Identification of Essential Oil Components from Rose Flower with High Pulsed Electric Field (HPEF) Treatment using Water Distillation Method

Budi Hariono (1), Findi Citra Kusumasari (2)
(1) Department of Agricultural Technology, Politeknik Negeri Jember, Jember, Indonesia
(2) Department of Agricultural Technology, Politeknik Negeri Jember, Jember, Indonesia
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B. Hariono and F. C. Kusumasari, “Identification of Essential Oil Components from Rose Flower with High Pulsed Electric Field (HPEF) Treatment using Water Distillation Method”, Int. J. Adv. Sci. Eng. Inf. Technol., vol. 15, no. 1, pp. 164–172, Feb. 2025.
Rosa damascena Mill, frequently known as “damask rose” is a part of Rosaceae with an aroma in great demand by the public. Therefore, various derivative products utilize aromatic compounds from rose petals obtained through extraction, including essential oil. However, the effectiveness of extraction using the hydrodistillation method is still low. To address this issue, the modification of extraction process by combining HPEF as an initial pretreatment followed by hydrodistillation to enhance the quality and yield of rose essential oil was needed. The materials used were 5 kg of rose flowers and 40 liters of water as solvent. The method used was giving HPEF to the rose petals with an electric field of 7.5 kV/cm for 13 seconds and varying the frequency of HPEF (10 Hz, 20 Hz, 30 Hz). Furthermore, the rose petals that had passed the initial pretreatment went through the hydrodistillation stage by adding solvent. The distillate containing rose essential oil and solvent was separated based on the difference in density. The essential oil produced was quantified and analyzed for its compound using GC-MS. The results showed that HPEF treatment with a frequency of 20 Hz produced the highest essential oil yield (0.033%) and successfully extracted essential oils containing complex compounds such as 2-Hexyl-1-decanol and 1-nonadecene. The yield of essential oil produced still cannot be optimal, but there is an improvement compared to hydrodistillation without HPEF so further research can be carried out regarding the use of HPEF in the extraction process.

Direktorat Jenderal Penguatan Riset dan Pengembangan Kementerian Riset, Teknologi Dan Pendidikan Tinggi , "Prioritas Riset Nasional 2020-2024". 2020;1–93.

Kemenristekdikti, “Rencana Induk Riset Nasional Tahun 2017-2045”. Handb Logist Distrib. 2017;2045:47–8.

Ribkahwati, Purnobasuki. H, Isnaeni, Utami. E.S.W. Profil Minyak Atsiri Mahkota Bunga Mawar (Rosa Hybrida L.) Kultivar Lokal. Conference Paper : Universitas Airlangga. 2013.

Y. R. Suradinata and A. Wulansari, “Respon tanaman mawar batik (Rosa hybrida L.) dengan penggunaan konsentrasi 1–methylcyclopropene (1–MCP) pada beberapa tingkat kemekaran bunga,” Kultivasi, vol. 14, no. 2, pp. 55–62, 2015, doi: 10.24198/kultivasi.v14i2.12068.

D. Amiarsi and R. Tejasarwana, “Pengawet untuk Menjaga Kualitas Bunga Potong Mawar Selama Penyimpanan,” J. Hortik., vol. 21, no. 3, p. 274, 2016, doi: 10.21082/jhort.v21n3.2011.p274-279.

J. Genovese et al., “PEF-treated plant and animal tissues: Insights by approaching with different electroporation assessment methods,” Innov. Food Sci. Emerg. Technol., vol. 74, no. November, 2021, doi: 10.1016/j.ifset.2021.102872.

A. M. Hadri, Y. Benmimoun, K. Miloudi, Y. Bouhadda, S. T. Elsayed, and A. Hamimed, “Effect of Pulsed Electric Field Treatment on the Extraction of Essential Oil From Lavender (Lavandula angustifolia Mill.),” Int. J. Biol. Biotech, vol. 20, no. 1, pp. 37–46, 2023.

E. Fields, M. Barros, D. Rego, C. Serra, and K. Miloudi, “Extraction of Essential Oils from Plants by Hydrodistillation,” Appl. Sci., 2022.

M. Stoica, L. Mihalcea, D. Borda, and P. Alexe, “Non-thermal novel food processing technologies . An overview,” J. Agroaliment. Process. Technol., vol. 19, no. 2, pp. 212–217, 2013.

M. Carmelia Bălănică Dragomir, E. Daniela Zeca, A. Stela Ivan, and M. Stoica, “Pulsed electric field and high voltage electrical discharge-innovative food electrotechnologies. A review,” J. Agroaliment. Process. Technol., vol. 26, no. 1, pp. 34–39, 2020.

M. H. Pulungan, Sukardi, and N. Rizka, “Ekstraksi Minyak Atsiri Bunga Mawar dengan Metode Pelarut Menguap Menggunakan Perlakuan PEF (Pulsed Electric Field),” Indones. J. Essent. Oil, vol. 3, no. 1, pp. 26–36, 2018.

Y. Yuniati, S. N. Putri, P. Risang, R. Sambawa, D. S. Bhuana, and M. Mahfud, “Ekstraksi Minyak Atsiri dari Bunga Mawar (Rosa hybrda L.) dengan Metode Solvent-Free Microwave Extraction” Alchemy : journal of chemistry, vol 9, no. 2, 2021.

