Metabolite Compounds of Euglena sp. on Mass Cultivation System under MgCl2 and CaCl2 Salt Stress

Eko Agus Suyono (1), Dwi Hardianti Luthfiana (2), Raihan (3), Dedy Kurnianto (4), Khusnul Qonita Maghfiroh (5), Ria Amelia (6), Tia Erfianti (7), Arief Budiman (8), Nugroho Dewayanto (9)
(1) Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Sekip Utara, Sleman, Yogyakarta, Indonesia
(2) Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Sekip Utara, Sleman, Yogyakarta, Indonesia
(3) Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Sekip Utara, Sleman, Yogyakarta, Indonesia
(4) Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Sekip Utara, Sleman, Yogyakarta, Indonesia
(5) Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Sekip Utara, Sleman, Yogyakarta, Indonesia
(6) Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Sekip Utara, Sleman, Yogyakarta, Indonesia
(7) Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Sekip Utara, Sleman, Yogyakarta, Indonesia
(8) Chemical Engineering Department, Universitas Gadjah Mada, Jl. Grafika No. 2, Sleman, Yogyakarta, Indonesia
(9) Chemical Engineering Department, Universitas Gadjah Mada, Jl. Grafika No. 2, Sleman, Yogyakarta, Indonesia
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Suyono, Eko Agus, et al. “Metabolite Compounds of Euglena Sp. On Mass Cultivation System under MgCl2 and CaCl2 Salt Stress”. International Journal on Advanced Science, Engineering and Information Technology, vol. 14, no. 3, June 2024, pp. 1057-63, doi:10.18517/ijaseit.14.3.19820.
Euglena sp. is rich in primary and secondary metabolite compounds. This cosmopolitan organism can survive extreme conditions such as salt stress because it can increase cell growth rate and metabolism. This mass cultivation research uses an open pond system that is very vulnerable to contamination, so variations in salinity of MgCl2 and CaCl2 are used as treatments because they minimize contamination. The research examines the correlation of salt types to cell growth rate, biomass, monosaccharide profile, paramylon, pigment, and lipid of Euglena sp. Research methods include preparation, medium making, mass cultivation, and measurement of test parameters. Data were analyzed through One-Way ANOVA followed by DMRT, with a 5% confidence level. Based on the research result, the highest value of biomass and paramylon content and productivity at CaCl2 treatment with consecutive values 0.013 ± 0.001, 0.002 ± 0.000, 9.556 ± 0.070, and 0.149 ± 0.019. Monosaccharides analysis produced in the form of sucrose, glucose, fructose, and arabinose amounted to < 10 ppm. CaCl2 treatment produced the highest sucrose of 6251 ppm. The highest chlorophyll-a and carotenoid pigments are located at CaCl2 treatment, respectively (1.698 ± 0.051)b and (0.500 ± 0.032)b. At the same time, the salt treatment has a significant effect on lipid content. To summarize, almost all metabolite compounds were highest in CaCl2 treatment. This research is expected to contribute to developing the Euglena sp. mass cultivation system as a source of bioenergy and biomaterials.

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