International Journal on Advanced Science, Engineering and Information Technology, Vol. 8 (2018) No. 4-2: Special Issue on Empowering the Nation via 4IR (The Fourth Industrial Revolution)., pages: 1648-1653, Chief Editor: Khairuddin Omar | Editorial Boards : Shahnorbanun Sahran Hassan, Nor Samsiah Sani, Heuiseok Lim & Danial Hoosyar, DOI:10.18517/ijaseit.8.4-2.7037

Development of Porous PCL Based Microcarrier

Muhammad Amirul N. A. Halim, Yumi Z. H Y. Hashim, Nurhusna Samsudin, Hamzah M. Salleh

Abstract

Polycaprolactone (PCL) has been used for cell cultivation due to its biocompatibility. PCL microcarrier featuring microporous structure were generated in the present study. PCL-based porous microcarrier was fabricated with different parameters by using emulsion evaporation method. From the study, size distribution of the microcarrier and the size of pore generated on microcarrier were affected by most of the parameters tested such as sterring speed, PVA concentration, camphene concentration, temperature and ratio between PCL and solvent.  Optimum pore size was generated at 20% camphene concentration with the average size of 11.74 µm which is conducive for cells to attached and populated well within the pores.  The surface properties of developed porous can be improved for potential application in cell culture research and development, as well as in tissue engineering.

Keywords:

halal; microcarrier; cell culture; porous; polycaprolactone.

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