International Journal on Advanced Science, Engineering and Information Technology, Vol. 6 (2016) No. 2, pages: 201-204, DOI:10.18517/ijaseit.6.2.716

Immobilization of Crown Flower (Calotropis gigantea) Protease Using Celite and Dextrin

Yuli Witono

Abstract

Immobilization of crown flower (Calotropis gigantea) protease using celite and dextrin by entrapment method was investigated in this study. The influenced of celite and dextrin at the concentration (6, 8, 10, 15, and 20 g/g) on enzyme production were evaluated. The optimum condition of immobilized crown flower protease was on temperature 55oC and pH 7.0. Results showed that the best concentration is 8 g/g that reached maximum specific enzyme activity was 5.69 x10-5 U/mg at celite and 3,309.10-6 U/mg at dextrin. Immobilized enzyme is more stable than free enzyme based on the change of pH and temperature of environment. These results indicated that celite is better than dextrin for crown flower protease production.

Keywords:

Crown flower (Calotropis gigantea) protease; immobilization; celite and dextrin; specific activity of enzyme

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