DOI : https://doi.org/10.18517/ijaseit.14.3.19865

Chemical Component Analysis of Marine Sponge Extract of Potentially Medicinal Based on Solvent Differences

Ismail Marzuki (1), Bambang Heri Isnawan (2), Muhammad Arnol (3), Rahmiyah (4), Suciati (5), Arfandi Amin (6), Laode Muhamad Hafizh (7), Ruzkiah Asaf (8), Admi Athirah (9), Deni Aulia (10)
(1) Department of Chemical Engineering, Fajar University, Makassar, South Sulawesi, Indonesia
(2) Department of Agrotechnology, Universitas Muhammadiyah Yogyakarta, DI Yogyakarta, Indonesia
(3) Research Center for Fishery National Research and Innovation Agency (BRIN), Cibinong, West Java, Indonesia
(4) Research Center for Fishery National Research and Innovation Agency (BRIN), Cibinong, West Java, Indonesia
(5) Research Center for Fishery National Research and Innovation Agency (BRIN), Cibinong, West Java, Indonesia
(6) Research Center for Fishery National Research and Innovation Agency (BRIN), Cibinong, West Java, Indonesia
(7) Research Center for Conservation of Marine and Inland Water Resources, National Research and Innovation Agency (BRIN), Cibinong, West Java, Indonesia
(8) Research Center for Conservation of Marine and Inland Water Resources, National Research and Innovation Agency (BRIN), Cibinong, West Java, Indonesia
(9) Research Center for Conservation of Marine and Inland Water Resources, National Research and Innovation Agency (BRIN), Cibinong, West Java, Indonesia
(10) Department of Fisheries Biology, Collage of Fisheries Science, Pukyong National University, Busan, Republic of Korea
Fulltext View | Download
How to cite (IJASEIT) :
Marzuki, Ismail, et al. “Chemical Component Analysis of Marine Sponge Extract of Potentially Medicinal Based on Solvent Differences”. International Journal on Advanced Science, Engineering and Information Technology, vol. 14, no. 3, June 2024, pp. 1064-73, doi:10.18517/ijaseit.14.3.19865.
Citation Format :
Tracing and characterizing marine natural materials with special medicinal abilities is important to obtain new materials to enrich the diversity of drugs to treat particular diseases. The identification of the chemical components of sponges is interesting, considering the uniqueness and several other abilities possessed by sponges that are thought to have medicinal potential. Searching for chemical components in sponge extracts is suspected to contain secondary metabolic substances with medicinal potential. The study aimed to identify the chemical components of sponge extract with potential medicinal value based on differences in solvents. The first extract on the sponge Clathria (Thalysias) reinwardti  Sp.1 (CTR) by maceration method using semi-polar solvents (methanol). The second extraction uses a separating funnel with two types of non-polar solvents (diethyl-ether and n-hexane). The chemical components of the three extracts were analyzed using a gas chromatography-mass spectrophotometer instrument. The identified chemical components of sponge extract generally belong to the class of free fatty acids and cholesterol. Investigation of identified compounds with medicinal value chemical components, for example, Cholestan-3.alpha.-ol, Ergosta-5, Hexadecanoic acid, 1-(2,6-Dichlorophenyl)-2-indolinone and Pyridine-3-carboxamide. Searching for chemical components with medicinal potential from sponge extracts is novelty research based on differences in solvents, a new method. Complementing the data from this research, it is necessary to carry out further and specific searches related to chemical components with medicinal properties, which can be carried out by chromatographic fractionation and testing the activity of the extract against bacteria. pathogens and parasitic fungi.

