International Journal on Advanced Science, Engineering and Information Technology, Vol. 7 (2017) No. 3, pages: 904-909, DOI:10.18517/ijaseit.7.3.1003

Optimal Media for In-Vitro Regeneration of Two Local Genotypes of Chili Pepper (Capsicum annuum L.) from West Sumatera

- Renfiyeni, Lily Syukriani, Alfi Asben, - Jamsari

Abstract

Genetic engineering strategy in chilli pepper (Capsicum annuum L.) has been applied so far in order to improve its genetic capacity for instance against some important diseases. This strategy needs an established system for calli preparation as well as transformant regeneration. In most cases, this necessity is genotype dependent especially for some local genotypes. Based on this, we optimized media for calli induction, shoot and root regeneration of our three local cultivars namely Kampung, Kopay and Trisula. Media combinations basically were composed of MS  and combined with some growth factors for instance IAA, BAP, NAA and TDZ with different combinations for each calli induction, shoot and root regeneration. All media compositions were supplemented with 30 GL-1 sucrose. The best medium for calli induction was MS media supplemented with 4 mgL-1 BAP, 0.5 mgL-1 IAA and L2 vitamin (Thiamin-HCl and Pyrodoxine-HCl). While the best medium for shoot induction and elongation was MS supplemented with 1mgL-1 BAP, 5 mgL-1 AgNO3, 2 mgL-1 GA3, 2 mgL-1 Calcium Pantotenate and L2 vitamin.  Furthermore, the best medium for root induction and elongation was WPM (Woody Plant Medium) supplemented with 0.4 mgL-1 NAA. This finding should provide optimal media for calli induction and shoot-root regeneration for the three local cultivars Kampung, Kopay and Trisula.

Keywords:

calli induction; shoot and root induction; Capsicum annuum; genetic transformation; local genotype

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