Cross Flow Microfiltration System in Separating Fermented Nixtamal Corn for Preparation of Natural Folic Acid

Agustine Susilowati (1), Aspiyanto (2), Hakiki Melanie (3), Yati Maryati (4), Puspa D. Lotulung (5)
(1) Research Centre for Chemistry, National Research and Innovation Agency, KST BJ Habibie, Serpong, Banten, Indonesia
(2) Research Centre for Chemistry, National Research and Innovation Agency, KST BJ Habibie, Serpong, Banten, Indonesia
(3) Research Centre for Chemistry, National Research and Innovation Agency, KST BJ Habibie, Serpong, Banten, Indonesia
(4) Research Centre for Chemistry, National Research and Innovation Agency, KST BJ Habibie, Serpong, Banten, Indonesia
(5) Research Centre for Pharmaceutical Ingredients and Traditional Medicine, National Research and Innovation Agency, KST BJ Habibie, Serpong, South Tangerang, Banten, Indonesia
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Susilowati, Agustine, et al. “Cross Flow Microfiltration System in Separating Fermented Nixtamal Corn for Preparation of Natural Folic Acid”. International Journal on Advanced Science, Engineering and Information Technology, vol. 13, no. 6, Dec. 2023, pp. 2015-23, doi:10.18517/ijaseit.13.6.19248.
A purification process of biomass of nixtamal yellow corn fermented by Rhizopus oligosporus strain C1 (FNC-A) and Rhizopus sp (FNC-B) was performed using a microfiltration (MF) membrane with pore size 0.15 µm installed in a crossflow microfiltration (CFMF) system to obtain retentate and permeate fractions as natural folic acid preparations. CFMF process was conducted at room temperature with a pump motor frequency (PMF) of 10 Hz (flow rate of 3.5 L/min) and a transmembrane pressure (TMP) of 4 bar for 0, 15, 30, 45, 60, 75, and 90 minutes. Based on optimum folic acid, the results showed that the best treatments of FNC-B and FNC-B were achieved at 90 min, resulting in a folic acid increase in retentate of FNC-A and FNC- B of 72.61 and 95.26%, reducing sugar of 20.41 and 170.93% (1.7-folds), total sugars of 155.51% (1.55-folds) and 426.76% (4.27-folds), and dissolved protein of 55.33 and 39.20%, and decrease in total solids of 88.91 and 91.64%, respectively, compared to initial biomass of FNC-A and FNC-B. The MFCF system effectively separated folic acid in retentates of FNC-A (33.77%) and FNC-B (95.27%) at the optimal condition. Folic acid monomers predominated the characteristics of FNC-A and FNC-B in optimum conditions with molecular weights of 442.10 and 442.18 Dalton, the average particle size of 38.31 μm and 37.97 μm, and distribution of particles at 10, 50, and 90% from the particle size 10.31, 26.32 and 81.55 μm, and 10.37, 28.04 and 76.09 μm, respectively.

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