DOI : https://doi.org/10.18517/ijaseit.13.5.18793

Phytochemical and Bioactive Compounds of Cocoa Beans as Supplement Ingredient Affected by Drying Methods

Fitri Setiyoningrum (1), Gunawan Priadi (2), Fifi Afiati (3), Lutfyah Chaerunissa (4), Maria Belgis (5), Urip Perwitasari (6), Ario Betha Juanssilfero (7), - Fahrurrozi (8), Elsera Br Tarigan (9), Dharma Vincentlau (10)
(1) Research Center for Applied Microbiology, National Research and Innovation Agency, Indonesia
(2) Research Center for Applied Microbiology, National Research and Innovation Agency, Indonesia
(3) Research Center for Applied Microbiology, National Research and Innovation Agency, Indonesia
(4) Department of Agricultural Product Technology, Faculty of Agricultural Technology, University of Jember, Indonesia
(5) Department of Agricultural Product Technology, Faculty of Agricultural Technology, University of Jember, Indonesia
(6) Research Center for Applied Microbiology, National Research and Innovation Agency, Indonesia
(7) Research Center for Applied Microbiology, National Research and Innovation Agency, Indonesia
(8) Research Center for Land and Marine Bio Industry, National Research and Innovation Agency, Indonesia
(9) Research Center for Pharmaceutical Ingredient and Traditional Medicine, National Research and Innovation Agency, Indonesia
(10) Research Center for Applied Microbiology, National Research and Innovation Agency, Indonesia
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How to cite (IJASEIT) :
Setiyoningrum, Fitri, et al. “Phytochemical and Bioactive Compounds of Cocoa Beans As Supplement Ingredient Affected by Drying Methods”. International Journal on Advanced Science, Engineering and Information Technology, vol. 13, no. 5, Oct. 2023, pp. 1756-62, doi:10.18517/ijaseit.13.5.18793.
Citation Format :
Cocoa beans are a common food ingredient recognized by the public because they can be made into various products and have health benefits such as antioxidant, anti-inflammatory, and anti-diabetic if appropriately processed. This study used the Forastero variety cultivated at Sukabumi region, Indonesia, as a cocoa bean sample. This study aimed to analyze phytochemical properties and the bioactive compounds content of cocoa beans using GC-MS affected by various drying methods. The methods used were sun drying, cabinet drying, and freeze drying. The observed parameters to determine the cacao bean quality were antioxidant capacity, phenol and flavonoid content, and bioactive compound. The result showed that the drying method affected dried cocoa beans' phenol and flavonoid content and antioxidant activity. However, the freeze-drying gave the best phytochemicals properties in phenol content (5,6785 mg GAE/g), flavonoid (64,33 mg QE/g), and antioxidant capacity (42,17% inhibition on DPPH 0,1mM). The result was in line with volatile compounds with proven high biological activity. Cabinet drying produced cocoa beans, which is better than sun drying. The freeze-drying maintains lactones, ketones, alcohols, aldehydes, and phenols. The freeze-dried cocoa beans had the highest content of γ-Tocopherol, 2,4-Di-tert-butylphenol, 5-hydroxymethylfurfural, neophytadiene, and palmitic acid as antioxidants. The freeze-drying method could be considered for producing dried cocoa beans as a supplement ingredient.

A. Winiarska-Mieczan and E. Tomaszewska, “Antioxidant, anti-inflammantory, and immunomodulatory properties of tea - The positive impact of tea consumption on patients with autoimmune diabetes,” Nutrients, vol. 13, pp. 1-25, 2021.

H. Khan et al., “Polyphenols in the treatment of autoimmune diseases,” Autoimmun. Rev., vol. 18, no. 7, pp. 647-657, 2019.

M. A. El-Missiry, A. Fekri, L. A. Kesar, and A. I. Othman, “Polyphenols are potential nutritional adjuvants for targeting COVID-19,” Phyther. Res., vol. 35, no. 6, pp. 2879-2889, 2021.

M. Maleki et al., “Stabilization of telomere by the antioxidant property of polyphenols: Anti-aging potential,” Life Sci., vol. 259, no. August, p. 118341, 2020.

D. Stagos, “Antioxidant activity of polyphenolic plant extracts,” Antioxidants, vol. 9, no. 1, 2020.

Y. Kawakami et al., “Effect of cacao polyphenol-rich chocolate on postprandial glycemia, insulin, and incretin secretion in healthy participants,” Nutrition, vol. 85, p. 111128, 2021.

E. Jean-Marie, D. Bereau, and J.-C. Robinson, “Benefits of polyphenols and methylxanthines from cocoa beans on dietary metabolic disorders,” Foods, vol. 10, no. 2049, pp. 1-20, 2021.

J. G. B. Borja Fajardo, H. B. H. Tellez, G. C. P. Atuesta, A. P. S. Aldana, and J. J. M. Arteaga, “Antioxidant activity, total polyphenol content and methylxantine ratio in four materials of Theobroma cacao L. from Tolima, Colombia,” Heliyon, vol. 8, no. 5, 2022.

J. G. B. Fajardo, H. B. H. Tellez, G. C. P. Atuesta, A. P. S. Aldana, and J. J. mendez Arteaga, “Antioxidant activity, total polyphenol content and methylxantine ratio in four materials of Theobroma cacao L . from Tolima, Colombia,” Heliyon, vol. 8, pp. 1-6, 2022.

E. Lembong, M. Djali, Zaida, and G. L. Utama, “The potential of dry fermented cocoa (Theobroma cacao L.) variety Lindak bean shell treated at different degrees of roasting as a functional food,” IOP Conf. Ser. Earth Environ. Sci., vol. 733, no. 1, 2021.

