International Journal on Advanced Science, Engineering and Information Technology, Vol. 4 (2014) No. 4, pages: 234-236, DOI:10.18517/ijaseit.4.4.407

Response Same Explant of Turi (Sesbania Grandiflora) in Shoot Induction Medium

Mardhiyetti Mardhiyetti, Zulfadly Syarif, Novirman Jamarun, Irfan Suliansyah

Abstract

Cotyledon, leaf, root and hipokotyl explants of Sesbania grandiflora were excised from germinated seedlings and cultured on Murashige & Skoog’s basal medium supplemented with various combinations of  cytokinin and auxin. Both cytokinin and auksin were required for induction of shoot. Medium of shoots induction has observed on MS medium supplement with BAP 1 mg/l and NAA 0.08 mg/l. On this medium, 66.7 % cultures responded with an average number of 1.7 shoots per explant cotiledon. However,  the average shoot length was limited to 0.7. whereas shoots induction obtained on MS medium with addition  1 mg/l BAP and 0.08 mg/l NAA average shoot length 0.5 cm. 50 % cultur responded with an average number of 1 shoots per explant.

Keywords:

Sesbania grandiflora, Shoots induction, BAP, NAA

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