D. Varijakzhan, J-Y. Loh, W-S. Yap, K. Yusoff, R. Seboussi, S-H. E. Lim, K-S. Lai, and C-M. Chong. “Bioactive Compounds from Marine Sponges: Fundamentals and Applications”. Mar Drugs. Vol.19, Num.5, p.246. 2021. doi: 10.3390/md19050246

R. Carroll, B. R. Copp, R. A. Davis, R. A. Keyzers, and M. R. Prinsep. “Marine natural products”. Nat. Prod. Rep. Vol. 39, pp. 122–173. 2022. doi: 10.1039/D1NP00076D

A. Sapar, M. Millenia, A. B. Aritonang, R. Rudiansyah, and W. Rahmalia. “Characterization of Secondary Metabolites and Cytotoxic Assay of Haliclona sp. Sponge Against T47d Breast Cancer Cells. Al Kimiya: Jurnal Ilmu Kimia dan Terapan, Vol. 10, Num. 1, pp. 40-49. 2023, doi: 10.15575/ak.v10i1.20334

S. Uras, and B. Konuklugil. “Anticancer secondary metabolites from marine sponges”. Ege Journal of Fisheries and Aquatic Sciences, Vol. 38, pp. 101-106. 2021, doi: 10.12714/egejfas.38.1.12

M-J, Chu, M. Li, H. Ma, P-L. Licd, and G-Q. Li. “Secondary metabolites from marine sponges of the genus Agelas: a comprehensive update insight on structural diversity and bioactivity”. Royal Society of Chemistry Advances, Vol. 27. Num.13. pp. 7594 – 8273. 2022, doi: 10.1039/D1RA08765G

V. Bhardwaj. “Marine Sponges (Scypha): Potential Source for Secondary Metabolites”. COJ Biomed Sci Res. Vol. 2, Num. 1. p. 000530. 2022, doi: 10.31031/COJBSR.2022.02.000530

Y.-J. Lee, Y. Cho, and H. N. K. Tran. “Secondary Metabolites from the Marine Sponges of the Genus Petrosia: A Literature Review of 43 Years of Research”. Mar Drugs, Vol. 19, p. 122. 2021, doi: 10.3390/md19030122

C. Abraham, D. J. Gochfeld, K. Macartney, A. Mellor, M. P. Lesser, and M. Slattery. “Biochemical variability in sponges across the Caribbean basin”. Invertebrate Biology, Vol. 140, Num. 3, p.e12341. 2021, doi: 10.1111/ivb.12341.

El-Demerdash, A. G. Atanasov, O. K. Horbanczuk, M. A. Tammam, M.–A. Mogib, J. N. A. Hooper, N. Sekeroglu, A. –Al. Mourabit, and A. Kijjoa. “Chemical Diversity and Biological Activities of Marine Sponges of the Genus Suberea: A Systematic Review”. Mar Drugs. Vol. 17, p. 115. 2021, doi: 10.3390/md17020115

G. Galitz, Y. Nakao, P. J. Schupp, G. Wörheide, and D. Erpenbeck. “A Soft Spot for Chemistry–Current Taxonomic and Evolutionary Implications of Sponge Secondary Metabolite Distribution”. Mar. Drugs, Vol. 19, Num. 8, p. 448. 2021, doi: 10.3390/md19080448

A. Sapar, M. R. Kurniawan, G. Gusrizal, A. Shofiyani, A. B. Aritonang, and E. Setiawan. “Sponge Biology Characteristics and Steroids Secondary Metabolite of Haliclona sp. from Lemukutan Island, West Kalimantan, Indonesia”. Asian Journal of Chemistry, Vol. 34, pp. 2373-78. 2022, doi: 10.14233/ajchem.2022.23824

I. Marzuki, S. Gusty, R. Armus, A. Sapar, R. Asaf, A. Athirah, and J. Jaya. “Secondary Metabolite Analysis and Anti-Bacteria and Fungal Activities of Marine Sponge Methanol Extract Based on Coral Cover”. In The 6th Int. Conf. on Basic Sciences (ICBS), IAP Conf. Proc. Vol. 2360, p. 040007.2021, doi: 10.1063/5.0059500

M. Elissawy, E. S. Dehkordi, N. Mehdinezhad, M. L. Ashour, and P. M. Pour. “Cytotoxic alkaloids derived from marine sponges: A comprehensive review”. Biomolecules, Vol. 11, Num. 2, p. 258. 2021, doi: 10.3390/biom11020258.

