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Screening and Enzyme Activity of Cellulase-Producing Bacteria Isolated from Kemiri Sunan (Reutealis trisperma (Blanco) Airy Shaw) and Empty Fruit Bunches of the Palm Oil (Elaieis guineensis)

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@article{IJASEIT11314,
   author = {Tri Yuliana and Efri Mardawati and Emilda A. Febrianti and Agus T. Hartono and Souvia Rahimah},
   title = {Screening and Enzyme Activity of Cellulase-Producing Bacteria Isolated from Kemiri Sunan (Reutealis trisperma (Blanco) Airy Shaw) and Empty Fruit Bunches of the Palm Oil (Elaieis guineensis)},
   journal = {International Journal on Advanced Science, Engineering and Information Technology},
   volume = {11},
   number = {1},
   year = {2021},
   pages = {191--196},
   keywords = {Kemiri Sunan; cellulose; screening; enzyme activity; palm oil.},
   abstract = {

Cellulase enzymes have an important role in biocatalyst technology, especially in the pulp industry. Cellulase is synthesized by a large type of microorganisms, including cellulose-producing bacteria isolated from Kemiri Sunan (Reutealis trisperma) shell and empty fruit bunches of the palm oil (EFBPO). In this study, the screening of cellulase-producing bacteria isolated from Kemiri Sunan shell and EFBPO were carried out in order to evaluate its enzyme activity. The method used was an experimental method followed by descriptive analysis using four treatments with two repetitions. This study includes; (1) Lignocellulosic biomass analysis, (2) Isolation of cellulase-producing bacteria, (3) Qualitative assay of cellulase-producing bacteria, (4) Enzyme activity assay. Both cellulose and hemicellulose contents in Kemiri Sunan shell were 27.38% and 44.46%, respectively, and 48.55% and 28.06% in empty fruit bunches of palm oil.  Qualitative assay of cellulase-producing bacteria from Kemiri Sunan shell resulted in the widest clear zone accounting of 77.19% (isolate K2) and 73.06% (isolate T3) from EFBPO. Screening continued by measuring the cell growth with an interval time of 8, 24, 32 and 48 h. The enzyme activity showed the highest cellulase activity (isolate K3) from Kemiri Sunan shell was 4.3 × 10-2 U/mL after 48 h incubation. However, isolate T4 from EFBPO resulted in higher cellulase activity than that Kemiri Sunan shell, accounting of 7.2 × 10-2 U/mL. Our study suggests that cellulase-producing bacteria from Kemiri Sunan and EFBPO has been screened to synthesize cellulase enzyme.

},    issn = {2088-5334},    publisher = {INSIGHT - Indonesian Society for Knowledge and Human Development},    url = {http://ijaseit.insightsociety.org/index.php?option=com_content&view=article&id=9&Itemid=1&article_id=11314},    doi = {10.18517/ijaseit.11.1.11314} }

EndNote

%A Yuliana, Tri
%A Mardawati, Efri
%A Febrianti, Emilda A.
%A Hartono, Agus T.
%A Rahimah, Souvia
%D 2021
%T Screening and Enzyme Activity of Cellulase-Producing Bacteria Isolated from Kemiri Sunan (Reutealis trisperma (Blanco) Airy Shaw) and Empty Fruit Bunches of the Palm Oil (Elaieis guineensis)
%B 2021
%9 Kemiri Sunan; cellulose; screening; enzyme activity; palm oil.
%! Screening and Enzyme Activity of Cellulase-Producing Bacteria Isolated from Kemiri Sunan (Reutealis trisperma (Blanco) Airy Shaw) and Empty Fruit Bunches of the Palm Oil (Elaieis guineensis)
%K Kemiri Sunan; cellulose; screening; enzyme activity; palm oil.
%X 

Cellulase enzymes have an important role in biocatalyst technology, especially in the pulp industry. Cellulase is synthesized by a large type of microorganisms, including cellulose-producing bacteria isolated from Kemiri Sunan (Reutealis trisperma) shell and empty fruit bunches of the palm oil (EFBPO). In this study, the screening of cellulase-producing bacteria isolated from Kemiri Sunan shell and EFBPO were carried out in order to evaluate its enzyme activity. The method used was an experimental method followed by descriptive analysis using four treatments with two repetitions. This study includes; (1) Lignocellulosic biomass analysis, (2) Isolation of cellulase-producing bacteria, (3) Qualitative assay of cellulase-producing bacteria, (4) Enzyme activity assay. Both cellulose and hemicellulose contents in Kemiri Sunan shell were 27.38% and 44.46%, respectively, and 48.55% and 28.06% in empty fruit bunches of palm oil.  Qualitative assay of cellulase-producing bacteria from Kemiri Sunan shell resulted in the widest clear zone accounting of 77.19% (isolate K2) and 73.06% (isolate T3) from EFBPO. Screening continued by measuring the cell growth with an interval time of 8, 24, 32 and 48 h. The enzyme activity showed the highest cellulase activity (isolate K3) from Kemiri Sunan shell was 4.3 × 10-2 U/mL after 48 h incubation. However, isolate T4 from EFBPO resulted in higher cellulase activity than that Kemiri Sunan shell, accounting of 7.2 × 10-2 U/mL. Our study suggests that cellulase-producing bacteria from Kemiri Sunan and EFBPO has been screened to synthesize cellulase enzyme.

