DOI : https://doi.org/10.18517/ijaseit.8.6.7690

Analysis of Gelatin Adulteration in Edible Bird’s Nest using Fourier Transform Infrared (FTIR) Spectroscopy

Nurul H. Jamalludin (1), Nur A. Tukiran (2)
(1) Department of Biotechnology, Kulliyyah of Science, International Islamic University Malaysia (IIUM), IIUM Kuantan, 25200 Kuantan, Malaysia
(2) International Institute for Halal Research and Training (INHART), Level 3, KICT Building, International Islamic University Malaysia (IIUM), Jalan Gombak, 53100, Selangor, Malaysia
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How to cite (IJASEIT) :
Jamalludin, Nurul H., and Nur A. Tukiran. “Analysis of Gelatin Adulteration in Edible Bird’s Nest Using Fourier Transform Infrared (FTIR) Spectroscopy”. International Journal on Advanced Science, Engineering and Information Technology, vol. 8, no. 6, Dec. 2018, pp. 2355-9, doi:10.18517/ijaseit.8.6.7690.
Citation Format :
Fraudulent incorporation of cheaper materials such as porcine gelatin into edible bird’s nest (EBN) can evolve into a problem for reasons related to religious, allergy, ethical, and legal requirement. Thus, this study aimed to detect porcine gelatin in the processed EBNs by using a combination method of Fourier transform infrared (FTIR) and chemometrics analysis, Principal Component Analysis (PCA). The use of FTIR spectroscopy in food analysis is becomes more attractive because of its cost-effective nature, nondestructive measurements as well as convenience for screening purposes. This method has been established to be useful for adulteration detection and quantification in various food products. However, its application as a sole method is often not reliable as some transitions of the spectrums are very complex or weak, making evaluation difficult. Thus, in this study FTIR data were further analysed with the chemometrics analysis. By considering all the data obtained, chemometrics makes better results feasible. Porcine gelatin exhibited a dominant band at Amide I indicating the adulteration of EBNs with porcine gelatin. The FTIR spectra were analysed using PCA in order to identify the adulteration percentage in the samples. In the assessment of the spiked samples, this method could detect at the minimum of 5% of porcine gelatin in EBNs. This method would be advantageous for ensuring quality of the EBN products in the market.

J. R. Lin, Y. Dong, H. Zhou, and X. P. Lai, “Identification of Edible Bird’s Nest by Electrophoresis,” World Science and Technology/Modernization of Traditional Chinese Medicine 30-32, 2006.

Y. Wu, Y. Chen, B. Wang, L. Bai, R. Wu, Y. Ge, and F. Yuan, “Application of SYBRgreen PCR and 2DGE methods to authenticate edible bird's nest food,” Food Res Int. 2020-2026, 2010.

L. Guo, Y. Wu, M. Liu, B. Wang, Y. Ge, and Y. Chen, “Authentication of Edible Bird's nests by TaqMan-based real-time PCR,” Food Control 220-226, 2014.

A. M. Ismail, A. Aina, D. M. Hashim, and I. Amin, “Using amino acids composition combined with principle component analysis to differentiate house and cave bird’s nest,” Current Trends in Technology and Science 363-366, 2013.

T. Nur Azira, I. Amin, M. Shuhaimi, and H. Muhajir, “Enzyme immunoassay for the detection of porcine gelatine in edible bird’s nest,” Food Addit Contam Part A 1023-1028, 2015.

T. Nur Azira, I. Amin, M. Shuhaimi, and H. Muhajir, “Determination of porcine gelatin in edible bird’s nest by competitive indirect ELISA based on anti-peptide polyclonal antibody,” Food Control 561 - 566, 2016.

N. Cebi, M. T. Yilmaz, and O. Sagdic, “A rapid ATR-FTIR spectroscopic method for detection of sibutramine adulteration in tea and coffee based on hierarchical cluster and principal component analyses,” Food Chem 517-526, 2017.

C. S. Whei Miaw, C. Assis, A. R. C. Sales Silva, M. L. Cunha, M. M. Sena, and S. V. C. de Sauza, “Determination of main fruits in adulterated nectars by ATR-FTIR spectroscopy combined with multivariate calibration and variable selection methods,” Food Chem 272-280, 2018.

A. M. Jimenez-Carvelo, M. T. Osorio, A. Koidis, A. Gonzalez-Casado, and L. Cuadros-Rodriguez, “Chemometric classification and quantification of olive oil in blends with any edible vegetable oils using FTIR-ATR and Raman spectroscopy,” LWT - Food Sci Technol 174-184, 2017.

D. M. Hashim, Y. B. Che Man, R. Norakasha, M. Shuhaimi, Y. Salmah, and Z. A. Syahariza, “ Potential use of Fourier transform infrared spectroscopy for differentiation of bovine and porcine gelatins,” Food Chem 856 - 860, 2010.

C. Ma, M. Rout, and W. Y. Mock, “Study of oat globulin conformation by Fourier transform infrared spectroscopy,” J Agric Food Chem 3328 - 3344, 2001.

R. M. Silverstein, F. X. Webster, and D. J. Kiemle, Spectrometric Identification of Organic Compounds United States of America: John Wiley & Sons, Inc., 2005.

B. Avula, T. J. Smillie, Y. Wang, J. Zweigenbaum, and A. Khan, “Authentication of true cinnamon (Cinnamon verum) utilising direct analysis in real time (DART)-QToF-MS,” Food Addit Contam: Part A 1-8, 2015.

M. D. Luca, W. Terouzi, G. Ioele, F. Kzaiber, A. Oussama, F. Oliverio, R. Tauler, and G. Ragno, “Derivative FTIR spectroscopy for cluster analysis and classification of morocco olive oils,” Food Chem.,” 1113 - 1118, 2011.

M. Nemati, M. R. Oveisi, H, Abdollahi, and O. Sabzevari, “Differentiation of bovine and porcine gelatins using principal component analysis,” J. Pharm. Biomed. Anal., 485-492, 2004.

J. Gayo, and S. A. Hale, “Detection and quantification of species authenticity and adulteration in crabmeat using visible and near-infrared spectroscopy,” J Agric Food Chem., 585 - 592, 2007.

A. Chambery, G. Monaco, A. D. Maro, and A. Parente, “Peptide fingerprint of high quality Campania White wines by MALDI-TOF mass spectrometry,” Food Chem., 1283 - 1289, 2009.

D. S. Lee, S. B. Noh, S. Y. Bae, and K. Kim, “Characterization of fatty acids composition in vegetable oils by gas chromatography and chemometrics,” Anal Chim Acta., 163 - 175, 1998.

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