DOI : https://doi.org/10.18517/ijaseit.11.3.13045

Screening of Potential Tannase-producing Fungi from Local Agri-industrial By-products using a Plate Assay and Submerged Fermentation

Mohammad Syaril Ramli (1), Raseetha Siva (2), Nur Yuhasliza Abd Rashid (3), Shaiful Adzni Sharifudin (4), Noraini Samat (5), Sawarni Hasibuan (6), Mohd Nizam Lani (7), Azlina Mansor (8)
(1) Faculty of Applied Sciences, Universiti Teknologi MARA, 40450 Shah Alam Selangor, Malaysia
(2) Faculty of Applied Sciences, Universiti Teknologi MARA, 40450 Shah Alam Selangor, Malaysia
(3) Fermentation and Enzyme Technology Programme, Food Science and Technology Research Centre, MARDI Headquarter, 43400 Serdang, Selangor, Malaysia
(4) Fermentation and Enzyme Technology Programme, Food Science and Technology Research Centre, MARDI Headquarter, 43400 Serdang, Selangor, Malaysia
(5) Animal and Aquaculture Feed, Animal Sciences Research Centre, MARDI Headquarter,43400 Serdang, Selangor, Malaysia
(6) Industrial Engineering Department, Universitas Mercu Buana, JI Meruya Selatan No 1 Kembangan, West Jakarta 11650, Indonesia
(7) School of Food Science and Technology, Universiti Malaysia Terengganu, 21030 Kuala Terengganu, Terengganu, Malaysia
(8) Fermentation and Enzyme Technology Programme, Food Science and Technology Research Centre, MARDI Headquarter, 43400 Serdang, Selangor, Malaysia
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How to cite (IJASEIT) :
Ramli, Mohammad Syaril, et al. “Screening of Potential Tannase-Producing Fungi from Local Agri-Industrial By-Products Using a Plate Assay and Submerged Fermentation”. International Journal on Advanced Science, Engineering and Information Technology, vol. 11, no. 3, June 2021, pp. 1209-13, doi:10.18517/ijaseit.11.3.13045.
Citation Format :
Tannase (Tannin Acyl Hydrolase EC 3.1.1.20) is an industrial inducible enzyme capable of hydrolyzing hydrolyzable tannin ester linkage gallotannin and ellagitannin, producing gallic acid and glucose. Tannase is extensively used in the pharmaceutical, chemical, cosmetics, textile, leather, food, feed, and beverage industries. In the beverage industry, tannase is used as a clarifying agent to clarify tannin present in coffee, coffee-flavored soft drinks, tea, and fruit juices by removing phenolic compounds. In the pharmaceutical industry, tannase is used to produce gallic acid, an intermediary compound in the production of antibacterial drug, trimethoprim, while in the food industry, tannase is used to synthesize crucial antioxidant food preservative propyl gallate (3,4,5-trihydroxybenzoate). Most of the tannase production utilizes bacteria such as Bacillus sp. as tannase producer under submerged fermentation, SmF. Despite the immense industrial potential of tannase, it has not fully been exploited due to lack of knowledge, and fewer studies reported filamentous fungi for tannase production. This study aimed to screen potential tannase-producing fungi from various agri-industrial by-products such as rice by-products, spent tea, spent coffee ground, banana peels, mango peels, desiccated coconut residue, soybean residue, sweet potato peels, and onions. Fungal isolate, J1 (Aspergillus niger) was identified as the efficient tannase-producing fungus due to the hydrolytic zone's largest diameter (60.7 ± 0.6) mm. It achieved high tannase activity with (6.86 ± 0.04) U/ml in submerged fermentation, SmF. In conclusion, filamentous fungi isolated from agri-industrial by-products have huge potential as an efficient tannase producer.

M. R. Meini, L. L. Ricardi, and D. Romanini, “Novel Routes for Valorisation of Grape Pomace Through the Production of Bioactives by Aspergillus niger,” Waste and Biomass Valorization, vol. 11, no. 11, pp. 6047-6055, 2020, doi: 10.1007/s12649-019-00844-1.