A. Damayanti and E. A, “Pemungutan Minyak Atsiri Mawar (Rose Oil) Dengan Metode Maserasi” J. Bahan Alam Terbarukan, vol. 4, no. 1, pp. 14–20, 2015, doi: 10.15294/jbat.v4i1.3769.

K. Miloudi, A. Hamimed, Y. Bouhadda, Y. Benmimoun, K. Belhouala, and B. Benarba, “Impact of pulsed electric field treatment for extracting essential oil from Mentha Spicata L,” Int. J. Electrochem. Sci., vol. 17, no. 8, p. 220829, 2022, doi: 10.20964/2022.08.41.

T. S. Julianto, Minyak Atsiri Bunga Indonesia. Yogjakarta. Deeppublish, 2014, pp. 119-120.

B. M. Lawrence, “Progress in essential oils,” Perfum. Flavorist, vol. 21, no. 2, pp. 25-28,30-32, 1996.

G. V. Barbosa-Cánovas, M.M. Góngora-Nieto, U. R. Pothakamury and B. G. Swanson. “Preservation of food with pulsed electric field”. Academic Press, United States of America. 1999.

Q. Zhang, G.V. Barbosa-Cfinovas, and B.G. Swanson, “Engineering aspects of pulsed electric field pasteurization”. J. Food Eng. 25, 261-281. 1995.

H. Vega-Mercado, O. Martin-Belloso, B. L. Qin, F. J. Chang, M. M. Gongora-Nieto, G. V. Barbosa-Cánovas and B. G. Swanson, “Non-thermal food preservation: pulsed electric fields”. Trends Food Sci Technol 8(5):151-157. 1996.

B. Hariono et al., “The design of a high-voltage pulsed electric field (hpef) device for non-liquid materials based on microcontroller,” IOP Conf. Ser. Earth Environ. Sci., vol. 1168, no. 1, 2023, doi: 10.1088/1755-1315/1168/1/012036.

A. Dobreva, F. Tintchev, V. Heinz, H. Schulz, S. Toepfl, “Effect of pulsed electric fields (PEF) on oil yield and quality during distillation of white oil-bearing rose (Rosa alba L.)”. Z Arznei- Gewurzpfla, 15(3): 127–135. 2010.

S. L. Harrison, G. V. Barbosa-Cánovas, B. G. Swanson, “”Saccharomyces cerevisiae structural changes induced by pulsed electric field treatment”. Lwt-Food Sci Technol, 30(3): 236–240. 1997.

S. Lin, Y. Guo, J. Liu, Q. You, Y. Yin, and S. Cheng, “Optimized enzymatic hydrolysis and pulsed electric field treatment for production of antioxidant peptides from egg white protein,” African J. Biotechnol., vol. 10, no. 55, pp. 11648–11657, 2011, doi: 10.5897/AJB11.1008.

I. Oktaviani, N. Letisya, P. Citra, D. P. Utari, I. N. A. Winata, W. Handayani, A. S. Nugraha, “Essential oil composition of rose flowers from Karangpring Village Jember district extracted by distillation and enfleurage”. Jurnal Ilmu Dasar, vol 20, no. 2, pp. 67-74. 2019.

C. Zhao, J. Xue, X. Cai, J. Guo, B. Li, S. Wu, “Assessment of the key aroma compounds in rose-based products”. Journal of Food and Drug Analysis, vol 24, pp. 471-476. 2016.

M. Mahboubi, N. Kazempour, T. Khamechian, M. H. Fallah, M. M. Kermani, “Chemical Composition and Antimicrobial Activity of Rosa damascena Mill Essential Oil”. Journal of Biologically Active Products from Nature, vol 1, no. 1, pp. 19-26. 2011.

H. Yuca, E. Tekman, A. Civa, S. Karakaya, G. Özturk, B. Demirci, A. Karaca, Z. Guvanelp, “Comparison of Essential Oils and Secretion Structures of Rosa damascena Mill. Grown in Iğdır and Isparta (Turkey)”. Sakarya University Journal of Science, vol 27, no. 3, pp. 523-529. 2023.

O. Üçüncü, C. Baltac, S. M. İlter, “Chemical composition, antimicrobial and antioxidant activities of essential oil from pedicularis condensata BIEB. Hittite Journal of Science and Engineering, vol 3, no. 2, pp.105-109. 2016.

R. Saeed, S. Gus, M. A. Khan, M. A. Kamboh, M. I. Khan, S. T. H. Sherazi, “GC-MS Evaluation of Essential Oil Constituents from Rosa Damascena Wild Rose: Effect of Season and Climatic Conditions,” Pak. J. Anal. Environ. Chem. vol 18, no. 2, pp. 155-162. 2017.

I. N. Utami, Y. Nurchayati, E. D. Hastuti, “Produksi dan Profil Metabolit Bunga Krisan (Chrysanthemum sp.) pada Intensitas Cahaya Lampu LED dengan Durasi Yang Berbeda”, Bioma, vol 21, no. 2, pp. 154-164. 2019.

I. Guterman, M. Shalit, N. Menda, D. Piestun, M. Dafny-Yelin, G. Shalev, D. Weiss, “Rose scent: genomics approach to discovering novel floral fragrance–related genes”, The Plant Cell, vol 14, no. 10, pp. 2325-2338. 2022.

A. Dobreva, D. Nedeltcheva-Antonova, N. Nenov, K. Getchovska, & L. Antonov, “Subcritical extracts from major species of oil-bearing roses—A comparative chemical profiling”, Molecules, vol 26, no. 16, pp. 4991. 2021.

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