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Preliminary Study on Diverse Carbon Utilization by Transformant Aspergillus niger

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@article{IJASEIT204,
   author = {S. H. Mohammad and J. M. Jahim and Z. Nopiah and A. M. A. Murad and F. D. A. Bakar and R. Illias},
   title = {Preliminary Study on Diverse Carbon Utilization by Transformant Aspergillus niger},
   journal = {International Journal on Advanced Science, Engineering and Information Technology},
   volume = {2},
   number = {4},
   year = {2012},
   pages = {282--286},
   keywords = {A.niger; FF microplate; joining cluster analysis},
   abstract = {Aspergillus niger have been widely recognized as producer of metabolites and reported as good expression hosts for homologous and heterologous proteins. For recombinant expression systems, nature of metabolite production would change when the expression host system is modified via plasmid development. In order to study the diversity of carbon utilization of transformant A.niger and their relation to specific carbon sources that could trigger mannanase production, a new screening system was introduced using Biolog technique to evaluate the growth of the transformant performed on 95 carbon sources. As a result, the transformed A.niger were found able to utilize dextrin and other 27 carbohydrates with majority preferred carbohydrates were identified as monosaccharide, oligosaccharides and some sugar alcohols as the best chosen carbon sources for growth. The relative simplicity and global carbon sources underlying in the biolog system for screening of carbon source make it as a useful tool for the preliminary screening and identification of carbon sources in order to select the best carbon source for medium development.},
   issn = {2088-5334},
   publisher = {INSIGHT - Indonesian Society for Knowledge and Human Development},
   url = {http://ijaseit.insightsociety.org/index.php?option=com_content&view=article&id=9&Itemid=1&article_id=204},
   doi = {10.18517/ijaseit.2.4.204}
}

EndNote

%A Mohammad, S. H.
%A Jahim, J. M.
%A Nopiah, Z.
%A Murad, A. M. A.
%A Bakar, F. D. A.
%A Illias, R.
%D 2012
%T Preliminary Study on Diverse Carbon Utilization by Transformant Aspergillus niger
%B 2012
%9 A.niger; FF microplate; joining cluster analysis
%! Preliminary Study on Diverse Carbon Utilization by Transformant Aspergillus niger
%K A.niger; FF microplate; joining cluster analysis
%X Aspergillus niger have been widely recognized as producer of metabolites and reported as good expression hosts for homologous and heterologous proteins. For recombinant expression systems, nature of metabolite production would change when the expression host system is modified via plasmid development. In order to study the diversity of carbon utilization of transformant A.niger and their relation to specific carbon sources that could trigger mannanase production, a new screening system was introduced using Biolog technique to evaluate the growth of the transformant performed on 95 carbon sources. As a result, the transformed A.niger were found able to utilize dextrin and other 27 carbohydrates with majority preferred carbohydrates were identified as monosaccharide, oligosaccharides and some sugar alcohols as the best chosen carbon sources for growth. The relative simplicity and global carbon sources underlying in the biolog system for screening of carbon source make it as a useful tool for the preliminary screening and identification of carbon sources in order to select the best carbon source for medium development.
%U http://ijaseit.insightsociety.org/index.php?option=com_content&view=article&id=9&Itemid=1&article_id=204
%R doi:10.18517/ijaseit.2.4.204
%J International Journal on Advanced Science, Engineering and Information Technology
%V 2
%N 4
%@ 2088-5334

IEEE

S. H. Mohammad,J. M. Jahim,Z. Nopiah,A. M. A. Murad,F. D. A. Bakar and R. Illias,"Preliminary Study on Diverse Carbon Utilization by Transformant Aspergillus niger," International Journal on Advanced Science, Engineering and Information Technology, vol. 2, no. 4, pp. 282-286, 2012. [Online]. Available: http://dx.doi.org/10.18517/ijaseit.2.4.204.

RefMan/ProCite (RIS)

TY  - JOUR
AU  - Mohammad, S. H.
AU  - Jahim, J. M.
AU  - Nopiah, Z.
AU  - Murad, A. M. A.
AU  - Bakar, F. D. A.
AU  - Illias, R.
PY  - 2012
TI  - Preliminary Study on Diverse Carbon Utilization by Transformant Aspergillus niger
JF  - International Journal on Advanced Science, Engineering and Information Technology; Vol. 2 (2012) No. 4
Y2  - 2012
SP  - 282
EP  - 286
SN  - 2088-5334
PB  - INSIGHT - Indonesian Society for Knowledge and Human Development
KW  - A.niger; FF microplate; joining cluster analysis
N2  - Aspergillus niger have been widely recognized as producer of metabolites and reported as good expression hosts for homologous and heterologous proteins. For recombinant expression systems, nature of metabolite production would change when the expression host system is modified via plasmid development. In order to study the diversity of carbon utilization of transformant A.niger and their relation to specific carbon sources that could trigger mannanase production, a new screening system was introduced using Biolog technique to evaluate the growth of the transformant performed on 95 carbon sources. As a result, the transformed A.niger were found able to utilize dextrin and other 27 carbohydrates with majority preferred carbohydrates were identified as monosaccharide, oligosaccharides and some sugar alcohols as the best chosen carbon sources for growth. The relative simplicity and global carbon sources underlying in the biolog system for screening of carbon source make it as a useful tool for the preliminary screening and identification of carbon sources in order to select the best carbon source for medium development.
UR  - http://ijaseit.insightsociety.org/index.php?option=com_content&view=article&id=9&Itemid=1&article_id=204
DO  - 10.18517/ijaseit.2.4.204

RefWorks

RT Journal Article
ID 204
A1 Mohammad, S. H.
A1 Jahim, J. M.
A1 Nopiah, Z.
A1 Murad, A. M. A.
A1 Bakar, F. D. A.
A1 Illias, R.
T1 Preliminary Study on Diverse Carbon Utilization by Transformant Aspergillus niger
JF International Journal on Advanced Science, Engineering and Information Technology
VO 2
IS 4
YR 2012
SP 282
OP 286
SN 2088-5334
PB INSIGHT - Indonesian Society for Knowledge and Human Development
K1 A.niger; FF microplate; joining cluster analysis
AB Aspergillus niger have been widely recognized as producer of metabolites and reported as good expression hosts for homologous and heterologous proteins. For recombinant expression systems, nature of metabolite production would change when the expression host system is modified via plasmid development. In order to study the diversity of carbon utilization of transformant A.niger and their relation to specific carbon sources that could trigger mannanase production, a new screening system was introduced using Biolog technique to evaluate the growth of the transformant performed on 95 carbon sources. As a result, the transformed A.niger were found able to utilize dextrin and other 27 carbohydrates with majority preferred carbohydrates were identified as monosaccharide, oligosaccharides and some sugar alcohols as the best chosen carbon sources for growth. The relative simplicity and global carbon sources underlying in the biolog system for screening of carbon source make it as a useful tool for the preliminary screening and identification of carbon sources in order to select the best carbon source for medium development.
LK http://ijaseit.insightsociety.org/index.php?option=com_content&view=article&id=9&Itemid=1&article_id=204
DO  - 10.18517/ijaseit.2.4.204