Evaluating Pulses and Modified Fed-batch Feeding of Methanol to Increase Expression Level of Human Insulin Precursor in Pichia Pastoris High-Density Cultivation
How to cite (IJASEIT) :
P. Saeedi et al., “Global and regional diabetes prevalence estimates for 2019 and projections for 2030 and 2045: Results from the International Diabetes Federation Diabetes Atlas, 9th edition,” Diabetes Res. Clin. Pract., vol. 157, p. 107843, Nov. 2019, doi: 10.1016/j.diabres.2019.107843.
C. Bommer et al., “Global Economic Burden of Diabetes in Adults: Projections From 2015 to 2030.,” Diabetes Care, vol. 41, no. 5, pp. 963-970, May 2018, doi: 10.2337/dc17-1962.
M. C. Lawrence, “Understanding insulin and its receptor from their three-dimensional structure,” Mol. Metab., p. 101255, May 2021, doi: 10.1016/j.molmet.2021.101255.
C. Gurramkonda et al., “Application of simple fed-batch technique to high-level secretory production of insulin precursor using Pichia pastoris with subsequent purification and conversion to human insulin.,” Microb. Cell Fact., vol. 9, no. 31, pp. 1-11, May 2010, doi: 10.1186/1475-2859-9-31.
M. ZieliÅ„ski et al., “Expression and purification of recombinant human insulin from E. coli 20 strain,” Protein Expr. Purif., vol. 157, pp. 63-69, 2019, doi: https://doi.org/10.1016/j.pep.2019.02.002.
E. J. Thak, S. J. Yoo, H. Y. Moon, and H. A. Kang, “Yeast synthetic biology for designed cell factories producing secretory recombinant proteins,” FEMS Yeast Res., vol. 20, no. 2, Feb. 2020, doi: 10.1093/femsyr/foaa009.
J. Wu, G. Gong, S. Han, W. Zhang, Y. Hu, and L. Xie, “Expression, purification, and characterization of the Degludec precursor DesB30,” Protein Expr. Purif., vol. 161, pp. 28-39, 2019, doi: https://doi.org/10.1016/j.pep.2019.04.010.
R. J. Zahrl, D. A. Peña, D. Mattanovich, and B. Gasser, “Systems biotechnology for protein production in Pichia pastoris,” FEMS Yeast Res., vol. 17, no. 7, p. 68, Nov. 2017, doi: 10.1093/femsyr/fox068.
A.-M. Tran et al., “Pichia pastoris versus Saccharomyces cerevisiae: a case study on the recombinant production of human granulocyte-macrophage colony-stimulating factor,” BMC Res. Notes, vol. 10, no. 1, p. 148, Apr. 2017, doi: 10.1186/s13104-017-2471-6.
K. MarkoÅ¡oví¡, L. Weigneroví¡, M. Rosenberg, V. KÅ™en, and M. RebroÅ¡, “Upscale of recombinant α-L-rhamnosidase production by Pichia pastoris Mut(S) strain,” Front. Microbiol., vol. 6, p. 1140, Oct. 2015, doi: 10.3389/fmicb.2015.01140.
V. Looser, D. Lí¼thy, M. Straumann, K. Hecht, K. Melzoch, and K. Kovar, “Effects of glycerol supply and specific growth rate on methanol-free production of CALB by P. pastoris: functional characterisation of a novel promoter,” Appl. Microbiol. Biotechnol., vol. 101, no. 8, pp. 3163-3176, Apr. 2017, doi: 10.1007/s00253-017-8123-x.
X. Shi, T. Karkut, M. Chamankhah, M. Alting-Mees, S. M. Hemmingsen, and D. Hegedus, “Optimal conditions for the expression of a single-chain antibody (scFv) gene in Pichia pastoris,” Protein Expr. Purif., vol. 28, no. 2, pp. 321-330, Apr. 2003, doi: 10.1016/S1046-5928(02)00706-4.
A. A. Prabhu, V. D. Veeranki, and S. J. Dsilva, “Improving the production of human interferon gamma (hIFN-γ) in Pichia pastoris cell factory: An approach of cell level,” Process Biochem., vol. 51, no. 6, pp. 709-718, 2016, doi: https://doi.org/10.1016/j.procbio.2016.02.007.
M. Anggiani, I. Helianti, and A. Abinawanto, “Optimization of methanol induction for expression of synthetic gene Thermomyces lanuginosus lipase in Pichia pastoris,” in AIP Conference Proceedings, Oct. 2018, vol. 2023, no. 1, p. 20170, doi: 10.1063/1.5064154.
L. Jia, Z. Shi, J. Yan, J. Ding, and Q. Hua, “Enhancing heterologous proteins production by MutS Pichia pastoris via periodic methanol induction control,” AIChE J., vol. 66, no. 1, p. e16798, Jan. 2020, doi: 10.1002/aic.16798.
C. Dietzsch, O. Spadiut, and C. Herwig, “A fast approach to determine a fed batch feeding profile for recombinant Pichia pastoris strains,” Microb. Cell Fact., vol. 10, Oct. 2011, doi: 10.1186/1475-2859-10-85.
S. Katla, N. Mohan, S. S. Pavan, U. Pal, and S. Sivaprakasam, “Control of specific growth rate for the enhanced production of human interferon α2b in glycoengineered Pichia pastoris: process analytical technology guided approach,” J. Chem. Technol. Biotechnol., vol. 94, no. 10, pp. 3111-3123, Oct. 2019, doi: 10.1002/jctb.6118.
S. Polez et al., “A Simplified and Efficient Process for Insulin Production in Pichia pastoris.,” PLoS One, vol. 11, no. 12, p. e0167207, 2016, doi: 10.1371/journal.pone.0167207.
