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DNA Isolation and Optimization of ISSR-PCR Reaction System in Oryza sativa L.

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@article{IJASEIT1621,
   author = {Azhar Mohamad and Arshad Naji Alhasnawi and Ahsan A. Kadhimi and Anizan Isahak and Wan Mohtar Wan Yusoff and C.M.Z Che Radziah},
   title = {DNA Isolation and Optimization of ISSR-PCR Reaction System in Oryza sativa L.},
   journal = {International Journal on Advanced Science, Engineering and Information Technology},
   volume = {7},
   number = {6},
   year = {2017},
   pages = {2264--2272},
   keywords = {DNA isolation; optimal PCR condition; ISSR screening; plant genetics.},
   abstract = {Inter simple sequence repeats (ISSRs) have been utilized widely for molecular markers in analyzing the genetic diversity and phylogenetic and regions in the genome flanked by microsatellite sequences. PCR amplification of these regions using a single primer yields multiple amplification products that can be used as a dominant multilocus marker system for the study of genetic variation in various organisms. For this study provides, DNA isolation, adjusting in six factors (Buffer, MgCl2, dNTPs, ISSR primers, Template DNA and Taq polymerase) at six levels, and optimization of PCR temperature for the ISSR reaction was 60-45 °C, primers screening on indica rice (Oryza sativa). In this research, simple method of DNA isolation by using seedling. The objective of the present investigation was to assess the optimizations and quantification. Has been shown that stalk enhanced the maximum value of genomic. The results show that 100 ISSR primers were examined as well as, 56 ISSR primers was productively amplified. Optimum components for PCR reactions were 5.0 μl of 5X PCR Buffer, 1.5 μl of 25mM MgCl2, 1 μl of 10 mM dNTP, 1 μl of 10 Μm ISSR primers, 2 μl Template DNA, and 0.1 μl of 5 units/ml Taq polymerase. Based on this study, has brought out some information on the relationship between these ISSR primers will be applied further for molecular profiling as well as response evaluation in rice varieties},
   issn = {2088-5334},
   publisher = {INSIGHT - Indonesian Society for Knowledge and Human Development},
   url = {http://ijaseit.insightsociety.org/index.php?option=com_content&view=article&id=9&Itemid=1&article_id=1621},
   doi = {10.18517/ijaseit.7.6.1621}
}

EndNote

%A Mohamad, Azhar
%A Alhasnawi, Arshad Naji
%A Kadhimi, Ahsan A.
%A Isahak, Anizan
%A Wan Yusoff, Wan Mohtar
%A Che Radziah, C.M.Z
%D 2017
%T DNA Isolation and Optimization of ISSR-PCR Reaction System in Oryza sativa L.
%B 2017
%9 DNA isolation; optimal PCR condition; ISSR screening; plant genetics.
%! DNA Isolation and Optimization of ISSR-PCR Reaction System in Oryza sativa L.
%K DNA isolation; optimal PCR condition; ISSR screening; plant genetics.
%X Inter simple sequence repeats (ISSRs) have been utilized widely for molecular markers in analyzing the genetic diversity and phylogenetic and regions in the genome flanked by microsatellite sequences. PCR amplification of these regions using a single primer yields multiple amplification products that can be used as a dominant multilocus marker system for the study of genetic variation in various organisms. For this study provides, DNA isolation, adjusting in six factors (Buffer, MgCl2, dNTPs, ISSR primers, Template DNA and Taq polymerase) at six levels, and optimization of PCR temperature for the ISSR reaction was 60-45 °C, primers screening on indica rice (Oryza sativa). In this research, simple method of DNA isolation by using seedling. The objective of the present investigation was to assess the optimizations and quantification. Has been shown that stalk enhanced the maximum value of genomic. The results show that 100 ISSR primers were examined as well as, 56 ISSR primers was productively amplified. Optimum components for PCR reactions were 5.0 μl of 5X PCR Buffer, 1.5 μl of 25mM MgCl2, 1 μl of 10 mM dNTP, 1 μl of 10 Μm ISSR primers, 2 μl Template DNA, and 0.1 μl of 5 units/ml Taq polymerase. Based on this study, has brought out some information on the relationship between these ISSR primers will be applied further for molecular profiling as well as response evaluation in rice varieties
%U http://ijaseit.insightsociety.org/index.php?option=com_content&view=article&id=9&Itemid=1&article_id=1621
%R doi:10.18517/ijaseit.7.6.1621
%J International Journal on Advanced Science, Engineering and Information Technology
%V 7
%N 6
%@ 2088-5334

IEEE

Azhar Mohamad,Arshad Naji Alhasnawi,Ahsan A. Kadhimi,Anizan Isahak,Wan Mohtar Wan Yusoff and C.M.Z Che Radziah,"DNA Isolation and Optimization of ISSR-PCR Reaction System in Oryza sativa L.," International Journal on Advanced Science, Engineering and Information Technology, vol. 7, no. 6, pp. 2264-2272, 2017. [Online]. Available: http://dx.doi.org/10.18517/ijaseit.7.6.1621.