E. R. Abdelaleem, M. N. Samy, S. Y. Desoukey, M. Liu, R. J. Quinn, and U. R. Abdelmohsen. “Marine natural products from sponges (Porifera) of the order Dictyoceratida (2013 - 2019); a promising source for drug discovery”. RSC Advances, Vol. 10, pp. 34959–34976. 2020, doi: 10.1039/D0RA04408C

I. Marzuki, R. Asaf, and A. Athirah. “Bioremediation Performance of Marine Sponge Symbiont Bacteria against Nickel and Mercury Heavy Metal Pollutants”. Intern. J on Adv Sci, Eng and Inf Tech (Ijaseit), Vol. 13(2), pp. 544-552, 2023, doi: 10.18517/ijaseit.13.2.17581

H. U. Devkar, N. L. Thakur, P. Kaur. “Marine-derived antimicrobial molecules from the sponges and their associated bacteria”. Canadian Journal of Microbiology, Vol. 69, pp. 1-16. 2023, doi: 10.1139/cjm-2022-0147

I. Marzuki, M. Kamaruddin, and R. Ahmad. “Identification of marine sponges-symbiotic bacteria and their application in degrading polycyclic aromatic hydrocarbons”. Biodiversitas, Vol. 22, pp. 1481–8. 2021, doi: 10.13057/biodiv/d220352

D. S. Knopman, H. Amieva, R. C. Petersen, G. Chételat, D. M. Holtzman, B. T. Hyman, R. A. Nixon, and D. T. Jones. “Alzheimer’s disease. Nature Reviews Disease Primers, Vol. 7, pp. 1–21.2021, doi:10.1038/s41572-021-00269-y

E. Shafeian, P. G. Mostafavi M. M. Farimani, M. Moradi, and M. Nazemi. “Extraction and investigation of biological activities of dioctyl phthalate and dibutyl phthalate from marine sponge Haliclona (Soestella) caerulea Larak Island, Persian Gulf”. Iranian Journal of Fisheries Sciences, Vol. 21, Num. 5, pp. 1141-1155. 2022, doi:10.22092/ijfs.2022.127710

K. A. Krishnan, and T. R. Keerthi. “Analyses of Methanol Extracts of Two Marine Sponges, Spongia officinalis var. ceylonensis and Sigmadocia carnosa from Southwest Coast of India for their Bioactivities. Int.J.Curr.Microbiol.App.Sci., Vol. 5, Num 2, pp. 722-734. 2020, doi: 10.20546/ijcmas.2016.502.081

S. Sahidin, C. W. Sabandar, W. Wahyuni, R. Hamsidi, S. A. Mardikasari, W. O. S. Zubaydah, B. Sadarun, W. O. S. Musnina, A. Darmawan, A. Sundowo. “Investigation of compounds and biological activity of selected Indonesian marine sponges”. Natural Products Journal, Vol. 10. no 3, pp. 312-321. 2020, doi:10.2174/2210315509666190627105237

L. L. Hong, Y. F. Ding, W. Zhang, et al. “Chemical and biological diversity of new natural products from marine sponges: a review (2009–2018)”. Marine Life Sci Technol, Vol. 4, pp. 356–372. 2022, doi: 10.1007/s42995-022-00132-3

I. Marzuki, K. Nisaa, R. Asaf, R. Armus, M. Kamaruddin, A. Sapar, A. Emelda. “Biodegradation mechanism of naphthalene using marine sponge symbiotic bacteria”. 2nd International Conference on Fisheries and Marine 15 July 2021, Khairun University, Ternate, Indonesia (Virtual), IOP Conf. Ser. Earth Environ. Sci. Vol. 890, p. 012020. 2021, doi: 10.1088/1755-1315/890/1/012020

I. Marzuki, R. Asaf, M. Paena, A. Athirah, K. Nisaa, R. Ahmed, and K. Mudyawati. “Anthracene and Pyrene Biodegradation Performance of Marine Sponge Symbiont Bacteria Consortium”. Molecules, Vol. 26, p. 6851. 2021, doi: 10.3390/molecules26226851

