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Acetylcholinesterase Immobilized on Glass Rod for Organophosphorus Pesticides Detection: Application on Milk Analysis

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@article{IJASEIT12910,
   author = {Asiya Utegenova and Zhainagul Kakimova and Alexey Klivenko and Zarina Kapshakbayeva and Galiya Imankulova and Gulmira Naurzbaeva and Gulnara Tulkebayeva and Gulmira Mirasheva},
   title = {Acetylcholinesterase Immobilized on Glass Rod for Organophosphorus Pesticides Detection: Application on Milk Analysis},
   journal = {International Journal on Advanced Science, Engineering and Information Technology},
   volume = {11},
   number = {3},
   year = {2021},
   pages = {843--848},
   keywords = {Analytical devices; food safety; glass rods; insecticide; malathion; milk analysis.},
   abstract = {Immense use of an insecticide such as malathion has initiated a hindrance to the environment and has increased possible risk to human health via milk and milk products. The presence of insecticide residues in milk products is an alarming signal to set up a string monitoring system. Since their risk to human health, the determination of organophosphate pesticide levels in dairy products is essential. This study shows biosensors' development for the detection of residues of the pesticides generally used in agriculture to increase productivity, which has high toxicity and selective action, a significant part of which falls into crop and livestock products. The test system described in this paper is based on the immobilization of acetylcholinesterase on glass rods by colorimetry as a detection technique. The application of the biosensor to the analysis of the real sample confirmed its reliability. The developed biosensor test system showed excellent analytical performance with limits to detecting the concentration range of residual amounts of the acetylcholinesterase enzyme inhibitor - malathion insecticide (up to 0.089 mg/kg), along with acceptable reproducibility and stability. Distinct advantages of enzyme-based biosensors are high sensitivity, convenience, quick response, cost-effectiveness, portability, and a possibility to use for environmental detection purposes. The biosensor can be used for the analysis of organophosphorus pesticides with anticholinesterase action. It can be concluded that the benefits of using immobilized enzymes are significant since it will bring advantages for the food, pharmaceutical, and medical device industries.},
   issn = {2088-5334},
   publisher = {INSIGHT - Indonesian Society for Knowledge and Human Development},
   url = {http://ijaseit.insightsociety.org/index.php?option=com_content&view=article&id=9&Itemid=1&article_id=12910},
   doi = {10.18517/ijaseit.11.3.12910}
}

EndNote

%A Utegenova, Asiya
%A Kakimova, Zhainagul
%A Klivenko, Alexey
%A Kapshakbayeva, Zarina
%A Imankulova, Galiya
%A Naurzbaeva, Gulmira
%A Tulkebayeva, Gulnara
%A Mirasheva, Gulmira
%D 2021
%T Acetylcholinesterase Immobilized on Glass Rod for Organophosphorus Pesticides Detection: Application on Milk Analysis
%B 2021
%9 Analytical devices; food safety; glass rods; insecticide; malathion; milk analysis.
%! Acetylcholinesterase Immobilized on Glass Rod for Organophosphorus Pesticides Detection: Application on Milk Analysis
%K Analytical devices; food safety; glass rods; insecticide; malathion; milk analysis.
%X Immense use of an insecticide such as malathion has initiated a hindrance to the environment and has increased possible risk to human health via milk and milk products. The presence of insecticide residues in milk products is an alarming signal to set up a string monitoring system. Since their risk to human health, the determination of organophosphate pesticide levels in dairy products is essential. This study shows biosensors' development for the detection of residues of the pesticides generally used in agriculture to increase productivity, which has high toxicity and selective action, a significant part of which falls into crop and livestock products. The test system described in this paper is based on the immobilization of acetylcholinesterase on glass rods by colorimetry as a detection technique. The application of the biosensor to the analysis of the real sample confirmed its reliability. The developed biosensor test system showed excellent analytical performance with limits to detecting the concentration range of residual amounts of the acetylcholinesterase enzyme inhibitor - malathion insecticide (up to 0.089 mg/kg), along with acceptable reproducibility and stability. Distinct advantages of enzyme-based biosensors are high sensitivity, convenience, quick response, cost-effectiveness, portability, and a possibility to use for environmental detection purposes. The biosensor can be used for the analysis of organophosphorus pesticides with anticholinesterase action. It can be concluded that the benefits of using immobilized enzymes are significant since it will bring advantages for the food, pharmaceutical, and medical device industries.
%U http://ijaseit.insightsociety.org/index.php?option=com_content&view=article&id=9&Itemid=1&article_id=12910
%R doi:10.18517/ijaseit.11.3.12910
%J International Journal on Advanced Science, Engineering and Information Technology
%V 11
%N 3
%@ 2088-5334

IEEE

Asiya Utegenova,Zhainagul Kakimova,Alexey Klivenko,Zarina Kapshakbayeva,Galiya Imankulova,Gulmira Naurzbaeva,Gulnara Tulkebayeva and Gulmira Mirasheva,"Acetylcholinesterase Immobilized on Glass Rod for Organophosphorus Pesticides Detection: Application on Milk Analysis," International Journal on Advanced Science, Engineering and Information Technology, vol. 11, no. 3, pp. 843-848, 2021. [Online]. Available: http://dx.doi.org/10.18517/ijaseit.11.3.12910.