%U http://ijaseit.insightsociety.org/index.php?option=com_content&view=article&id=9&Itemid=1&article_id=11314 %R doi:10.18517/ijaseit.11.1.11314 %J International Journal on Advanced Science, Engineering and Information Technology %V 11 %N 1 %@ 2088-5334

IEEE

Tri Yuliana,Efri Mardawati,Emilda A. Febrianti,Agus T. Hartono and Souvia Rahimah,"Screening and Enzyme Activity of Cellulase-Producing Bacteria Isolated from Kemiri Sunan (Reutealis trisperma (Blanco) Airy Shaw) and Empty Fruit Bunches of the Palm Oil (Elaieis guineensis)," International Journal on Advanced Science, Engineering and Information Technology, vol. 11, no. 1, pp. 191-196, 2021. [Online]. Available: http://dx.doi.org/10.18517/ijaseit.11.1.11314.

RefMan/ProCite (RIS)

TY  - JOUR
AU  - Yuliana, Tri
AU  - Mardawati, Efri
AU  - Febrianti, Emilda A.
AU  - Hartono, Agus T.
AU  - Rahimah, Souvia
PY  - 2021
TI  - Screening and Enzyme Activity of Cellulase-Producing Bacteria Isolated from Kemiri Sunan (Reutealis trisperma (Blanco) Airy Shaw) and Empty Fruit Bunches of the Palm Oil (Elaieis guineensis)
JF  - International Journal on Advanced Science, Engineering and Information Technology; Vol. 11 (2021) No. 1
Y2  - 2021
SP  - 191
EP  - 196
SN  - 2088-5334
PB  - INSIGHT - Indonesian Society for Knowledge and Human Development
KW  - Kemiri Sunan; cellulose; screening; enzyme activity; palm oil.
N2  - 

Cellulase enzymes have an important role in biocatalyst technology, especially in the pulp industry. Cellulase is synthesized by a large type of microorganisms, including cellulose-producing bacteria isolated from Kemiri Sunan (Reutealis trisperma) shell and empty fruit bunches of the palm oil (EFBPO). In this study, the screening of cellulase-producing bacteria isolated from Kemiri Sunan shell and EFBPO were carried out in order to evaluate its enzyme activity. The method used was an experimental method followed by descriptive analysis using four treatments with two repetitions. This study includes; (1) Lignocellulosic biomass analysis, (2) Isolation of cellulase-producing bacteria, (3) Qualitative assay of cellulase-producing bacteria, (4) Enzyme activity assay. Both cellulose and hemicellulose contents in Kemiri Sunan shell were 27.38% and 44.46%, respectively, and 48.55% and 28.06% in empty fruit bunches of palm oil.  Qualitative assay of cellulase-producing bacteria from Kemiri Sunan shell resulted in the widest clear zone accounting of 77.19% (isolate K2) and 73.06% (isolate T3) from EFBPO. Screening continued by measuring the cell growth with an interval time of 8, 24, 32 and 48 h. The enzyme activity showed the highest cellulase activity (isolate K3) from Kemiri Sunan shell was 4.3 × 10-2 U/mL after 48 h incubation. However, isolate T4 from EFBPO resulted in higher cellulase activity than that Kemiri Sunan shell, accounting of 7.2 × 10-2 U/mL. Our study suggests that cellulase-producing bacteria from Kemiri Sunan and EFBPO has been screened to synthesize cellulase enzyme.

UR - http://ijaseit.insightsociety.org/index.php?option=com_content&view=article&id=9&Itemid=1&article_id=11314 DO - 10.18517/ijaseit.11.1.11314

RefWorks

RT Journal Article
ID 11314
A1 Yuliana, Tri
A1 Mardawati, Efri
A1 Febrianti, Emilda A.
A1 Hartono, Agus T.
A1 Rahimah, Souvia
T1 Screening and Enzyme Activity of Cellulase-Producing Bacteria Isolated from Kemiri Sunan (Reutealis trisperma (Blanco) Airy Shaw) and Empty Fruit Bunches of the Palm Oil (Elaieis guineensis)
JF International Journal on Advanced Science, Engineering and Information Technology
VO 11
IS 1
YR 2021
SP 191
OP 196
SN 2088-5334
PB INSIGHT - Indonesian Society for Knowledge and Human Development
K1 Kemiri Sunan; cellulose; screening; enzyme activity; palm oil.
AB 

Cellulase enzymes have an important role in biocatalyst technology, especially in the pulp industry. Cellulase is synthesized by a large type of microorganisms, including cellulose-producing bacteria isolated from Kemiri Sunan (Reutealis trisperma) shell and empty fruit bunches of the palm oil (EFBPO). In this study, the screening of cellulase-producing bacteria isolated from Kemiri Sunan shell and EFBPO were carried out in order to evaluate its enzyme activity. The method used was an experimental method followed by descriptive analysis using four treatments with two repetitions. This study includes; (1) Lignocellulosic biomass analysis, (2) Isolation of cellulase-producing bacteria, (3) Qualitative assay of cellulase-producing bacteria, (4) Enzyme activity assay. Both cellulose and hemicellulose contents in Kemiri Sunan shell were 27.38% and 44.46%, respectively, and 48.55% and 28.06% in empty fruit bunches of palm oil.  Qualitative assay of cellulase-producing bacteria from Kemiri Sunan shell resulted in the widest clear zone accounting of 77.19% (isolate K2) and 73.06% (isolate T3) from EFBPO. Screening continued by measuring the cell growth with an interval time of 8, 24, 32 and 48 h. The enzyme activity showed the highest cellulase activity (isolate K3) from Kemiri Sunan shell was 4.3 × 10-2 U/mL after 48 h incubation. However, isolate T4 from EFBPO resulted in higher cellulase activity than that Kemiri Sunan shell, accounting of 7.2 × 10-2 U/mL. Our study suggests that cellulase-producing bacteria from Kemiri Sunan and EFBPO has been screened to synthesize cellulase enzyme.

LK http://ijaseit.insightsociety.org/index.php?option=com_content&view=article&id=9&Itemid=1&article_id=11314 DO - 10.18517/ijaseit.11.1.11314