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Hydrolytic and Transglycolation Characteristics of Xanthomonas campestris and Bacillus megaterium in Several Substrates

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@article{IJASEIT12403,
   author = {Heny Herawati and Elmi Kamsiati and - Sunarmani and S Joni Munarso and Ina Supriyatna and Tri Aminingsih},
   title = {Hydrolytic and Transglycolation Characteristics of Xanthomonas campestris and Bacillus megaterium in Several Substrates},
   journal = {International Journal on Advanced Science, Engineering and Information Technology},
   volume = {11},
   number = {1},
   year = {2021},
   pages = {172--178},
   keywords = {Substrate; hydrolytic; transglycosylation; Xanthomonas campestris; Bacillus megaterium.},
   abstract = {Some microbes can produce hydrolytic enzymes and have transglycosylation capacity at the same time, including Xanthomonas campestris and Bacillus megaterium. The enzyme characteristics of the microbes can be observed from their activity by using several types of substrates. This research aimed to characterize the hydrolytic and transglycosylated CGTase enzyme activity from Xanthomonas campestris and Bacillus megaterium on glucose, tapioca, and corn starch media at several concentrations. Stages of research included bacterial rejuvenation, growth on the substrate, and analysis (hydrolytic activity, carbohydrate concentration, and transglycosylation activity). The research design used was a Completely Randomised Design (CRD). The data from observations were then analyzed using Analysis of Variance (ANOVA) with the Duncan Multiple Range Test (DMRT) at a 5% level to determine the effect of treatment on all observational variables. Data analysis was carried out using the SPSS Statistics 17.0 program. The analysis results showed Xanthomonas campestris and Bacillus megaterium with tapioca and cornstarch substrates of 2%, 4%, and 6%, respectively, had hydrolytic activity. CGTase enzymes produced from Xanthomonas campestris and Bacillus megaterium with glucose, tapioca, and corn starch substrates 2%, 4%, and 6%, had intramolecular transglycosylation activity. The CGTase hydrolytic activity test's analysis showed that the CGTase hydrolytic test's significance value was significantly different between treatments with an incubation time of 24 to 120 hours. The substrate, a carbon source, greatly influences the rate of enzyme production produced by microorganisms.},
   issn = {2088-5334},
   publisher = {INSIGHT - Indonesian Society for Knowledge and Human Development},
   url = {http://ijaseit.insightsociety.org/index.php?option=com_content&view=article&id=9&Itemid=1&article_id=12403},
   doi = {10.18517/ijaseit.11.1.12403}
}

EndNote

%A Herawati, Heny
%A Kamsiati, Elmi
%A Sunarmani, -
%A Munarso, S Joni
%A Supriyatna, Ina
%A Aminingsih, Tri
%D 2021
%T Hydrolytic and Transglycolation Characteristics of Xanthomonas campestris and Bacillus megaterium in Several Substrates
%B 2021
%9 Substrate; hydrolytic; transglycosylation; Xanthomonas campestris; Bacillus megaterium.
%! Hydrolytic and Transglycolation Characteristics of Xanthomonas campestris and Bacillus megaterium in Several Substrates
%K Substrate; hydrolytic; transglycosylation; Xanthomonas campestris; Bacillus megaterium.
%X Some microbes can produce hydrolytic enzymes and have transglycosylation capacity at the same time, including Xanthomonas campestris and Bacillus megaterium. The enzyme characteristics of the microbes can be observed from their activity by using several types of substrates. This research aimed to characterize the hydrolytic and transglycosylated CGTase enzyme activity from Xanthomonas campestris and Bacillus megaterium on glucose, tapioca, and corn starch media at several concentrations. Stages of research included bacterial rejuvenation, growth on the substrate, and analysis (hydrolytic activity, carbohydrate concentration, and transglycosylation activity). The research design used was a Completely Randomised Design (CRD). The data from observations were then analyzed using Analysis of Variance (ANOVA) with the Duncan Multiple Range Test (DMRT) at a 5% level to determine the effect of treatment on all observational variables. Data analysis was carried out using the SPSS Statistics 17.0 program. The analysis results showed Xanthomonas campestris and Bacillus megaterium with tapioca and cornstarch substrates of 2%, 4%, and 6%, respectively, had hydrolytic activity. CGTase enzymes produced from Xanthomonas campestris and Bacillus megaterium with glucose, tapioca, and corn starch substrates 2%, 4%, and 6%, had intramolecular transglycosylation activity. The CGTase hydrolytic activity test's analysis showed that the CGTase hydrolytic test's significance value was significantly different between treatments with an incubation time of 24 to 120 hours. The substrate, a carbon source, greatly influences the rate of enzyme production produced by microorganisms.
%U http://ijaseit.insightsociety.org/index.php?option=com_content&view=article&id=9&Itemid=1&article_id=12403
%R doi:10.18517/ijaseit.11.1.12403
%J International Journal on Advanced Science, Engineering and Information Technology
%V 11
%N 1
%@ 2088-5334

IEEE

Heny Herawati,Elmi Kamsiati,- Sunarmani,S Joni Munarso,Ina Supriyatna and Tri Aminingsih,"Hydrolytic and Transglycolation Characteristics of Xanthomonas campestris and Bacillus megaterium in Several Substrates," International Journal on Advanced Science, Engineering and Information Technology, vol. 11, no. 1, pp. 172-178, 2021. [Online]. Available: http://dx.doi.org/10.18517/ijaseit.11.1.12403.

