International Journal on Advanced Science, Engineering and Information Technology, Vol. 9 (2019) No. 2, List of accepted papers, DOI:10.18517/ijaseit.9.2.8297

The Optimization of Temperature and Length of Extractionof Local Corn Silk Powder Using Response Surface Methodology

Haslina Haslina

Abstract

Corn silk from female flowers consists of a group of stigmas that are smooth, soft, and look like threads and yellowish silk. Corn silk is rich in phenolic compounds, especially flavonoids and high natural antioxidant compounds. This content varies according to the corn varieties. Bisma variety is one of the many local corn varieties grown in Temanggung, Central Java. The first step in separating phenolic compounds is extraction with solvents. Methanol is one of the solvents that are often used in the extraction of phenolic compounds. Some of the contributing factors affect the rate and quality of extraction on the components of bioactive compounds, two of which are temperature and length of extraction.  This study aims to determine the optimum condition of extraction of corn silk from local Bisma varieties using Response Surface Methodology. The experimental design used was factorial in the Randomized Block Design (RBD) with 2 factors and 3 replications. The local corn silk of the Bisma variety, 80% methanol solvent concentration, and a ratio (1:20) were extracted with a length of extraction (60; 70; 80; and 90) minutes and a temperature of 40; 50; 60; and 70°C. The extraction results from each corn silk were analyzed for chemical and phytochemical characteristics. The optimum condition for extracting corn silk powder was obtained at 40oC and the extraction time was 60 minutes. In this condition,  chemical content produced are as follow: 0.80% of soluble fiber, 0.58% of insoluble fiber, 1.38% of total fiber, 2.36% of moisture content, 2.50% of ash content, 1.29% of fat content, protein content of 16.93%, and carbohydrate content of 78.20%, and phytochemical content were  total phenol of 5342.45µg EAG/g, total flavonoids  of 167.45µg EAG/g, beta sitosterol of 996.53 ppm, and antioxidant activity of 50.41 %.

                                                                                                                                                                                                   

Keywords:

corn silk, extraction, dietary fiber, phytochemicals, proximate, Response Surface Methodology

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