DOI : https://doi.org/10.18517/ijaseit.14.5.19988

Implementation of E.Dulcis as Albumin-coated Biomaterial Patch and Organic Photosensitizer for Photodynamic Therapy on Melanoma Cell Cancer

Totok Wianto (1), Eka Suarso (2), Ori Minarto (3), Sri Cahyo Wahyono (4), Retna Apsari (5), Amar Vijai Nasrulloh (6)
(1) Physics Study Program, University of Lambung Mangkurat, Jl. A. Yani Km.36 Banjarbaru, Indonesia
(2) Physics Study Program, University of Lambung Mangkurat, Jl. A. Yani Km.36 Banjarbaru, Indonesia
(3) Physics Study Program, University of Lambung Mangkurat, Jl. A. Yani Km.36 Banjarbaru, Indonesia
(4) Physics Study Program, University of Lambung Mangkurat, Jl. A. Yani Km.36 Banjarbaru, Indonesia
(5) Department of Engineering, Universitas Airlangga, Jl. Mulyorejo, Surabaya, Indonesia
(6) Physics Study Program, University of Lambung Mangkurat, Jl. A. Yani Km.36 Banjarbaru, Indonesia
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How to cite (IJASEIT) :
Wianto, Totok, et al. “Implementation of E.Dulcis As Albumin-Coated Biomaterial Patch and Organic Photosensitizer for Photodynamic Therapy on Melanoma Cell Cancer”. International Journal on Advanced Science, Engineering and Information Technology, vol. 14, no. 5, Oct. 2024, pp. 1756-62, doi:10.18517/ijaseit.14.5.19988.
Citation Format :
Photodynamic therapy (PDT) is a treatment modality that relies on photosensitizer administration and light-based activation. The use of chlorophyll as a photosensitizer is due to the abundance of chlorophyll in nature, especially in Indonesia, which is a tropical country. An obstacle to applying chlorophyll as a photosensitizer is the low stability of chlorophyll, so modifications are needed to obtain stable chlorophyll. The coating is a chlorophyll modification method that allows for a more straightforward synthesis and can increase its stability. This research aimed to implement the extracted Eleocharis dulcis (E.dulcis) as a chlorophyll source and modify it into an albumin-coated biomaterial as a photosensitizer. The modification results can be applied as patches to obtain albumin-modified biomaterial patches with maximum absorption at wavelengths between 650-750 nm and purity >95%. The photosensitizer chlorophyll coating results from the surface modification method of chlorophyll isolate of E.dulcis weed with albumin, which has stable, high hydrophilicity properties and low toxicity. The photosensitizer was then applied to achieve apoptotic melanoma cancer cell death, and red–near-infrared laser exposure was used to get high apoptotic results. In this research, PDT with a laser energy dose of 20 J/cm2, along with the addition of a photosensitizer on melanoma cell cancer, reached the highest percentage of apoptotic cell death for both MCF7 and T47D at 90 ± 0,8 % and 86 ± 0,7 % respectively. The potential of photodynamic therapy based on albumin-coated E.dulcis‘s chlorophyll as a biomaterial patch and organic photosensitizer in increasing the death of melanoma cell cancer is a significant outcome of this research.

A. F. Mironov et al., “Amino acid derivatives of natural chlorins as a platform for the creation of targeted photosensitizers in oncology,” Fine Chemical Technologies, vol. 15, no. 6, pp. 16–33, Jan. 2021, doi: 10.32362/2410-6593-2020-15-6-16-33.

S. A. Mosaddad et al., “Photodynamic Therapy in Oral Cancer: A Narrative Review,” Photobiomodulation, Photomedicine, and Laser Surgery, vol. 41, no. 6, pp. 248–264, Jun. 2023, doi:10.1089/photob.2023.0030.

A. Escudero, C. Carrillo-Carrión, M. C. Castillejos, E. Romero-Ben, C. Rosales-Barrios, and N. Khiar, “Photodynamic therapy: photosensitizers and nanostructures,” Materials Chemistry Frontiers, vol. 5, no. 10, pp. 3788–3812, 2021, doi: 10.1039/d0qm00922a.

E. Ostańska, D. Aebisher, and D. Bartusik-Aebisher, “The potential of photodynamic therapy in current breast cancer treatment methodologies,” Biomedicine & Pharmacotherapy, vol. 137, p. 111302, May 2021, doi: 10.1016/j.biopha.2021.111302.

J. H. Correia, J. A. Rodrigues, S. Pimenta, T. Dong, and Z. Yang, “Photodynamic Therapy Review: Principles, Photosensitizers, Applications, and Future Directions,” Pharmaceutics, vol. 13, no. 9, p. 1332, Aug. 2021, doi: 10.3390/pharmaceutics13091332.