F. Spennati et al., “The role of cosubstrate and mixing on fungal biofilm efficiency in the removal of tannins,” Environ. Technol. (United Kingdom), vol. 41, no. 26, pp. 3515-3523, 2020, doi: 10.1080/09593330.2019.1615128.

I. A. Aboagye and K. A. Beauchemin, “Potential of molecular weight and structure of tannins to reduce methane emissions from ruminants: A review,” Animals, vol. 9, no. 11, pp. 1-18, 2019, doi: 10.3390/ani9110856.

G. Maisetta, G. Batoni, P. Caboni, S. Esin, A. C. Rinaldi, and P. Zucca, “Tannin profile, antioxidant properties, and antimicrobial activity of extracts from two Mediterranean species of parasitic plant Cytinus,” BMC Complement. Altern. Med., vol. 19, no. 1, pp. 1-11, 2019, doi: 10.1186/s12906-019-2487-7.

P. Chaudhary, V. Chhokar, P. Choudhary, A. Kumar, and V. Beniwal, “Optimization of chromium and tannic acid bioremediation by Aspergillus niveus using Plackett-Burman design and response surface methodology,” AMB Express, vol. 7, no. 1, 2017, doi: 10.1186/s13568-017-0504-0.

T. Behl et al., “Exploring the multifocal role of phytochemicals as immunomodulators,” Biomed. Pharmacother., vol. 133, no. November 2020, p. 110959, 2021, doi: 10.1016/j.biopha.2020.110959.

D. P. Demarque, D. R. Callejon, G. G. de Oliveira, D. B. Silva, C. A. Carollo, and N. P. Lopes, “The role of tannins as antiulcer agents: a fluorescence-imaging based study,” Rev. Bras. Farmacogn., vol. 28, no. 4, pp. 425-432, 2018, doi: 10.1016/j.bjp.2018.03.011.

F. Bonelli, L. Turini, G. Sarri, A. Serra, A. Buccioni, and M. Mele, “Oral administration of chestnut tannins to reduce the duration of neonatal calf diarrhea,” BMC Vet. Res., vol. 14, no. 1, pp. 4-9, 2018, doi: 10.1186/s12917-018-1549-2.

N. M. Delimont, S. K. Rosenkranz, M. D. Haub, and B. L. Lindshield, “Salivary proline-rich protein may reduce tannin-iron chelation: A systematic narrative review,” Nutr. Metab., vol. 14, no. 1, pp. 1-16, 2017, doi: 10.1186/s12986-017-0197-z.

K. Murugan, S. Saravanababu, and M. Arunachalam, “Screening of tannin acyl hydrolase (E.C.3.1.1.20) producing tannery effluent fungal isolates using simple agar plate and SmF process,” Bioresour. Technol., vol. 98, no. 4, pp. 946-949, 2007, doi: 10.1016/j.biortech.2006.04.031.

K. Khorsandi, Z. Kianmehr, Z. Hosseinmardi, and R. Hosseinzadeh, “Anti-cancer effect of gallic acid in presence of low level laser irradiation: ROS production and induction of apoptosis and ferroptosis,” Cancer Cell Int., vol. 20, no. 1, pp. 1-14, 2020, doi: 10.1186/s12935-020-1100-y.

L. A. BenSaad, K. H. Kim, C. C. Quah, W. R. Kim, and M. Shahimi, “Anti-inflammatory potential of ellagic acid, gallic acid and punicalagin A&B isolated from Punica granatum,” BMC Complement. Altern. Med., vol. 17, no. 1, pp. 1-10, 2017, doi: 10.1186/s12906-017-1555-0.

N. M. Aborehab and N. Osama, “Effect of Gallic acid in potentiating chemotherapeutic effect of Paclitaxel in HeLa cervical cancer cells,” Cancer Cell Int., vol. 19, no. 1, pp. 1-13, 2019, doi: 10.1186/s12935-019-0868-0.