I. Marzuki, R. Ahmad, M. Kamaruddin, R. Asaf, R. Armus, and I. Siswanty. “Performance of cultured marine sponges-symbiotic bacteria as a heavy metal bio-adsorption”. Biodiversitas, Vol. 22, pp. 5536-5543. 2021, doi: 10.13057/biodiv/d220352

I. Marzuki, E. Septiningsih, E. S. Kaseng, H. Herlinah, A. Sahrijanna, S. Sahabuddin, R. Asaf, A. Athirah, B. H. Isnawan, G. S. Samidjo, F. Rumagia, E. Hamidah, I. S. Santi, and K. Nisaa. “Investigation of Global Trends of Pollutants in Marine Ecosystems around Barrang Caddi Island, Spermonde Archipelago Cluster: An Ecological Approach”. Toxics. Vol. 10, p. 10060301. 2022, doi:10.3390/toxics10060301

P. Li, H. Lu, Y. Zhang, X. Zhang, L. Liu, M. Wang, and L. Liu. “The natural products discovered in marine sponge-associated microorganisms: structures, activities, and mining strategy”. Front. Mar. Sci., Vol. 19, 2023, doi: 10.3389/fmars.2023.1191858

K. Busch, E. Wurz, H. T. Rapp, K. Bayer, A. Franke, and U. Hentschel. “Chloroflexi Dominate the Deep-Sea Golf Ball Sponges Craniella zetlandica and Craniella infrequens Throughout Different Life Stages. Front. Mar. Sci. Vol. 7, pp. 1–12. 2020, doi:10.3389/fmars.2020.00674

M. Maslin, N. G. –Mazouni, C. Debitus, N. Joy, and R. Ho. “Marine sponge aquaculture towards drug development: An ongoing history of technical, ecological, chemical considerations and challenges”. Aquaculture Reports, Vol. 21, Nov. p. 100813. 2021, doi:10.1016/j.aqrep.2021.100813.

N. K. T. Dharmayani, I. Isnaini, M. Ulfa, S. Sudirman, E. Yuanita, B. N. Sariningsih. “Antibacterial Activity of Marine Sponge (Stylotella sp.)”. Journal of Research in Science Education, Vol. 9, Num.6, pp.4801–4805. 2023, doi: 10.29303/jppipa.v9i6.3839

S. F. Santosa, N. Nazaruddin, W. E. Sari, and F. Febriani. “Polyketide Synthase Gene Domain Exploration of Marine Sponge Symbiont Bacteria Collected from Weh Island”. Biosaintifika, Vol. 15, Num.2, pp. 246-254. 2023, doi: 10.15294/biosaintifika. v15i2.42980.

H. Hasan, R. Armus, M. Madania, and I. Marzuki. “Chemical Components of Artocarpus lanceifolius Roxb and Antioxidant Activity”. The 7th Inter. national Conference On Basic Sciences (ICBS), 23-24 Sept.2021 Ambon City, Indonesia, AIP Conf. Proceedings, Vol. 2588 p 020006. 2023, doi: 10.1063/5.0112313

D. P.-Puch, A. M. Forero, J. C. F. Monteverde, C. L.-Monterrubio, M. M.-Guitian, C. G-Salas, S. G. -Hernández, H. V. -Hernández, A. Beceiro, C. Griesinger, J. Rodríguez, and C. Jiménez. “Antimicrobial Diterpene Alkaloids from an Agelas citrina Sponge Collected in the Yucatán Peninsula”. Marine Drugs, Vol.20, p. 298. 2022, doi:10.3390/md20050298

H. Li, H. Yu, W. Wu, and P. Sun. “Chemical constituents of sponge Pseudoceratina sp. and their chemotaxonomic significance”. Biochemical Systematics and Ecology. Vol. 89, April, p. 104002. 2020, doi: 10.1016/j.bse.2020.104002

S. Depuydt, P. A. Patel, Z. Toplak, C. Bhat, V. M. Cová, I. Eteläinen, F. Vitulano, T. Bruun, A. Lempinen, N. Hribernik, E.-M. Lohiluoma, L. Hendrickx, E. L.-P. Junior, T. Tomašič, L. P. Mašič, J.-Y. Kauhaluoma, P. Kiuru, Tytgat, and S. Peigneur. “Structure-Function Studies of Sponge-Derived Compounds on the Cardiac Cav3.1 Channel”. Int. J. Mol. Sci. Vol. 24, p. 3429. 2023, doi: 10.3390/ijms24043429

