DOI : https://doi.org/10.18517/ijaseit.15.1.19871

Morpho Genetic Variability and Anthocyanine (Cyanidin-3-O-Glucoside) Concent of Indonesia Roselle (Hibiscus sabdariffa L.)

Enung Sri Mulyaningsih (1), N.Sri Hartati (2), Wahyuni (3), Yuliana Galih Dyan Anggraheni (4), Rikno Harmoko (5), Hartati (6), Sri Indrayani (7), Nurhamidar Rahman (8), Hani Fitriani (9), Fiqolbi Nuro (10), Bustanussalam (11), Yatri Hapsari (12), Novelindo Rama Andika (13)
(1) RC for Genetic Engineering - National Research and Innovation Agency (BRIN), Indonesia
(2) RC for Genetic Engineering - National Research and Innovation Agency (BRIN), Indonesia
(3) RC for Genetic Engineering - National Research and Innovation Agency (BRIN), Indonesia
(4) RC for Genetic Engineering - National Research and Innovation Agency (BRIN), Indonesia
(5) RC for Genetic Engineering - National Research and Innovation Agency (BRIN), Indonesia
(6) RC for Genetic Engineering - National Research and Innovation Agency (BRIN), Indonesia
(7) RC for Applied Botany - National Research and Innovation Agency (BRIN), Indonesia
(8) RC for Applied Botany - National Research and Innovation Agency (BRIN), Indonesia
(9) RC for Applied Botany - National Research and Innovation Agency (BRIN), Indonesia
(10) RC for Applied Microbiology - National Research and Innovation Agency (BRIN), Indonesia
(11) RC for Vaccine and Drugs - National Research and Innovation Agency (BRIN), Indonesia
(12) RC for Vaccine and Drugs - National Research and Innovation Agency (BRIN), Indonesia
(13) Faculty of Agricultural Technology, Brawijaya University, Malang, Indonesia
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[1]
E. S. Mulyaningsih, “Morpho Genetic Variability and Anthocyanine (Cyanidin-3-O-Glucoside) Concent of Indonesia Roselle (Hibiscus sabdariffa L.)”, Int. J. Adv. Sci. Eng. Inf. Technol., vol. 15, no. 1, pp. 240–248, Feb. 2025.
Citation Format :
Roselle (Hibiscus sabdariffa L.) is a popular plant due to its colored calyces for herbal medicines and its stem for fibers. The development of new varieties of roselle with high-accumulating anthocyanins and productivity has become one of the breeding targets. Local varieties and selected accession roselle may be used as potential sources of important traits for breeding. Therefore, this study aimed to evaluate the phenotypes, genetics, and anthocyanin (cyanidin-3-O-glucoside) levels of four varieties and roselle accession with high yields that were grown at Cibinong, West Java, Indonesia. Results on phenotypic characterization in all genotypes showed variations in the red color intensities in flower organs. The color intensities of calyces and corollas are related; calyces with darker color intensities have darker color intensities in corollas. Genetic analysis using AFLP and RAPD markers revealed a high genetic diversity index, with average heterozygosity and diversity levels of 1 and 0.71 for AFLP and 0.995 and 0.63 for RAPD, respectively. The anthocyanin of cyanidin-3-O-glucoside levels ranged from 0.07 mg/100 g flower dry weight (FDW) in green roselle genotypes to 446 mg/100 g FDW in Roselindo 4. Two accession roselles, RUB1 and RUA7, showed the highest potential yields of dry calyx, reaching up to 600 kg/ha. Roselindo 4 has higher anthocyanins and RUB1 and RUA7 have higher potential yields. These are the parents we will use in the future for breeding new roselle cultivars that have higher anthocyanins and higher yields.

D. V. Deshmukh and R. S. Wagh, “Physiological analysis of calyx growth and yield analysis in roselle (Hibiscus sabdariffa L.),” Int. J. Bio-resource Stress Manag., vol. 15, no. 2, pp. 1–8, 2024, doi:10.23910/1.2024.5034.

G. K. Mahunu, H. E. Tahir, M. Osei-Kwarteng, A. A. Mariod, and J. P. Gweyi-Onyango, “Food use of whole and extracts of Hibiscus sabdariffa,” Roselle (Hibiscus sabdariffa), pp. 123–136, 2021, doi:10.1016/b978-0-12-822100-6.00009-4.

