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Synthesis of Antioxidant Peptides from Melinjo (Gnetum gnemon) Seed Protein Isolated Using Sol-Gel Immobilized Alcalase

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@article{IJASEIT936,
   author = {Tri Agus Siswoyo and Nur Fauziah Matra and Anadini Aulia Safiera and Anang Supriyadi},
   title = {Synthesis of Antioxidant Peptides from Melinjo (Gnetum gnemon) Seed Protein Isolated Using Sol-Gel Immobilized Alcalase},
   journal = {International Journal on Advanced Science, Engineering and Information Technology},
   volume = {7},
   number = {4},
   year = {2017},
   pages = {1315--1321},
   keywords = {melinjo; immobilization; alcalase; antioxidant; peptide; Gnetum gnemon},
   abstract = {

The synthetic of antioxidant peptides from melinjo (Gnetum gnemon) seed protein isolated using sol-gel immobilized alcalase was studied, a commercial alcalase was physically entrapped in sol-gel matrices system. Characteristics of protein hydrolyzed were evaluated by the degree of hydrolysis obtained (DH) and the peptide profile by FPLC and SDS-PAGE. The antioxidant activity of protein hydrolyzed was determined using ABTS cation, DPPH, and superoxide radical scavenging assays. The DH values increased very quickly during the second hour followed by a slow increase up to 12 hours. Melinjo seed protein isolated was hydrolyzed about 23% of degree hydrolysis at 50°C after 2 hours of incubation. Similar results were also obtained by FPLC and SDS-PAGE, the peptide profile indicated the sol-gel immobilized alcalase was produced of peptides with low molecular weight from melinjo seed protein isolated. Immobilized alcalase preserved the initial activity over 4 cycles of repeated use in a routine reaction. The ABTS assay, the hydrolysate presented a more than  5 times greater activity than non-hydrolyzed melinjo seed protein isolated. It's confirming the potential use of sol-gel immobilized alcalase in synthetic antioxidant peptides from melinjo seed protein isolated. 

},    issn = {2088-5334},    publisher = {INSIGHT - Indonesian Society for Knowledge and Human Development},    url = {http://ijaseit.insightsociety.org/index.php?option=com_content&view=article&id=9&Itemid=1&article_id=936},    doi = {10.18517/ijaseit.7.4.936} }

EndNote

%A Siswoyo, Tri Agus
%A Matra, Nur Fauziah
%A Safiera, Anadini Aulia
%A Supriyadi, Anang
%D 2017
%T Synthesis of Antioxidant Peptides from Melinjo (Gnetum gnemon) Seed Protein Isolated Using Sol-Gel Immobilized Alcalase
%B 2017
%9 melinjo; immobilization; alcalase; antioxidant; peptide; Gnetum gnemon
%! Synthesis of Antioxidant Peptides from Melinjo (Gnetum gnemon) Seed Protein Isolated Using Sol-Gel Immobilized Alcalase
%K melinjo; immobilization; alcalase; antioxidant; peptide; Gnetum gnemon
%X 

The synthetic of antioxidant peptides from melinjo (Gnetum gnemon) seed protein isolated using sol-gel immobilized alcalase was studied, a commercial alcalase was physically entrapped in sol-gel matrices system. Characteristics of protein hydrolyzed were evaluated by the degree of hydrolysis obtained (DH) and the peptide profile by FPLC and SDS-PAGE. The antioxidant activity of protein hydrolyzed was determined using ABTS cation, DPPH, and superoxide radical scavenging assays. The DH values increased very quickly during the second hour followed by a slow increase up to 12 hours. Melinjo seed protein isolated was hydrolyzed about 23% of degree hydrolysis at 50°C after 2 hours of incubation. Similar results were also obtained by FPLC and SDS-PAGE, the peptide profile indicated the sol-gel immobilized alcalase was produced of peptides with low molecular weight from melinjo seed protein isolated. Immobilized alcalase preserved the initial activity over 4 cycles of repeated use in a routine reaction. The ABTS assay, the hydrolysate presented a more than  5 times greater activity than non-hydrolyzed melinjo seed protein isolated. It's confirming the potential use of sol-gel immobilized alcalase in synthetic antioxidant peptides from melinjo seed protein isolated. 

%U http://ijaseit.insightsociety.org/index.php?option=com_content&view=article&id=9&Itemid=1&article_id=936 %R doi:10.18517/ijaseit.7.4.936 %J International Journal on Advanced Science, Engineering and Information Technology %V 7 %N 4 %@ 2088-5334

IEEE

Tri Agus Siswoyo,Nur Fauziah Matra,Anadini Aulia Safiera and Anang Supriyadi,"Synthesis of Antioxidant Peptides from Melinjo (Gnetum gnemon) Seed Protein Isolated Using Sol-Gel Immobilized Alcalase," International Journal on Advanced Science, Engineering and Information Technology, vol. 7, no. 4, pp. 1315-1321, 2017. [Online]. Available: http://dx.doi.org/10.18517/ijaseit.7.4.936.