RefMan/ProCite (RIS)

TY  - JOUR
AU  - Mohamad, Azhar
AU  - Alhasnawi, Arshad Naji
AU  - Kadhimi, Ahsan A.
AU  - Isahak, Anizan
AU  - Wan Yusoff, Wan Mohtar
AU  - Che Radziah, C.M.Z
PY  - 2017
TI  - DNA Isolation and Optimization of ISSR-PCR Reaction System in Oryza sativa L.
JF  - International Journal on Advanced Science, Engineering and Information Technology; Vol. 7 (2017) No. 6
Y2  - 2017
SP  - 2264
EP  - 2272
SN  - 2088-5334
PB  - INSIGHT - Indonesian Society for Knowledge and Human Development
KW  - DNA isolation; optimal PCR condition; ISSR screening; plant genetics.
N2  - Inter simple sequence repeats (ISSRs) have been utilized widely for molecular markers in analyzing the genetic diversity and phylogenetic and regions in the genome flanked by microsatellite sequences. PCR amplification of these regions using a single primer yields multiple amplification products that can be used as a dominant multilocus marker system for the study of genetic variation in various organisms. For this study provides, DNA isolation, adjusting in six factors (Buffer, MgCl2, dNTPs, ISSR primers, Template DNA and Taq polymerase) at six levels, and optimization of PCR temperature for the ISSR reaction was 60-45 °C, primers screening on indica rice (Oryza sativa). In this research, simple method of DNA isolation by using seedling. The objective of the present investigation was to assess the optimizations and quantification. Has been shown that stalk enhanced the maximum value of genomic. The results show that 100 ISSR primers were examined as well as, 56 ISSR primers was productively amplified. Optimum components for PCR reactions were 5.0 μl of 5X PCR Buffer, 1.5 μl of 25mM MgCl2, 1 μl of 10 mM dNTP, 1 μl of 10 Μm ISSR primers, 2 μl Template DNA, and 0.1 μl of 5 units/ml Taq polymerase. Based on this study, has brought out some information on the relationship between these ISSR primers will be applied further for molecular profiling as well as response evaluation in rice varieties
UR  - http://ijaseit.insightsociety.org/index.php?option=com_content&view=article&id=9&Itemid=1&article_id=1621
DO  - 10.18517/ijaseit.7.6.1621

RefWorks

RT Journal Article
ID 1621
A1 Mohamad, Azhar
A1 Alhasnawi, Arshad Naji
A1 Kadhimi, Ahsan A.
A1 Isahak, Anizan
A1 Wan Yusoff, Wan Mohtar
A1 Che Radziah, C.M.Z
T1 DNA Isolation and Optimization of ISSR-PCR Reaction System in Oryza sativa L.
JF International Journal on Advanced Science, Engineering and Information Technology
VO 7
IS 6
YR 2017
SP 2264
OP 2272
SN 2088-5334
PB INSIGHT - Indonesian Society for Knowledge and Human Development
K1 DNA isolation; optimal PCR condition; ISSR screening; plant genetics.
AB Inter simple sequence repeats (ISSRs) have been utilized widely for molecular markers in analyzing the genetic diversity and phylogenetic and regions in the genome flanked by microsatellite sequences. PCR amplification of these regions using a single primer yields multiple amplification products that can be used as a dominant multilocus marker system for the study of genetic variation in various organisms. For this study provides, DNA isolation, adjusting in six factors (Buffer, MgCl2, dNTPs, ISSR primers, Template DNA and Taq polymerase) at six levels, and optimization of PCR temperature for the ISSR reaction was 60-45 °C, primers screening on indica rice (Oryza sativa). In this research, simple method of DNA isolation by using seedling. The objective of the present investigation was to assess the optimizations and quantification. Has been shown that stalk enhanced the maximum value of genomic. The results show that 100 ISSR primers were examined as well as, 56 ISSR primers was productively amplified. Optimum components for PCR reactions were 5.0 μl of 5X PCR Buffer, 1.5 μl of 25mM MgCl2, 1 μl of 10 mM dNTP, 1 μl of 10 Μm ISSR primers, 2 μl Template DNA, and 0.1 μl of 5 units/ml Taq polymerase. Based on this study, has brought out some information on the relationship between these ISSR primers will be applied further for molecular profiling as well as response evaluation in rice varieties
LK http://ijaseit.insightsociety.org/index.php?option=com_content&view=article&id=9&Itemid=1&article_id=1621
DO  - 10.18517/ijaseit.7.6.1621