K. Akter et al., “Comparative morpho-agronomic and biochemical profiling of different roselle morphotypes based on their growth and yield associated attributes,” J. Agric. Crop., vol. 9, no. 94, pp. 514–523, 2023, doi: 10.32861/jac.94.214.523.

A. Zand-Silakhoor, H. Madani, H. H. Sharifabad, M. Mahmoudi, and G. Nourmohammadi, “Influence of different irrigation regimes and planting times on the quality and quantity of calyx, seed oil content, and water use efficiency of roselle (Hibiscus sabdariffa L.),” Grasas y Aceites, vol. 73, no. 3, 2022, doi: 10.3989/gya.0564211.

Y. Halim, C. Evelyne, D. Rosa, and S. Ramli, “Development of roselle (Hibiscus sabdariffa L.) calyx jelly candy,” Caraka Tani J. Sustain. Agric., vol. 37, no. 2, pp. 357–372, 2022, doi:10.20961/carakatani.v37i2.61537.

A. A. Lema, N. H. Mahmod, M. M. Khandaker, N. F. H. Nudin, and A. M. Dogara, “The effects of various drying techniques on physicochemical and nutritional qualities of roselle (Hibiscus sabdariffa L.) calyx,” Plant Sci. Today, vol. 9, no. 4, pp. 926–933, 2022, doi: 10.14719/pst.1796.

S. Saha, S. Ganguly, and D. Sikdar, “A review on anthocyanin pigments with respect to its nutraceutical properties,” Int. J. Mod. Trends Sci. Technol., vol. 6, no. 12, pp. 54–60, 2020, doi:10.46501/ijmtst061211.

M. S. Triyastuti and N. Anwar, “Bioactive compounds from purple roselle calyx (Hibiscus sabdariffa L.) extract using multistage countercurrent method,” Natl. Nutr. J., vol. 17, no. 1, pp. 1–10, 2022, doi: 10.20473/mgi.v17i1.1-10.

T. H. Kouakou, N. G. Konkon, K. Ayolié, A. P. Obouayeba, Z. H. Abeda, and M. Koné, “Anthocyanin production in calyx and callus of roselle (Hibiscus sabdariffa L.) and its impact on antioxidant activity,” J. Pharmacogn. Phytochem., vol. 4, no. 3, pp. 09–15, 2015.

S. Hartati, E. S. Mulyaningsih, H. Hartati, N. Rahman, Y. G. Dyan Anggraheni, and E. Sudarmonowati, “Karakterisasi fisiologi benih dan pertumbuhan tanaman rosela (Hibiscus sabdariffa L.) komersial,” J. Tumbuh. Obat Indones., vol. 14, no. 2, pp. 111–126, 2021, doi:10.22435/jtoi.v14i2.5182.

M. K. Serrano-Fuentes, F. C. Gómez-Merino, S. Cruz-Izquierdo, J. L. Spinoso-Castillo, and J. J. Bello-Bello, “Gamma radiation (60Co) induces mutation during in vitro multiplication of vanilla (Vanilla planifolia Jacks. ex Andrews),” Horticulturae, vol. 8, no. 6, pp. 1–12, 2022, doi: 10.3390/horticulturae8060503.

O. A. Y. Daudu, O. A. Falusi, M. C. Dangana, A. Abubakar, S. A. Yahaya, and D. R. Abejide, “Collection and evaluation of roselle (Hibiscus sabdariffa L.) germplasm in Nigeria,” Afr. J. Food Sci., vol. 9, no. 3, pp. 92–96, 2015, doi: 10.5897/ajfs2014.1225.

S. Konar, S. Adhikari, J. Karmakar, A. Ray, and T. K. Bandyopadhyay, “Evaluation of subculture ages on organogenic response from root callus and SPAR-based genetic fidelity assessment in the regenerants of Hibiscus sabdariffa L.,” Ind. Crops Prod., vol. 135, pp. 321–329, 2019, doi: 10.1016/j.indcrop.2019.04.018.