M. Kumar, A. Singh, V. Beniwal, and R. K. Salar, “Improved production of tannase by Klebsiella pneumoniae using Indian gooseberry leaves under submerged fermentation using Taguchi approach,” AMB Express, vol. 6, no. 1, p. 46, 2016, doi: 10.1186/s13568-016-0217-9.

S. Abdullah, R. C. Pradhan, M. Aflah, and S. Mishra, “Efficiency of tannase enzyme for degradation of tannin from cashew apple juice: Modeling and optimization of process using artificial neural network and response surface methodology,” J. Food Process Eng., vol. 43, no. 10, pp. 1-10, 2020, doi: 10.1111/jfpe.13499.

T. C. Cairns et al., “Functional exploration of co-expression networks identifies a nexus for modulating protein and citric acid titres in Aspergillus niger submerged culture,” Fungal Biol. Biotechnol., vol. 6, no. 1, pp. 1-18, 2019, doi: 10.1186/s40694-019-0081-x.

C. Wu, F. Zhang, L. Li, Z. Jiang, H. Ni, and A. Xiao, “Novel optimization strategy for tannase production through a modified solid-state fermentation system,” Biotechnol. Biofuels, vol. 11, no. 1, pp. 1-15, 2018, doi: 10.1186/s13068-018-1093-0.

A. Jana et al., “Biosynthesis, structural architecture and biotechnological potential of bacterial tannase: A molecular advancement,” Bioresour. Technol., vol. 157, pp. 327-340, 2014, doi: 10.1016/j.biortech.2014.02.017.

M. A. Manan and C. Webb, “Newly designed multi-stacked circular tray solid-state bioreactor: analysis of a distributed parameter gas balance during solid-state fermentation with influence of variable initial moisture content arrangements,” Bioresour. Bioprocess., vol. 7, no. 1, 2020, doi: 10.1186/s40643-020-00307-9.

M. M. A. Youssef, W. M. A. El-Nagdi, and D. E. M. Lotfy, “Evaluation of the fungal activity of Beauveria bassiana, Metarhizium anisopliae and Paecilomyces lilacinus as biocontrol agents against root-knot nematode, Meloidogyne incognita on cowpea,” Bull. Natl. Res. Cent., vol. 44, no. 1, p. 112, 2020, [Online]. Available: https://doi.org/10.1186/s42269-020-00367-z.

A. Kanpiengjai, C. Khanongnuch, S. Lumyong, D. Haltrich, T. H. Nguyen, and S. Kittibunchakul, “Co-production of gallic acid and a novel cell-associated tannase by a pigment-producing yeast, Sporidiobolus ruineniae A45.2,” Microb. Cell Fact., vol. 19, no. 1, pp. 1-12, 2020, doi: 10.1186/s12934-020-01353-w.

M. Mehta, M. Muddapur, and V. G. Shanmuga Priya, “Fungal Production of Tannase:A Review,” Int. J. Sci. Eng. Technol., vol. 755, no. 28, pp. 2277-1581, 2013.

E. Zakipour-Molkabadi, Z. Hamidi-Esfahani, M. A. Sahari, and M. H. Azizi, “A new native source of Tannase producer, Penicillium sp. EZ-ZH190: Characterization of the enzyme,” Iran. J. Biotechnol., vol. 11, no. 4, pp. 244-250, 2013, doi: 10.5812/ijb.11848.

R. Morganna, F. Cavalcanti, P. Henrique, D. O. Ornela, J. A. Jorge, and L. H. Souza, “Screening, Selection and Optimization of the Culture Conditions for Tannase Production by Endophytic Fungi Isolated from Caatinga,” J. Appl. Biol. Biotechnol., vol. 5, no. 01, pp. 1-9, 2017, doi: 10.7324/jabb.2017.50101.

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