Exploring Antagonistic Candidate Fungi for Controling Pathogenic Fungi (Colletotricum gloeosporioides) Causing Anthracnose Disease in Kintamani Siam Orange Plants (Citrus Nobillis Lour Var. Hass)
How to cite (IJASEIT) :
Darsini, Ni Nyoman, et al. “Exploring Antagonistic Candidate Fungi for Controling Pathogenic Fungi (Colletotricum Gloeosporioides) Causing Anthracnose Disease in Kintamani Siam Orange Plants (Citrus Nobillis Lour Var. Hass)”. International Journal on Advanced Science, Engineering and Information Technology, vol. 7, no. 1, Feb. 2017, pp. 269-75, doi:10.18517/ijaseit.7.1.1919.
Citation Format :
Orange plantation center in Bali are located in Bangli Regency, Kintamani District. Kintamani orange plantations cultivated three types of oranges: tangerine, selayer, and mandarin oranges. The famous orange in Bali today is Kintamani orange, tangerine type. The typical flavor and aroma of Kintamani tangerine make it is favored by consumers from various regions. Based on the information from Bangli District Agriculture Office and the results of field surveys in the last two years (in 2013 and 2014), cultivation of orange in Kintamani has been infected with anthracnose disease. The disease is characterized by symptoms whereby brown twigs spread to the leaves and fruit, and the fruits which are about to be harvested rot simultaneously and eventually fall due to decay. This condition causes farmers to suffer significant losses. The cause of anthracnose on Kintamani orange is Colletotrichum gloeosporioides . The Control of these diseases has been carried out by farmers with various synthetic fungicides but the disease is still widespread. It is feared that the uncontrolled use of synthetic pesticides can harm the environment, cause resistance to C. gloeosporioides fungi, and kill non-target beneficial micro-organism. It is necessary to conduct research that aims to control anthracnose biologically to maintain the ecological balance and environmental safety. Based on the results of this research by exploring the fungi on healthy plants around orange trees infected with anthracnose diseases, nine isolates of antagonist candidate fungus were obtained based on colony color of fungal hyphae (IS1, IS2, IS3, IS4, IS5. IS6, IS7, IS8, IS9). Based on the test results of the in vitro dual culture, two candidates of antagonistic fungal isolates were selected, the isolates IS4 and IS7. It was because on day 7 after the second dual culture, these two isolates had the highest percentage of inhibition, 89.22% and 85.11 % respectively. Based on the conventional and molecular identifications, it is known that the two candidate isolates of antagonistic fungus (IS4 and IS7) belong to one species of the Aspergillus aculeatus.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).