Y. Y. Ho, N. F. Salleh, J. X. Goh, T. Emmanuel, O. Oluwabusola, and L. T. Tan. “Chemical nature of specialized defensive metabolites from seven common intertidal marine sponges found at Pulau Hantu, Singapore”. Nature In Singapore, Vol. 16, p. e2023108. 2023, doi:10.26107/NIS-2023-0108

Y. Retnowati, and A. S. Katili. “Antibacterial activity of sponge-associated bacteria from Torosiaje marine area, Gorontalo, Indonesia”. Biodiversitas, Vol. 24, pp. 1151-115. 2023, doi:10.13057/biodiv/d240255

M. Kamaruddin, I. Marzuki, A. Burhan, and R. Ahmad. “Screening acetylcholinesterase inhibitors from marine-derived actinomycetes by simple chromatography”, in The 1st International Conference on Biotechnology and Food Sciences 11 September 2020, Surabaya, Indonesia. IOP Conf. Series: Earth and Envi. Sci., Vol. 679. p. 012011. 2020, doi: 10.1088/1755-1315/679/1/012011

T. T. H. Dat, G. Steinert, N. T. K. Cuc, P. V. C. Smidt, and D. Sipkema. “Diversity of Bacterial Secondary Metabolite Biosynthetic Gene Clusters in Three Vietnamese Sponges”. Mar. Drugs, Vol. 21, p. 29. 2023, doi: 10.3390/md21010029

Y. S. Anteneh, Q. Yang, M. H. Brown, and C. M. M. Franco. “Antimicrobial activities of marine sponge-associated bacteria”. Microorganisms, Vol. 9, pp. 1–19. 2021, doi:10.3390/microorganisms9010171

L. Natoliy, D. Drozdov, A. Lyudmila, Z. Zemnukhova, E. Alexandr, P. Panasenko, V. Nataliya, P. Polyakova, B. Arseniy, S. Slobodyuk, Yu. Alexandr, U. Ustinov, A. Nina, D. Didenko, and A. T. Sergey. “Silicon Compounds in Sponges”. Appl. Science, Vol. 11, Num. 14, p. 6587. 2021, doi: 10.3390/app11146587

M. Mirsyah, I. Marzuki, and S. Gala. “Identifikasi Komponen Kimia Ekstrak Daun Katapangg (Terminalia catappa) Berdasarkan Perbandingan Metode Ekstraksi”. Al-Kimia, Vol. 10, pp. 70-83. 2022, doi: 10.24252/al-kimiav10i1.25457

N. A. Jamaludin, K. Bakar, and J. Saidin. “In Vitro Biological Activity of Three Marine Sponges From Theonella and Haliclona Genera Collected From Bidong Island, Terengganu, Malaysia”. Malaysian Applied Biology, Vol. 52, Num. 2, pp. 51-59. 2023, doi:10.55230/mabjournal.v52i2.2559

S. H. Babakr, M. E. Erez, M. Mukemre, and A. Dalar. “The phenolic profile and biological activities of common Scorzonera species from Eastern Anatolia”. Intern. Journal of Secondary Metabolite, Vol. 9, pp. 538–550, 2021, doi: 10.21448/ijsm.1084743

S. Sala, J. C. P. James, L. Gareth, L. Nealon, J. Fromont, O. Gomez, D. Vuong, E. Lacey, and G. R. Flematti. “Dendrillic Acids A and B: Nitrogenous, Rearranged Spongian Nor-Diterpenes from a Dendrilla sp. Marine Sponge”. Journal of Natural Products, Vol. 86, Num. 3, 482–489. 2023, doi: 10.1021/acs.jnatprod.2c01087