RefMan/ProCite (RIS)

TY  - JOUR
AU  - Siswoyo, Tri Agus
AU  - Matra, Nur Fauziah
AU  - Safiera, Anadini Aulia
AU  - Supriyadi, Anang
PY  - 2017
TI  - Synthesis of Antioxidant Peptides from Melinjo (Gnetum gnemon) Seed Protein Isolated Using Sol-Gel Immobilized Alcalase
JF  - International Journal on Advanced Science, Engineering and Information Technology; Vol. 7 (2017) No. 4
Y2  - 2017
SP  - 1315
EP  - 1321
SN  - 2088-5334
PB  - INSIGHT - Indonesian Society for Knowledge and Human Development
KW  - melinjo; immobilization; alcalase; antioxidant; peptide; Gnetum gnemon
N2  - 

The synthetic of antioxidant peptides from melinjo (Gnetum gnemon) seed protein isolated using sol-gel immobilized alcalase was studied, a commercial alcalase was physically entrapped in sol-gel matrices system. Characteristics of protein hydrolyzed were evaluated by the degree of hydrolysis obtained (DH) and the peptide profile by FPLC and SDS-PAGE. The antioxidant activity of protein hydrolyzed was determined using ABTS cation, DPPH, and superoxide radical scavenging assays. The DH values increased very quickly during the second hour followed by a slow increase up to 12 hours. Melinjo seed protein isolated was hydrolyzed about 23% of degree hydrolysis at 50°C after 2 hours of incubation. Similar results were also obtained by FPLC and SDS-PAGE, the peptide profile indicated the sol-gel immobilized alcalase was produced of peptides with low molecular weight from melinjo seed protein isolated. Immobilized alcalase preserved the initial activity over 4 cycles of repeated use in a routine reaction. The ABTS assay, the hydrolysate presented a more than  5 times greater activity than non-hydrolyzed melinjo seed protein isolated. It's confirming the potential use of sol-gel immobilized alcalase in synthetic antioxidant peptides from melinjo seed protein isolated. 

UR - http://ijaseit.insightsociety.org/index.php?option=com_content&view=article&id=9&Itemid=1&article_id=936 DO - 10.18517/ijaseit.7.4.936

RefWorks

RT Journal Article
ID 936
A1 Siswoyo, Tri Agus
A1 Matra, Nur Fauziah
A1 Safiera, Anadini Aulia
A1 Supriyadi, Anang
T1 Synthesis of Antioxidant Peptides from Melinjo (Gnetum gnemon) Seed Protein Isolated Using Sol-Gel Immobilized Alcalase
JF International Journal on Advanced Science, Engineering and Information Technology
VO 7
IS 4
YR 2017
SP 1315
OP 1321
SN 2088-5334
PB INSIGHT - Indonesian Society for Knowledge and Human Development
K1 melinjo; immobilization; alcalase; antioxidant; peptide; Gnetum gnemon
AB 

The synthetic of antioxidant peptides from melinjo (Gnetum gnemon) seed protein isolated using sol-gel immobilized alcalase was studied, a commercial alcalase was physically entrapped in sol-gel matrices system. Characteristics of protein hydrolyzed were evaluated by the degree of hydrolysis obtained (DH) and the peptide profile by FPLC and SDS-PAGE. The antioxidant activity of protein hydrolyzed was determined using ABTS cation, DPPH, and superoxide radical scavenging assays. The DH values increased very quickly during the second hour followed by a slow increase up to 12 hours. Melinjo seed protein isolated was hydrolyzed about 23% of degree hydrolysis at 50°C after 2 hours of incubation. Similar results were also obtained by FPLC and SDS-PAGE, the peptide profile indicated the sol-gel immobilized alcalase was produced of peptides with low molecular weight from melinjo seed protein isolated. Immobilized alcalase preserved the initial activity over 4 cycles of repeated use in a routine reaction. The ABTS assay, the hydrolysate presented a more than  5 times greater activity than non-hydrolyzed melinjo seed protein isolated. It's confirming the potential use of sol-gel immobilized alcalase in synthetic antioxidant peptides from melinjo seed protein isolated. 

LK http://ijaseit.insightsociety.org/index.php?option=com_content&view=article&id=9&Itemid=1&article_id=936 DO - 10.18517/ijaseit.7.4.936