperature tolerance was unstable, and were unaffected by divalent cations. The carbohydrate-binding specificities assay of the red macroalgae species showed the occcurence of inhibition in certain glycoproteins and not simple sugars. 

Keywords:

Red macroalgae; Lectin; Crude fraction; Hemagglutination; Preliminary characterization

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