R. Armus, C. Selry, I. Marzuki, H. Hasan, S. Syamsia, and A. Sapar. “Investigation of Potential Marine Bacterial Isolates in Biodegradation Methods on Hydrocarbon Contamination”, in 2nd Workshop on Engineering, Education, Applied Sciences and Technology (WEAST), Makassar, Indonesia. Journal of Physics: Conference Series, Vol. 1899. p. 012006. 2022, doi: 10.1088/1742-6596/1899/1/012006

M. Maldonado, M. L.- Acosta, K. Busch, B. M. Slaby, K. Bayer, L. Beazley, U. Hentschel, E. Kenchingto, and H. T. Rapp. “A Microbial Nitrogen Engine Modulated by Bacteriosyncytia in Hexactinellid Sponges: Ecological Implications for Deep-Sea Communities”. Front. Mar. Sci. Vol. 8, pp. 1–35. 2021, doi: 10.3389/fmars.2021.638505

G. Atanasov, S. B. Zotchev, V. M. Dirsch,and C. T. Supuran. “Natural products in drug discovery: advances and opportunities”. Nat. Rev. Drug Discov. Vol. 20, Num. 3, pp. 200–216. 2021, doi:10.1038/s41573-020-00114-z

C.-W. Fu, L. Chiang, C.-H. Chao, Y.-L. Huang, S.-F. Chiou, L.-C. Wang, H.-W. Chang, S.-L. Chen, H.-C. Wang, M.-C. Yu, H.-C. Huang, and J.-H. Sheu. “Nakamusines A−C, new 9-methyladeninium diterpenoid alkaloids from a Formosan marine sponge Agelas nakamurai”. Tetrahedron, Vol. 149, p.133745. 2023, doi:10.1016/j.tet.2023.133745

Q.–He, S. Miao, N. –Ni, Y. Man, and K. Gong. “A Review of the Secondary Metabolites from the Marine Sponges of the Genus Aaptos”. Natural Product Communications, Vol. 15, pp. 1–12. 2020, doi: 10.1177/1934578X20951439

R. E. Abraham, M. Alghazwi, Q. Liang, and W. Zhang. “Advances on marine-derived natural radioprotection compounds: historic development and future perspective”. Mar Life Sci Technol, Vol. 3, pp. 474–487. 2021, doi: 10.1007/s42995-021-00095-x

S. Suciati, and L. Arifianti. “In vitro anticancer activity of marine sponges against T47D and Hela cell lines”. Dhaka Univ. J. Pharm. Sci., Vol. 19, Num. 1, pp. 25-28. 2020, www.banglajol.info/index.php/JPharma/artic.

S. A. Jokinen, T. Jilbert, R. T-. Filppula, and K. Koho. “Terrestrial organic matter input drives sedimentary trace metal sequestration in a human-impacted boreal estuary”. Sci. Total Environ. Vol. 717, p. 137047. 2020, doi: 10.1016/j.scitotenv.2020.137047

L. Pardosi, G. Fallo, and P. Jehaman. “Characterization and Identification of SM4 Bacterial Isolate from Stylissa Massa Sponse as Producing Antimicrobial Compounds Against Pathogenic Bacteria”. Jurnal Ilmu-Ilmu Hayati, Vol. 21, pp. 43–50. 2022, doi:10.14203/beritabiologi.v20i1.3991

T. J. Carrier, M. Maldonado, L. Schmittmann, L. Pita, T. C. G. Bosch, and U. Hentschel. “Symbiont transmission in marine sponges: reproduction, development, and metamorphosis”. BMC Biology, Vol. 20, pp. 1–19. 2022, doi: 10.1186/s12915-022-01291-6

I. Marzuki, I. Pratama, R. Asaf, A. Athirah, K. Nisaa, N. Nurbaya, M. Muslimin, N. Nurhidayah, A. Sahrijanna, and K. Kamaruddin. “Marine sponge symbiotic bacterial bioremediation against heavy metal pollutants in tiger prawns: Penaeus monodon culture medium”. Global J Envi Sci and Management, Vol. 10(3), pp. 1151-1170. 2024, doi: 10.22034/gjesm.2024.03.14

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with this journal agree to the following terms:

    1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
    2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
    3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).