DOI : https://doi.org/10.18517/ijaseit.1.1.16

Characterization of Copolymer Dehypon® LS 54 and Its Application for Aqueous Two-Phase Systems Paired with the Waxy Maize Starch for Protein Extraction

Ario Betha Juanssilfero (1), Jamaliah Md. Jahim (2), Fariza Akmal Abdul Mutalib (3), Wan Zulaikha Wan Zulkifli (4), Farah Diba Abu Bakar (5), Osman Hassan (6)
(1) Chemical and Process Department, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia
(2) Chemical and Process Department, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia
(3) Chemical and Process Department, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia
(4) Chemical and Process Department, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia
(5) School of Bioscience and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia
(6) School of Bioscience and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia
Fulltext View | Download
How to cite (IJASEIT) :
Juanssilfero, Ario Betha, et al. “Characterization of Copolymer Dehypon® LS 54 and Its Application for Aqueous Two-Phase Systems Paired With the Waxy Maize Starch for Protein Extraction”. International Journal on Advanced Science, Engineering and Information Technology, vol. 1, no. 1, Feb. 2011, pp. 65-71, doi:10.18517/ijaseit.1.1.16.
Citation Format :
A thermo-separating aqueous two-phase system composed of Dehypon® LS 54, a polymeric surfactant and the waxy maize starch (amylopectin starch) has been used for partitioning of cutinase as a model protein. Dehypon® LS 54 were characterized by using 1H NMR spectroscopy to get information regarding the chemical structure and to confirm the presence of aliphatic moiety group in this copolymer. The phase diagram obtained for these novel polymer-polymers two-phase system shows two-phases with high polymer concentration. The waxy maize starch is enriched in the bottom phase while the copolymer Dehypon® LS 54 is found in the upper phase. Since this copolymer (Dehypon® LS 54) is thermo-reactive, the upper phase can be removed and heated above the copolymer’s cloud-point which resulting in the formation of a new two-phase system with a lower water phase, containing the target protein and an upper is copolymer-rich phase. Our results show that systems formed by waxy maize starch and Dehypon® LS 54 could become an alternative system to be used in large scale protein and enzyme purification due to their low cost, and also because they offer a viable solution to problems of polymer removal and recycling which makes this system more attractive.

Authors who publish with this journal agree to the following terms:

    1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
    2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
    3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).