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Construction and Expression of L-Arabinose Isomerase (L-AI) in Cell- Surface of Pichia pastoris

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@article{IJASEIT863,
   author = {Agnes Yuliana and - Hariyatun and Asrul Muhammad Fuad and Antonius Suwanto and Wien Kusharyoto},
   title = {Construction and Expression of L-Arabinose Isomerase (L-AI) in Cell- Surface of Pichia pastoris},
   journal = {International Journal on Advanced Science, Engineering and Information Technology},
   volume = {6},
   number = {5},
   year = {2016},
   pages = {649--656},
   keywords = {araA; L-Arabinose isomerase; Yeast surface display; Pichia pastoris},
   abstract = {araA gene encode L-arabinose isomerase (L-AI). It is an enzyme converting D-galactose to D-tagatose. D-tagatose is is a hexoketose monosaccharide sweetener, which is an isomer of D-galactose and rarely found in nature. It is a potential sweetener which has low calorie. The aim of this study is to construct araA gene in the expression vector pJ912-AGα and expression the protein in the cell-surface of Pichia pastoris GS115. Both vector pJ912-AGα and araA gene was digested with SalI and Kpn2I restriction enzymes then was ligated. The ligation solution had been successfully introduced into Escherichia coli DH5α. Vektor pJ912-AGα-araA was successfully integrated into the genome of P. pastoris  GS115. Genetically stable transformed cells have been obtained after selection on zeocin medium up to 1000 μg/mL zeocin. We had successfully syntheized L-AI protein in the P. pastoris GS115. Observation using fluorescence microscopy has proven that successful transformaned cell emit green fluorescence derived from the interaction of functional His6 protein and rabbit polyclonal to 6×His tag® and showed that L-AI protein was expressed successfully in cell-surface of P.pastoris.},
   issn = {2088-5334},
   publisher = {INSIGHT - Indonesian Society for Knowledge and Human Development},
   url = {http://ijaseit.insightsociety.org/index.php?option=com_content&view=article&id=9&Itemid=1&article_id=863},
   doi = {10.18517/ijaseit.6.5.863}
}

EndNote

%A Yuliana, Agnes
%A Hariyatun, -
%A Fuad, Asrul Muhammad
%A Suwanto, Antonius
%A Kusharyoto, Wien
%D 2016
%T Construction and Expression of L-Arabinose Isomerase (L-AI) in Cell- Surface of Pichia pastoris
%B 2016
%9 araA; L-Arabinose isomerase; Yeast surface display; Pichia pastoris
%! Construction and Expression of L-Arabinose Isomerase (L-AI) in Cell- Surface of Pichia pastoris
%K araA; L-Arabinose isomerase; Yeast surface display; Pichia pastoris
%X araA gene encode L-arabinose isomerase (L-AI). It is an enzyme converting D-galactose to D-tagatose. D-tagatose is is a hexoketose monosaccharide sweetener, which is an isomer of D-galactose and rarely found in nature. It is a potential sweetener which has low calorie. The aim of this study is to construct araA gene in the expression vector pJ912-AGα and expression the protein in the cell-surface of Pichia pastoris GS115. Both vector pJ912-AGα and araA gene was digested with SalI and Kpn2I restriction enzymes then was ligated. The ligation solution had been successfully introduced into Escherichia coli DH5α. Vektor pJ912-AGα-araA was successfully integrated into the genome of P. pastoris  GS115. Genetically stable transformed cells have been obtained after selection on zeocin medium up to 1000 μg/mL zeocin. We had successfully syntheized L-AI protein in the P. pastoris GS115. Observation using fluorescence microscopy has proven that successful transformaned cell emit green fluorescence derived from the interaction of functional His6 protein and rabbit polyclonal to 6×His tag® and showed that L-AI protein was expressed successfully in cell-surface of P.pastoris.
%U http://ijaseit.insightsociety.org/index.php?option=com_content&view=article&id=9&Itemid=1&article_id=863
%R doi:10.18517/ijaseit.6.5.863
%J International Journal on Advanced Science, Engineering and Information Technology
%V 6
%N 5
%@ 2088-5334

IEEE

Agnes Yuliana,- Hariyatun,Asrul Muhammad Fuad,Antonius Suwanto and Wien Kusharyoto,"Construction and Expression of L-Arabinose Isomerase (L-AI) in Cell- Surface of Pichia pastoris," International Journal on Advanced Science, Engineering and Information Technology, vol. 6, no. 5, pp. 649-656, 2016. [Online]. Available: http://dx.doi.org/10.18517/ijaseit.6.5.863.