RefMan/ProCite (RIS)

TY  - JOUR
AU  - Herawati, Heny
AU  - Kamsiati, Elmi
AU  - Sunarmani, -
AU  - Munarso, S Joni
AU  - Supriyatna, Ina
AU  - Aminingsih, Tri
PY  - 2021
TI  - Hydrolytic and Transglycolation Characteristics of Xanthomonas campestris and Bacillus megaterium in Several Substrates
JF  - International Journal on Advanced Science, Engineering and Information Technology; Vol. 11 (2021) No. 1
Y2  - 2021
SP  - 172
EP  - 178
SN  - 2088-5334
PB  - INSIGHT - Indonesian Society for Knowledge and Human Development
KW  - Substrate; hydrolytic; transglycosylation; Xanthomonas campestris; Bacillus megaterium.
N2  - Some microbes can produce hydrolytic enzymes and have transglycosylation capacity at the same time, including Xanthomonas campestris and Bacillus megaterium. The enzyme characteristics of the microbes can be observed from their activity by using several types of substrates. This research aimed to characterize the hydrolytic and transglycosylated CGTase enzyme activity from Xanthomonas campestris and Bacillus megaterium on glucose, tapioca, and corn starch media at several concentrations. Stages of research included bacterial rejuvenation, growth on the substrate, and analysis (hydrolytic activity, carbohydrate concentration, and transglycosylation activity). The research design used was a Completely Randomised Design (CRD). The data from observations were then analyzed using Analysis of Variance (ANOVA) with the Duncan Multiple Range Test (DMRT) at a 5% level to determine the effect of treatment on all observational variables. Data analysis was carried out using the SPSS Statistics 17.0 program. The analysis results showed Xanthomonas campestris and Bacillus megaterium with tapioca and cornstarch substrates of 2%, 4%, and 6%, respectively, had hydrolytic activity. CGTase enzymes produced from Xanthomonas campestris and Bacillus megaterium with glucose, tapioca, and corn starch substrates 2%, 4%, and 6%, had intramolecular transglycosylation activity. The CGTase hydrolytic activity test's analysis showed that the CGTase hydrolytic test's significance value was significantly different between treatments with an incubation time of 24 to 120 hours. The substrate, a carbon source, greatly influences the rate of enzyme production produced by microorganisms.
UR  - http://ijaseit.insightsociety.org/index.php?option=com_content&view=article&id=9&Itemid=1&article_id=12403
DO  - 10.18517/ijaseit.11.1.12403

RefWorks

RT Journal Article
ID 12403
A1 Herawati, Heny
A1 Kamsiati, Elmi
A1 Sunarmani, -
A1 Munarso, S Joni
A1 Supriyatna, Ina
A1 Aminingsih, Tri
T1 Hydrolytic and Transglycolation Characteristics of Xanthomonas campestris and Bacillus megaterium in Several Substrates
JF International Journal on Advanced Science, Engineering and Information Technology
VO 11
IS 1
YR 2021
SP 172
OP 178
SN 2088-5334
PB INSIGHT - Indonesian Society for Knowledge and Human Development
K1 Substrate; hydrolytic; transglycosylation; Xanthomonas campestris; Bacillus megaterium.
AB Some microbes can produce hydrolytic enzymes and have transglycosylation capacity at the same time, including Xanthomonas campestris and Bacillus megaterium. The enzyme characteristics of the microbes can be observed from their activity by using several types of substrates. This research aimed to characterize the hydrolytic and transglycosylated CGTase enzyme activity from Xanthomonas campestris and Bacillus megaterium on glucose, tapioca, and corn starch media at several concentrations. Stages of research included bacterial rejuvenation, growth on the substrate, and analysis (hydrolytic activity, carbohydrate concentration, and transglycosylation activity). The research design used was a Completely Randomised Design (CRD). The data from observations were then analyzed using Analysis of Variance (ANOVA) with the Duncan Multiple Range Test (DMRT) at a 5% level to determine the effect of treatment on all observational variables. Data analysis was carried out using the SPSS Statistics 17.0 program. The analysis results showed Xanthomonas campestris and Bacillus megaterium with tapioca and cornstarch substrates of 2%, 4%, and 6%, respectively, had hydrolytic activity. CGTase enzymes produced from Xanthomonas campestris and Bacillus megaterium with glucose, tapioca, and corn starch substrates 2%, 4%, and 6%, had intramolecular transglycosylation activity. The CGTase hydrolytic activity test's analysis showed that the CGTase hydrolytic test's significance value was significantly different between treatments with an incubation time of 24 to 120 hours. The substrate, a carbon source, greatly influences the rate of enzyme production produced by microorganisms.
LK http://ijaseit.insightsociety.org/index.php?option=com_content&view=article&id=9&Itemid=1&article_id=12403
DO  - 10.18517/ijaseit.11.1.12403