International Journal on Advanced Science, Engineering and Information Technology, Vol. 5 (2015) No. 1, pages: 27-30, DOI:10.18517/ijaseit.5.1.480

Screening of Rhizosphere Bacteria From Rice Fields in The Coastal Area as Acc-Deaminase and Auxin Producer

Annisyia Zarina Putri, Djarot Sasongko, Dwi N Susilowati

Abstract

Salinity and drought stress results in the production of ethylene. Rhizosphere bacterial activity suppresses the production of ethylene through the activity of 1-aminocycopropane-1-carboxylate (ACC) deaminase. In this study,a sampel of rhizosphere bacteria from coastal rice plant area was tested. The method used was acc deaminase activity test performed on Dworkin - Foster (DF) media and PCR acdS gene using specific primers of ACC and a quantitative analysis of the production of auxin (IAA). Of 47 isolatees obtained, 8 were positively have acdS activity. The positive result was indicated by the presence of 1080 bp amplicon. Quantitative analysisshowed the highest yield of 10.6 ppm of auxin. The results prove that there are some bacteria originated from coastal rice plant area which have acc deaminase activity.

Keywords:

ACC deaminase, auxin, drought, salinity, rhizosfere bacteria, rice

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