RefMan/ProCite (RIS)

TY  - JOUR
AU  - Yuliana, Agnes
AU  - Hariyatun, -
AU  - Fuad, Asrul Muhammad
AU  - Suwanto, Antonius
AU  - Kusharyoto, Wien
PY  - 2016
TI  - Construction and Expression of L-Arabinose Isomerase (L-AI) in Cell- Surface of Pichia pastoris
JF  - International Journal on Advanced Science, Engineering and Information Technology; Vol. 6 (2016) No. 5
Y2  - 2016
SP  - 649
EP  - 656
SN  - 2088-5334
PB  - INSIGHT - Indonesian Society for Knowledge and Human Development
KW  - araA; L-Arabinose isomerase; Yeast surface display; Pichia pastoris
N2  - araA gene encode L-arabinose isomerase (L-AI). It is an enzyme converting D-galactose to D-tagatose. D-tagatose is is a hexoketose monosaccharide sweetener, which is an isomer of D-galactose and rarely found in nature. It is a potential sweetener which has low calorie. The aim of this study is to construct araA gene in the expression vector pJ912-AGα and expression the protein in the cell-surface of Pichia pastoris GS115. Both vector pJ912-AGα and araA gene was digested with SalI and Kpn2I restriction enzymes then was ligated. The ligation solution had been successfully introduced into Escherichia coli DH5α. Vektor pJ912-AGα-araA was successfully integrated into the genome of P. pastoris  GS115. Genetically stable transformed cells have been obtained after selection on zeocin medium up to 1000 μg/mL zeocin. We had successfully syntheized L-AI protein in the P. pastoris GS115. Observation using fluorescence microscopy has proven that successful transformaned cell emit green fluorescence derived from the interaction of functional His6 protein and rabbit polyclonal to 6×His tag® and showed that L-AI protein was expressed successfully in cell-surface of P.pastoris.
UR  - http://ijaseit.insightsociety.org/index.php?option=com_content&view=article&id=9&Itemid=1&article_id=863
DO  - 10.18517/ijaseit.6.5.863

RefWorks

RT Journal Article
ID 863
A1 Yuliana, Agnes
A1 Hariyatun, -
A1 Fuad, Asrul Muhammad
A1 Suwanto, Antonius
A1 Kusharyoto, Wien
T1 Construction and Expression of L-Arabinose Isomerase (L-AI) in Cell- Surface of Pichia pastoris
JF International Journal on Advanced Science, Engineering and Information Technology
VO 6
IS 5
YR 2016
SP 649
OP 656
SN 2088-5334
PB INSIGHT - Indonesian Society for Knowledge and Human Development
K1 araA; L-Arabinose isomerase; Yeast surface display; Pichia pastoris
AB araA gene encode L-arabinose isomerase (L-AI). It is an enzyme converting D-galactose to D-tagatose. D-tagatose is is a hexoketose monosaccharide sweetener, which is an isomer of D-galactose and rarely found in nature. It is a potential sweetener which has low calorie. The aim of this study is to construct araA gene in the expression vector pJ912-AGα and expression the protein in the cell-surface of Pichia pastoris GS115. Both vector pJ912-AGα and araA gene was digested with SalI and Kpn2I restriction enzymes then was ligated. The ligation solution had been successfully introduced into Escherichia coli DH5α. Vektor pJ912-AGα-araA was successfully integrated into the genome of P. pastoris  GS115. Genetically stable transformed cells have been obtained after selection on zeocin medium up to 1000 μg/mL zeocin. We had successfully syntheized L-AI protein in the P. pastoris GS115. Observation using fluorescence microscopy has proven that successful transformaned cell emit green fluorescence derived from the interaction of functional His6 protein and rabbit polyclonal to 6×His tag® and showed that L-AI protein was expressed successfully in cell-surface of P.pastoris.
LK http://ijaseit.insightsociety.org/index.php?option=com_content&view=article&id=9&Itemid=1&article_id=863
DO  - 10.18517/ijaseit.6.5.863