L. Braglia, S. Bruna, S. Lanteri, A. Mercuri, and E. Portis, “An AFLP-based assessment of the genetic diversity within Hibiscus rosa-sinensis and its place within the Hibiscus genus complex,” Sci. Hortic., vol. 123, no. 3, pp. 372–378, 2010, doi: 10.1016/j.scienta.2009.10.003.

M. Eldakak, S. Younes, H. Shalabi, H. Eltayeb, and M. Yacout, “Molecular marker analysis tools as a cornerstone for the phylogenetic analysis of Hibiscus species in Egypt,” New Val. J. Agric. Sci., vol. 1, no. 2, pp. 63–75, 2021, doi: 10.21608/nvjas.2021.99876.1012.

A. K. M. A. Omalsaad, M. A. Jahan, Z. Yaakob, and M. Osman, “Genetic relationship between roselle (Hibiscus sabdariffa L.) and kenaf (Hibiscus cannabinus L.) accessions through optimization of PCR-based RAPD method,” Emirates J. Food Agric., vol. 26, no. 3, pp. 247–258, 2014, doi: 10.9755/ejfa.v26i3.16498.

V. T. Nam et al., “Molecular markers for analysis of plant genetic diversity,” Vietnam J. Biotechnol., vol. 18, no. 4, pp. 589–608, 2021, doi: 10.15625/1811-4989/18/4/15326.

O. Daudu et al., “Assessment of genetic diversity among newly selected roselle (Hibiscus sabdariffa Linn.) genotypes in Nigeria using RAPD-PCR molecular analysis,” World J. Agric. Res., vol. 4, no. 3, pp. 64–69, 2016, doi: 10.12691/wjar-4-3-1.

M. F. Sari and A. Purwantoro, “Diversity analysis of 15 Hibiscus accession based on RAPD marker,” Ilmu Pertan. (Agricultural Sci.), vol. 3, no. 2, p. 89, 2019, doi: 10.22146/ipas.33211.

H. Hamidah and A. Z. Muhtadi, “Genetic Relationship of Hibiscus spp. Based on DNA bands Using RAPD Technique,” IOP Conf. Ser. Earth Environ. Sci., vol. 217, no. 1, p. 012024, 2019, doi: 10.1088/1755-1315/217/1/012024.

H. Mohd-Nasir, L. P. Wong, Z. A. Abdul Aziz, S. H. Mohd-Setapar, and H. Hassan, “The potential of roselle as health supplement: extraction, phytochemicals and future perspective,” IOP Conf. Ser. Mater. Sci. Eng., vol. 1051, no. 1, p. 012082, 2021, doi: 10.1088/1757-899X/1051/1/012082.

S. A. Nurfaradilla, F. C. Saputri, and Y. Harahap, “Effects of Hibiscus sabdariffa calyces aqueous extract on the antihypertensive potency of captopril in the two-kidney-one-clip rat hypertension model,” Evidence-Based Complement. Altern. Med., pp. 1–7, 2019, doi: 10.1155/2019/9694212.

N. Li, J. E. Simon, and Q. Wu, “Determination of anthocyanins, organic acids, and phenolic acids in hibiscus market products using LC/UV/MS,” J. Food Sci., vol. 89, no. 2, pp. 1098–1113, 2024, doi: 10.1111/1750-3841.16909.

D. Botstein, R. L. White, M. Skolnick, and R. W. Davis, “Construction of a genetic linkage map in man using restriction fragment length polymorphisms,” Am. J. Hum. Genet., vol. 32, no. 3, pp. 314–331, 1980.

G. A. Camelo-Méndez, J. A. Ragazzo-Sánchez, A. R. Jiménez-Aparicio, P. E. Vanegas-Espinoza, O. Paredes-López, and A. A. Del Villar-Martínez, “Comparative study of anthocyanin and volatile compounds content of four varieties of Mexican roselle (Hibiscus sabdariffa L.) by multivariable analysis,” Plant Foods Hum. Nutr., vol. 68, no. 3, pp. 229–234, 2013, doi: 10.1007/s11130-013-0360-2.

N. Hari Satyanarayana, V. Visalakshmi, S. Mukherjee, B. Priya, and K. K. Sarkar, “Genetic diversity in roselle (Hibiscus sabdariffa L.) for fibre yield,” Electron. J. Plant Breed., vol. 6, no. 3, pp. 826–830, 2015.

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