International Journal on Advanced Science, Engineering and Information Technology, Vol. 4 (2014) No. 3, pages: 196-200, DOI:10.18517/ijaseit.4.3.399

Characterization of Indigenous Rhizobacterial Isolates from Healthy Chilli Rhizosphere Capable of Inducing Resistance Against Anthracnose Disease (Colletotrichum Gloeosporioides).

Fatimah Fatimah, T. Habazar, D. Arbain, Nurbailis Nurbailis

Abstract

Antrachnose disease on chilli  caused by Colletotrichum gloeosporioides is difficult to be controlled because the disease can be transmitted through the seeds, and has a high genetic diversity. One of promising alternative control is using biological control agents, such as groups of rhizobacteria. The objective of this research were : to characterize the morphology, physiology and molecular of  selected rhizobacterial isolates,  which were capable of controlling the anthracnose disease  and to enhance the growth and yield chilli. Three  rhizobacterial isolates (B1.37, B2.11 and P1.31) were used. These isolates were indentified based on morphology (colony form, elevation, edge, and color), physiology (gram tes, the production of hormone IAA, chitinase enzyme, hydrogen cyanide, and solvents phosphate) and molecular.  The isolates were identified by using  16S rRNA sequencing.  The results indicated that isolate B1.37 belonged to species of Bacillus cereus strain ML 267, isolate B2.11 belonged to Bacillus cereus strain LH8 and isolate P1.31 belonged to  Chryseobacterium gleum strain NBRC 15054.

Keywords:

Morphology, physiology, molecular, rhizobacteria, anthracnose, chilli

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