DOI : https://doi.org/10.18517/ijaseit.11.3.13591

Carrier RNA (cRNA) Enhances dsDNA Recovery Extracted from Small Volume Spent Embryo Culture Medium

Ayu Mulia Sundari (1), Pritta Ameilia Iffanolida (2), Kresna Mutia (3), Naylah Muna (4), Eliza Mansyur (5), Andon Hestiantoro (6), Budi Wiweko (7), Muhammad Fauzi (8), Anom Bowolaksono (9)
(1) Cellular and Molecular Mechanisms in Biological System (CEMBIOS) Research Group, Department of Biology, Faculty of Mathematics and Natural Sciences of Universitas Indonesia, Depok 16424, West Java, Indonesia
(2) Human Reproduction, Infertility, and Family Planning Cluster, Indonesia Reproductive Medicine Research and Training Center, Faculty of Medicine Universitas Indonesia, Jakarta 10430, Indonesia
(3) Cellular and Molecular Mechanisms in Biological System (CEMBIOS) Research Group, Department of Biology, Faculty of Mathematics and Natural Sciences of Universitas Indonesia, Depok 16424, West Java, Indonesia Human Reproduction, Infertility, and Family Planning Cluster, Indonesia Reproductive Medicine Research and Training Center, Faculty of Medicine Universitas Indonesia, Jakarta 10430, Indonesia
(4) Human Reproduction, Infertility, and Family Planning Cluster, Indonesia Reproductive Medicine Research and Training Center, Faculty of Medicine Universitas Indonesia, Jakarta 10430, Indonesia
(5) Yasmin In Vitro Fertilization Clinic, Cipto Mangunkusumo General Hospital, Jakarta 10430, Indonesia
(6) Reproductive Immunoendocrinology Division, Department of Obstetrics and Gynecology, Faculty of Medicine Universitas Indonesia, Dr. Cipto Mangunkusumo Hospital, Jakarta 10430, Indonesia
(7) Reproductive Immunoendocrinology Division, Department of Obstetrics and Gynecology, Faculty of Medicine Universitas Indonesia, Dr. Cipto Mangunkusumo Hospital, Jakarta 10430, Indonesia
(8) Cellular and Molecular Mechanisms in Biological System (CEMBIOS) Research Group, Department of Biology, Faculty of Mathematics and Natural Sciences of Universitas Indonesia, Depok 16424, West Java, Indonesia Department of Diabetes, Endocrinology, and Nutrition, Graduates School of Medicine, Kyoto University, Yoshida-Konoe-cho, Sakyo-ku, Kyoto 606-8501, Japan
(9) Cellular and Molecular Mechanisms in Biological System (CEMBIOS) Research Group, Department of Biology, Faculty of Mathematics and Natural Sciences of Universitas Indonesia, Depok 16424, West Java, Indonesia
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How to cite (IJASEIT) :
Sundari, Ayu Mulia, et al. “Carrier RNA (cRNA) Enhances DsDNA Recovery Extracted from Small Volume Spent Embryo Culture Medium”. International Journal on Advanced Science, Engineering and Information Technology, vol. 11, no. 3, June 2021, pp. 1203-8, doi:10.18517/ijaseit.11.3.13591.
Citation Format :
Embryo spent culture medium has been intensively investigated, considering its promising feature for non-invasive bioanalytical techniques in in-vitro fertilization (IVF). Despite, isolating DNA from such samples is quite challenging due to its small volume. Carrier RNA is reported to exhibit DNA retrieval effects and commonly employed in various limited biological samples, but there are no reports regarding its benefit on embryo media. Therefore, we aim to evaluate the competence of cRNA on isolated DNA from embryo medium and analyzed its optimal volume as there are also no records respecting its ideal volume to obtain decent outcomes. Results showed that cRNA significantly increases DNA amounts in the cRNA treated group (p<0.001), but the D-4/D-5 medium yielded similar (p=0.684). Pearson test demonstrated no correlation between cRNA volume vs. total retrieved DNA (r=0.760, p=0.80), and Whole Genome Amplification (WGA) was shown to increase DNA in the treated group (p=0.022), but not in the untreated group (p=0.128). Additionally, electrophoresis successfully resulting in a thick and thin band of TH01 locus signifies the cRNA competence. In conclusion, our study suggests that cRNA addition is essential in embryo medium extraction as it increases initial DNA that crucial for downstream application. However, the optimal volume could not be determined in the current study since the initial amount of DNA in the medium is unknown. Obtained findings are expected to be a new input for subsequent research on DNA extraction.

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