Y. Ye, Y. Zhao, Y. Sun, and J. Cao, “Recent Progress of Metal-Organic Framework-Based Photodynamic Therapy for Cancer Treatment,” International Journal of Nanomedicine, vol. Volume 17, pp. 2367–2395, May 2022, doi: 10.2147/ijn.s362759.

T. Hu, Z. Wang, W. Shen, R. Liang, D. Yan, and M. Wei, “Recent advances in innovative strategies for enhanced cancer photodynamic therapy,” Theranostics, vol. 11, no. 7, pp. 3278–3300, 2021, doi:10.7150/thno.54227.

T. Kubrak, M. Karakuła, M. Czop, A. Kawczyk-Krupka, and D. Aebisher, “Advances in Management of Bladder Cancer—The Role of Photodynamic Therapy,” Molecules, vol. 27, no. 3, p. 731, Jan. 2022, doi: 10.3390/molecules27030731.

S. Lin, C. Liu, X. Han, H. Zhong, and C. Cheng, “Viral Nanoparticle System: An Effective Platform for Photodynamic Therapy,” International Journal of Molecular Sciences, vol. 22, no. 4, p. 1728, Feb. 2021, doi: 10.3390/ijms22041728.

I. S. Mfouo-Tynga, L. D. Dias, N. M. Inada, and C. Kurachi, “Features of third generation photosensitizers used in anticancer photodynamic therapy: Review,” Photodiagnosis and Photodynamic Therapy, vol. 34, p. 102091, Jun. 2021, doi:10.1016/j.pdpdt.2020.102091.

A. Al-Khafaji, M. Salman, H. Hassan, and A. Al-Shammari, “The Effect of NF-κB Deactivation on Cancer Cell Response to ALA Mediated Photodynamic Therapy,” Asian Pacific Journal of Cancer Prevention, vol. 25, no. 6, pp. 2051–2058, Jun. 2024, doi:10.31557/apjcp.2024.25.6.2051.

G. G. Eskiler et al., “The response of the canine mammary simple carcinoma and carcinosarcoma cells to 5-aminolaevulinic acid-based photodynamic therapy: An in vitro study,” Journal of Photochemistry and Photobiology B: Biology, vol. 234, p. 112509, Sep. 2022, doi:10.1016/j.jphotobiol.2022.112509.

K. A. D. F. Castro, J. A. Prandini, J. C. Biazzotto, J. P. C. Tomé, R. S. da Silva, and L. M. O. Lourenço, “The Surprisingly Positive Effect of Zinc-Phthalocyanines With High Photodynamic Therapy Efficacy of Melanoma Cancer,” Frontiers in Chemistry, vol. 10, Mar. 2022, doi: 10.3389/fchem.2022.825716.

T. E. Kim and J.-E. Chang, “Recent Studies in Photodynamic Therapy for Cancer Treatment: From Basic Research to Clinical Trials,” Pharmaceutics, vol. 15, no. 9, p. 2257, Aug. 2023, doi: 10.3390/pharmaceutics15092257.

H. Scheer, “Chlorophylls: A Personal Snapshot,” Molecules, vol. 27, no. 3, p. 1093, Feb. 2022, doi: 10.3390/molecules27031093.

J.-Q. Cai, X.-M. Liu, Z.-J. Gao, L.-L. Li, and H. Wang, “Chlorophylls derivatives: Photophysical properties, assemblies, nanostructures and biomedical applications,” Materials Today, vol. 45, pp. 77–92, May 2021, doi: 10.1016/j.mattod.2020.11.001.

C. Pucci, C. Martinelli, A. Degl’Innocenti, A. Desii, D. De Pasquale, and G. Ciofani, “Light‐Activated Biomedical Applications of Chlorophyll Derivatives,” Macromolecular Bioscience, vol. 21, no. 9, Jul. 2021, doi: 10.1002/mabi.202100181.

M. P. Uliana, A. da Cruz Rodrigues, B. A. Ono, S. Pratavieira, K. T. de Oliveira, and C. Kurachi, “Photodynamic Inactivation of Microorganisms Using Semisynthetic Chlorophyll a Derivatives as Photosensitizers,” Molecules, vol. 27, no. 18, p. 5769, Sep. 2022, doi: 10.3390/molecules27185769.

I. Ragusa, G. N. Nardone, S. Zanatta, W. Bertin, and E. Amadio, “Spirulina for Skin Care: A Bright Blue Future,” Cosmetics, vol. 8, no. 1, p. 7, Jan. 2021, doi: 10.3390/cosmetics8010007.