RefMan/ProCite (RIS)

TY  - JOUR
AU  - Utegenova, Asiya
AU  - Kakimova, Zhainagul
AU  - Klivenko, Alexey
AU  - Kapshakbayeva, Zarina
AU  - Imankulova, Galiya
AU  - Naurzbaeva, Gulmira
AU  - Tulkebayeva, Gulnara
AU  - Mirasheva, Gulmira
PY  - 2021
TI  - Acetylcholinesterase Immobilized on Glass Rod for Organophosphorus Pesticides Detection: Application on Milk Analysis
JF  - International Journal on Advanced Science, Engineering and Information Technology; Vol. 11 (2021) No. 3
Y2  - 2021
SP  - 843
EP  - 848
SN  - 2088-5334
PB  - INSIGHT - Indonesian Society for Knowledge and Human Development
KW  - Analytical devices; food safety; glass rods; insecticide; malathion; milk analysis.
N2  - Immense use of an insecticide such as malathion has initiated a hindrance to the environment and has increased possible risk to human health via milk and milk products. The presence of insecticide residues in milk products is an alarming signal to set up a string monitoring system. Since their risk to human health, the determination of organophosphate pesticide levels in dairy products is essential. This study shows biosensors' development for the detection of residues of the pesticides generally used in agriculture to increase productivity, which has high toxicity and selective action, a significant part of which falls into crop and livestock products. The test system described in this paper is based on the immobilization of acetylcholinesterase on glass rods by colorimetry as a detection technique. The application of the biosensor to the analysis of the real sample confirmed its reliability. The developed biosensor test system showed excellent analytical performance with limits to detecting the concentration range of residual amounts of the acetylcholinesterase enzyme inhibitor - malathion insecticide (up to 0.089 mg/kg), along with acceptable reproducibility and stability. Distinct advantages of enzyme-based biosensors are high sensitivity, convenience, quick response, cost-effectiveness, portability, and a possibility to use for environmental detection purposes. The biosensor can be used for the analysis of organophosphorus pesticides with anticholinesterase action. It can be concluded that the benefits of using immobilized enzymes are significant since it will bring advantages for the food, pharmaceutical, and medical device industries.
UR  - http://ijaseit.insightsociety.org/index.php?option=com_content&view=article&id=9&Itemid=1&article_id=12910
DO  - 10.18517/ijaseit.11.3.12910

RefWorks

RT Journal Article
ID 12910
A1 Utegenova, Asiya
A1 Kakimova, Zhainagul
A1 Klivenko, Alexey
A1 Kapshakbayeva, Zarina
A1 Imankulova, Galiya
A1 Naurzbaeva, Gulmira
A1 Tulkebayeva, Gulnara
A1 Mirasheva, Gulmira
T1 Acetylcholinesterase Immobilized on Glass Rod for Organophosphorus Pesticides Detection: Application on Milk Analysis
JF International Journal on Advanced Science, Engineering and Information Technology
VO 11
IS 3
YR 2021
SP 843
OP 848
SN 2088-5334
PB INSIGHT - Indonesian Society for Knowledge and Human Development
K1 Analytical devices; food safety; glass rods; insecticide; malathion; milk analysis.
AB Immense use of an insecticide such as malathion has initiated a hindrance to the environment and has increased possible risk to human health via milk and milk products. The presence of insecticide residues in milk products is an alarming signal to set up a string monitoring system. Since their risk to human health, the determination of organophosphate pesticide levels in dairy products is essential. This study shows biosensors' development for the detection of residues of the pesticides generally used in agriculture to increase productivity, which has high toxicity and selective action, a significant part of which falls into crop and livestock products. The test system described in this paper is based on the immobilization of acetylcholinesterase on glass rods by colorimetry as a detection technique. The application of the biosensor to the analysis of the real sample confirmed its reliability. The developed biosensor test system showed excellent analytical performance with limits to detecting the concentration range of residual amounts of the acetylcholinesterase enzyme inhibitor - malathion insecticide (up to 0.089 mg/kg), along with acceptable reproducibility and stability. Distinct advantages of enzyme-based biosensors are high sensitivity, convenience, quick response, cost-effectiveness, portability, and a possibility to use for environmental detection purposes. The biosensor can be used for the analysis of organophosphorus pesticides with anticholinesterase action. It can be concluded that the benefits of using immobilized enzymes are significant since it will bring advantages for the food, pharmaceutical, and medical device industries.
LK http://ijaseit.insightsociety.org/index.php?option=com_content&view=article&id=9&Itemid=1&article_id=12910
DO  - 10.18517/ijaseit.11.3.12910