W.-C. Liu, S. Inwood, T. Gong, A. Sharma, L.-Y. Yu, and P. Zhu, “Fed-batch high-cell-density fermentation strategies for Pichia pastoris growth and production.,” Crit. Rev. Biotechnol., vol. 39, no. 2, pp. 258-271, Mar. 2019, doi: 10.1080/07388551.2018.1554620.
J. Zhang, Y. Zhao, M. Li, and T. Liu, “Optimization of defined medium for recombinant Komagataella phaffii expressing cyclodextrin glycosyltransferase,” Biotechnol. Prog., vol. 35, no. 5, p. e2867, Sep. 2019, doi: 10.1002/btpr.2867.
A. A. Prabhu, B. Mandal, and V. V. Dasu, “Medium optimization for high yield production of extracellular human interferon-γ from Pichia pastoris: A statistical optimization and neural network-based approach,” Korean J. Chem. Eng., vol. 34, no. 4, pp. 1109-1121, Apr. 2017, doi: 10.1007/s11814-016-0358-1.
D. Nurdiani, Hariyatun, N. Utami, E. Wahyu Putro, and W. Kusharyoto, “Selecting Pichia pastoris recombinant clones for higher secretion of human insulin precursor into the culture supernatant,” in IOP Conference Series: Earth and Environmental Science, Feb. 2020, vol. 439, no. 1, p. 12017, doi: 10.1088/1755-1315/439/1/012017.
D. Nurdiani, Hariyatun, and W. Kusharyoto, “Secretory expression of human insulin precursor in Pichia pastoris employing truncated alpha-factor leader sequence and a short C-peptide,” Indones. J. Biotechnol., vol. 23, no. 2, pp. 102-108, 2018, doi: 10.22146/ijbiotech.38958.
G. Gallo-Oller, R. Ordoñez, and J. Dotor, “A new background subtraction method for Western blot densitometry band quantification through image analysis software,” Journal of Immunological Methods, vol. 457. Elsevier B.V., pp. 1-5, Jun. 01, 2018, doi: 10.1016/j.jim.2018.03.004.
A. Tí¼rkanoÄŸlu í–zí§elik, S. Yılmaz, and M. Inan, “Pichia pastoris promoters,” in Methods in Molecular Biology, vol. 1923, Humana Press Inc., 2019, pp. 97-112.
X. Li et al., “Transcriptional analysis of impacts of glycerol transporter 1 on methanol and glycerol metabolism in Pichia pastoris,” FEMS Yeast Res., vol. 18, no. 1, p. 81, Feb. 2018, doi: 10.1093/femsyr/fox081.
M. Karbalaei, S. A. Rezaee, and H. Farsiani, “Pichia pastoris: A highly successful expression system for optimal synthesis of heterologous proteins,” Journal of Cellular Physiology, vol. 235, no. 9. Wiley-Liss Inc., pp. 5867-5881, Sep. 01, 2020, doi: 10.1002/jcp.29583.
C. B. Matthews, A. Kuo, K. R. Love, and J. C. Love, “Development of a general defined medium for Pichia pastoris,” Biotechnol. Bioeng., vol. 115, no. 1, pp. 103-113, Jan. 2018, doi: 10.1002/bit.26440.
A. Pekarsky, S. Mihalyi, M. Weiss, A. Limbeck, and O. Spadiut, “Depletion of boric acid and cobalt from cultivation media: Impact on recombinant protein production with Komagataella phaffii,” Bioengineering, vol. 7, no. 4, pp. 1-24, Dec. 2020, doi: 10.3390/bioengineering7040161.
W. Zhu, R. Xu, G. Gong, L. Xu, Y. Hu, and L. Xie, “Medium optimization for high yield production of human serum albumin in Pichia pastoris and its efficient purification,” Protein Expr. Purif., vol. 181, p. 105831, May 2021, doi: 10.1016/j.pep.2021.105831.
A. B. Zepeda, A. Pessoa, and J. G. Farías, “Carbon metabolism influenced for promoters and temperature used in the heterologous protein production using Pichia pastoris yeast,” Brazilian J. Microbiol., vol. 49, pp. 119-127, Nov. 2018, doi: 10.1016/j.bjm.2018.03.010.
X. Garcia-Ortega, N. Adelantado, P. Ferrer, J. L. Montesinos, and F. Valero, “A step forward to improve recombinant protein production in Pichia pastoris: From specific growth rate effect on protein secretion to carbon-starving conditions as advanced strategy,” Process Biochem., vol. 51, no. 6, pp. 681-691, 2016, doi: https://doi.org/10.1016/j.procbio.2016.02.018.
C. Canales, C. Altamirano, and J. Berrios, “The growth of Pichia pastoris Mut+ on methanol-glycerol mixtures fits to interactive dual-limited kinetics: model development and application to optimised fed-batch operation for heterologous protein production,” Bioprocess Biosyst. Eng., vol. 41, no. 12, pp. 1827-1838, Dec. 2018, doi: 10.1007/s00449-018-2005-1.
D. A. Peña, B. Gasser, J. Zanghellini, M. G. Steiger, and D. Mattanovich, “Metabolic engineering of Pichia pastoris,” Metabolic Engineering, vol. 50. Academic Press Inc., pp. 2-15, Nov. 01, 2018, doi: 10.1016/j.ymben.2018.04.017.
L. D. Picotto, G. H. Sguazza, M. A. Tizzano, C. M. Galosi, S. F. Cavalitto, and M. R. Pecoraro, “An effective and simplified DO-stat control strategy for production of rabies glycoprotein in Pichia pastoris,” Protein Expr. Purif., vol. 132, pp. 124-130, Apr. 2017, doi: 10.1016/j.pep.2017.02.004.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).