Fahrurrozi et al., “Effect of Small-Scale Box-Fermentation on Catechin and Epicatechin Content of Lampung Cocoa Beans Varieties,” Int. J. Adv. Sci. Eng. Inf. Technol., vol. 11, no. 3, pp. 1029-1034, 2021.

S. Baliyan et al., “Determination of Antioxidants by DPPH Radical Scavenging Activity and Quantitative Phytochemical Analysis of Ficus religiosa,” Molecules, vol. 27, no. 4, 2022.

E. Septianti, Salengke, and J. Langkong, “Profile of bioactive compounds, antioxidant and aromatic component from several clones of cocoa beans during fermentation,” IOP Conf. Ser. Earth Environ. Sci., vol. 575, no. 1, 2020.

L. Goya, J. E. Kongor, and S. de Pascual-Teresa, “From cocoa to chocolate: effect of processing on flavanols and methylxanthines and their mechanisms of action,” Int. J. Mol. Sci., vol. 23, no. 22, 2022.

N. Putriani, J. Perdana, Meiliana, and P. Y. Nugrahedi, “Effect of thermal processing on key phytochemical compounds in green feafy vegetables: A review,” Food Rev. Int., vol. 38, no. 4, pp. 783-811, 2022.

Y. Gao et al., “Impact of thermal processing on dietary flavonoids,” Curr. Opin. Food Sci., vol. 48, no. Figure 2, p. 100915, 2022.

A. S. Menon, C. L. Hii, C. L. Law, S. Shariff, and M. Djaeni, “Effects of drying on the production of polyphenol-rich cocoa beans,” Dry. Technol., vol. 35, no. 15, pp. 1799-1806, 2017.

H. Majid and H. A. Rining, “The Effect of Drying Techniques on the Antioxidant Capacity, Flavonoids and Phenolic Content of Fermented Local Cocoa,” J. Adv. Res. Appl. Mech., vol. 1, no. 1, pp. 11-19, 2018.

G. Strocchi et al., “Potential Aroma Chemical Fingerprint of Oxidised Coffee Note by HS-SPME-GC-MS and Machine Learning,” Foods, vol. 11, no. 24, pp. 1-14, 2022.

A. Choudhary, V. Kumar, S. Kumar, I. Majid, P. Aggarwal, and S. Suri, “5-Hydroxymethylfurfural (HMF) formation, occurrence and potential health concerns: recent developments,” Toxin Rev., vol. 40, no. 4, pp. 545-561, 2021.

J. N. W. Karyadi, I. Kamila, A. D. Saputro, and D. Ayuni, “The Effect of Blanching on the Quality of Freeze-Dried Edamame,” Int. J. Adv. Sci. Eng. Inf. Technol., vol. 12, no. 2, pp. 819-825, 2022.

L. Huang et al., “Simultaneous determination of α-tocopherol, β-tocopherol, γ-tocopherol, δ-tocopherol, sesamin, sesamolin, sesamol, and asarinin in sesame oil by normal-phase high performance liquid chromatography,” J. Food Compos. Anal., vol. 104, no. May, p. 104132, 2021.

F. Zhao, P. Wang, R. D. Lucardi, Z. Su, and S. Li, “Natural sources and bioactivities of 2,4-di-tert-butylphenol and its analogs,” Toxins (Basel)., vol. 12, no. 1, pp. 1-26, 2020.

A. O. Ojatula, “Screening and Evaluation of Bioactive Components of Methanol Root Extract of Anthocleista nobilis G . Don . by GCMS Analysis,” Res. Sq., pp. 1-25, 2020.

S. Zhu et al., “Palmitic acid inhibits prostate cancer cell proliferation and metastasis by suppressing the PI3K/Akt pathway,” Life Sci., vol. 286, no. 2021, pp. 1-9, 2021.

K. B. Dar et al., “Immunomodulatory efficacy of Cousinia thomsonii C.B. Clarke in ameliorating inflammatory cascade expressions,” J. Ethnopharmacol., vol. 300, no. 2023, pp. 1-12, 2023.

M. Bhardwaj, V. K. Sali, S. Mani, and H. R. Vasanthi, “Neophytadiene from Turbinaria ornata suppresses LPS-induced inflammatory response in RAW 264.7 macrophages and Sprague Dawley rats,” Inflammation, vol. 43, no. 3, pp. 937-950, 2020.

N. Shad, A. Javaid, and Q. Kanwal, “Antifungal and other bioactive constituents in roots of a halophytic weed Suaeda fruticosa,” Pak.J.Weed Sci. Res, vol. 28, no. 3, pp. 313-320, 2022.

A. S. M. Khalil, N. Giribabu, S. Yelumalai, H. Shahzad, E. K. Kilari, and N. Salleh, “Myristic acid defends against testicular oxidative stress, inflammation, apoptosis: Restoration of spermatogenesis, steroidogenesis in diabetic rats,” Life Sci., vol. 278, no. 2021, pp. 1-17, 2021.

Y. Zhou, F. Cao, F. Luo, and Q. Lin, “Octacosanol and health benefits: Biological functions and mechanisms of action,” Food Biosci., vol. 47, no. 2022, pp. 1-16, 2022.

S. Arora and G. Kumar, “Gas chromatography-mass spectrometry (GC-MS) determination of bioactive constituents from the methanolic and ethyl acetate extract of Cenchrus setigerus Vahl (Poaceae),” Pharma Innov., vol. 6, no. 11-I, pp. 635/1-635/6, 2017.

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