K. Chen et al., “Nature Products Chlorophyll Derivatives for NIR‐II Fluorescence Bioimaging and Plant‐Imaging,” Chemistry – A European Journal, Jul. 2024, doi: 10.1002/chem.202401805.

H. Eshghi, A. Sazgarnia, M. Rahimizadeh, N. Attaran, M. Bakavoli, and S. Soudmand, “Protoporphyrin IX–gold nanoparticle conjugates as an efficient photosensitizer in cervical cancer therapy,” Photodiagnosis and Photodynamic Therapy, vol. 10, no. 3, pp. 304–312, Sep. 2013, doi: 10.1016/j.pdpdt.2013.02.003.

X.-T. Yu, S.-Y. Sui, Y.-X. He, C.-H. Yu, and Q. Peng, “Nanomaterials-based photosensitizers and delivery systems for photodynamic cancer therapy,” Biomaterials Advances, vol. 135, p. 212725, Apr. 2022, doi: 10.1016/j.bioadv.2022.212725.

P. Theivendren et al., “A Recent Advancement in Nanotechnology Approaches for the Treatment of Cervical Cancer,” Anti-Cancer Agents in Medicinal Chemistry, vol. 23, no. 1, pp. 37–59, Jan. 2023, doi: 10.2174/1871520622666220513160706.

M. Chu, H. Li, Q. Wu, F. Wo, and D. Shi, “Pluronic-encapsulated natural chlorophyll nanocomposites for in vivo cancer imaging and photothermal/photodynamic therapies,” Biomaterials, vol. 35, no. 29, pp. 8357–8373, Sep. 2014, doi: 10.1016/j.biomaterials.2014.05.049.

Z.-Y. Zhang et al., “In vitro degradation, photo-dynamic and thermal antibacterial activities of Cu-bearing chlorophyllin-induced Ca–P coating on magnesium alloy AZ31,” Bioactive Materials, vol. 18, pp. 284–299, Dec. 2022, doi: 10.1016/j.bioactmat.2022.01.050.

E. Foresto, P. Gilardi, L. E. Ibarra, and I. S. Cogno, “Light-activated green drugs: How we can use them in photodynamic therapy and mass-produce them with biotechnological tools,” Phytomedicine Plus, vol. 1, no. 3, p. 100044, Aug. 2021, doi: 10.1016/j.phyplu.2021.100044.

E. Polat and K. Kang, “Natural Photosensitizers in Antimicrobial Photodynamic Therapy,” Biomedicines, vol. 9, no. 6, p. 584, May 2021, doi: 10.3390/biomedicines9060584.

L. Christina Pires Gonçalves, “Photophysical properties and therapeutic use of natural photosensitizers,” Journal of Photochemistry and Photobiology, vol. 7, p. 100052, Sep. 2021, doi: 10.1016/j.jpap.2021.100052.

T. Wianto, A. H. Zaidan, and R. Apsari, “Physical properties Characterization of Chlorophyll X Biomaterials Organic Exogenous Optical photosensitizer,” in Prosiding Seminar Natural and Material Seminar (NAMES 2015), 2015.

J. L. Araújo et al., “Photodynamic Therapy Directed to Melanoma Skin Cancer by Thermosensitive Hydrogel Containing Chlorophyll A,” Pharmaceuticals, vol. 16, no. 12, p. 1659, Nov. 2023, doi: 10.3390/ph16121659.

L. Liu, Y. Lu, Y. Fu, R. Horton, and T. Ren, “Estimating soil water suction from texture, bulk density and electrical resistivity,” Geoderma, vol. 409, p. 115630, Mar. 2022, doi:10.1016/j.geoderma.2021.115630.

C. Suwito and Y. Xi, “The effect of chloride-induced steel corrosion on service life of reinforced concrete structures,” Structure and Infrastructure Engineering, vol. 4, no. 3, pp. 177–192, Jun. 2008, doi: 10.1080/15732470600688699.

S. M. Milenković, J. B. Zvezdanović, T. D. Andelković, and D. Z. Marković, “The identification of chlorophyll and its derivatives in the pigment mixtures: HPLC-chromatography, visible and mass spectroscopy studies,” Adv. Technol., vol. 1, no. 1, pp. 16–24, 2012.

B. Voight and J. Sousa, “Lessons from Ontake-san: A comparative analysis of debris avalanche dynamics,” Engineering Geology, vol. 38, no. 3–4, pp. 261–297, Dec. 1994, doi: 10.1016/0013-7952(94)90042-6.

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